| Microcystin-LR affects the hypothalamic-pituitary-inter-renal (HPI) axis in early life stages (embryos and larvae) of zebrafish | |
| Chen, Liang1,2; Wang, Yeke1,2; Giesy, John P.3,4; Chen, Feng1,2; Shi, Ting1,2; Chen, Jun1; Xie, Ping1,5 | |
| Corresponding Author | Xie, Ping(xieping@ihb.ac.cn) |
| 2018-10-01 | |
| Source Publication | ENVIRONMENTAL POLLUTION
 |
| ISSN | 0269-7491 |
| Volume | 241Pages:540-548 |
| Abstract | Frequencies and durations of blooms of cyanobacteria are increasing. Some cyanobacteria can produce cyanotoxins including microcystins (MCs). MCs are the most common toxic products of hazardous algal blooms (HABs), with the greatest potential for exposure and to cause toxicity. Recently, MCs have been shown to disrupt endocrine functions. In this study, for the first time, effects of MC-LR on the hypothalamic-pituitary-inter-renal (HPI) axis during early embryonic development (embryos/larvae) of zebrafish (Danio rerio), were investigated. Embryos/larvae of zebrafish were exposed to 1, 10, 100, or 30014 MC-LR/L during the period of 4-168 h post-fertilization (hpf). Exposure to 300 mu g MC-LR/L resulted in significantly greater concentrations of whole-body cortisol than those in controls. Expressions of genes along the HPI axis and mineralocorticoid receptor (MR-) and glucocorticoid receptor (GR-) centered gene networks were evaluated by use of quantitative real-time PCR Expression of mRNA for crh was significantly down-regulated by exposure to 300 mu g MC-LR/L, while expressions of crhbp, crhr1, and crhr2 were significantly up-regulated, relative to controls. MC-LR caused significantly lesser levels of mRNA for steroidogenic genes including hmgra, star, and cyp17, but expression of mRNA for hsd20b was significantly greater than that of controls. Treatment with MC-LR also altered profiles of transcription of MR- and GR-centered gene networks, which might result in multiple responses. Taken together, these results demonstrated that MC-LR affected the corticosteroid-endocrine system of larvae of zebrafish. This study provided valuable insights into molecular mechanisms behind potential toxicity and endocrine disruption of MCs. (C) 2018 Elsevier Ltd. All rights reserved. |
| Keyword | Hazardous algal blooms Cortisol Endocrine disruption Steroids Gene expression |
| DOI | 10.1016/j.envpol.2018.05.024 |
| Funding Organization | National Natural Science Foundations of China ; National Natural Science Foundations of China ; National Natural Science Foundations of China ; National Natural Science Foundations of China ; Qinghai Provincial Natural Science Foundation ; Qinghai Provincial Natural Science Foundation ; Qinghai Provincial Natural Science Foundation ; Qinghai Provincial Natural Science Foundation ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; High Level Foreign Experts program - State Administration of Foreign Experts Affairs, China ; High Level Foreign Experts program - State Administration of Foreign Experts Affairs, China ; High Level Foreign Experts program - State Administration of Foreign Experts Affairs, China ; High Level Foreign Experts program - State Administration of Foreign Experts Affairs, China ; Einstein Professor Program of the Chinese Academy of Sciences ; Einstein Professor Program of the Chinese Academy of Sciences ; Einstein Professor Program of the Chinese Academy of Sciences ; Einstein Professor Program of the Chinese Academy of Sciences ; Canada Research Chair program in Environmental Toxicology ; Canada Research Chair program in Environmental Toxicology ; Canada Research Chair program in Environmental Toxicology ; Canada Research Chair program in Environmental Toxicology ; Distinguished Visiting Professorship in the School of Biological Sciences of the University of Hong Kong ; Distinguished Visiting Professorship in the School of Biological Sciences of the University of Hong Kong ; Distinguished Visiting Professorship in the School of Biological Sciences of the University of Hong Kong ; Distinguished Visiting Professorship in the School of Biological Sciences of the University of Hong Kong ; National Natural Science Foundations of China ; National Natural Science Foundations of China ; National Natural Science Foundations of China ; National Natural Science Foundations of China ; Qinghai Provincial Natural Science Foundation ; Qinghai Provincial Natural Science Foundation ; Qinghai Provincial Natural Science Foundation ; Qinghai Provincial Natural Science Foundation ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; High Level Foreign Experts program - State Administration of Foreign Experts Affairs, China ; High Level Foreign Experts program - State Administration of Foreign Experts Affairs, China ; High Level Foreign Experts program - State Administration of Foreign Experts Affairs, China ; High Level Foreign Experts program - State Administration of Foreign Experts Affairs, China ; Einstein Professor Program of the Chinese Academy of Sciences ; Einstein Professor Program of the Chinese Academy of Sciences ; Einstein Professor Program of the Chinese Academy of Sciences ; Einstein Professor Program of the Chinese Academy of Sciences ; Canada Research Chair program in Environmental Toxicology ; Canada Research Chair program in Environmental Toxicology ; Canada Research Chair program in Environmental Toxicology ; Canada Research Chair program in Environmental Toxicology ; Distinguished Visiting Professorship in the School of Biological Sciences of the University of Hong Kong ; Distinguished Visiting Professorship in the School of Biological Sciences of the University of Hong Kong ; Distinguished Visiting Professorship in the School of Biological Sciences of the University of Hong Kong ; Distinguished Visiting Professorship in the School of Biological Sciences of the University of Hong Kong ; National Natural Science Foundations of China ; National Natural Science Foundations of China ; National Natural Science Foundations of China ; National Natural Science Foundations of China ; Qinghai Provincial Natural Science Foundation ; Qinghai Provincial Natural Science Foundation ; Qinghai Provincial Natural Science Foundation ; Qinghai Provincial Natural Science Foundation ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; High Level Foreign Experts program - State Administration of Foreign Experts Affairs, China ; High Level Foreign Experts program - State Administration of Foreign Experts Affairs, China ; High Level Foreign Experts program - State Administration of Foreign Experts Affairs, China ; High Level Foreign Experts program - State Administration of Foreign Experts Affairs, China ; Einstein Professor Program of the Chinese Academy of Sciences ; Einstein Professor Program of the Chinese Academy of Sciences ; Einstein Professor Program of the Chinese Academy of Sciences ; Einstein Professor Program of the Chinese Academy of Sciences ; Canada Research Chair program in Environmental Toxicology ; Canada Research Chair program in Environmental Toxicology ; Canada Research Chair program in Environmental Toxicology ; Canada Research Chair program in Environmental Toxicology ; Distinguished Visiting Professorship in the School of Biological Sciences of the University of Hong Kong ; Distinguished Visiting Professorship in the School of Biological Sciences of the University of Hong Kong ; Distinguished Visiting Professorship in the School of Biological Sciences of the University of Hong Kong ; Distinguished Visiting Professorship in the School of Biological Sciences of the University of Hong Kong ; National Natural Science Foundations of China ; National Natural Science Foundations of China ; National Natural Science Foundations of China ; National Natural Science Foundations of China ; Qinghai Provincial Natural Science Foundation ; Qinghai Provincial Natural Science Foundation ; Qinghai Provincial Natural Science Foundation ; Qinghai Provincial Natural Science Foundation ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; High Level Foreign Experts program - State Administration of Foreign Experts Affairs, China ; High Level Foreign Experts program - State Administration of Foreign Experts Affairs, China ; High Level Foreign Experts program - State Administration of Foreign Experts Affairs, China ; High Level Foreign Experts program - State Administration of Foreign Experts Affairs, China ; Einstein Professor Program of the Chinese Academy of Sciences ; Einstein Professor Program of the Chinese Academy of Sciences ; Einstein Professor Program of the Chinese Academy of Sciences ; Einstein Professor Program of the Chinese Academy of Sciences ; Canada Research Chair program in Environmental Toxicology ; Canada Research Chair program in Environmental Toxicology ; Canada Research Chair program in Environmental Toxicology ; Canada Research Chair program in Environmental Toxicology ; Distinguished Visiting Professorship in the School of Biological Sciences of the University of Hong Kong ; Distinguished Visiting Professorship in the School of Biological Sciences of the University of Hong Kong ; Distinguished Visiting Professorship in the School of Biological Sciences of the University of Hong Kong ; Distinguished Visiting Professorship in the School of Biological Sciences of the University of Hong Kong |
| Indexed By | SCI ; SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundations of China[31770555] ; Qinghai Provincial Natural Science Foundation[2018-ZJ-709] ; State Key Laboratory of Freshwater Ecology and Biotechnology[2016FBZ08] ; High Level Foreign Experts program - State Administration of Foreign Experts Affairs, China[GDT20143200016] ; Einstein Professor Program of the Chinese Academy of Sciences ; Canada Research Chair program in Environmental Toxicology ; Distinguished Visiting Professorship in the School of Biological Sciences of the University of Hong Kong |
| WOS Research Area | Environmental Sciences & Ecology |
| WOS Subject | Environmental Sciences |
| WOS ID | WOS:000440390800058 |
| WOS Keyword | ENVIRONMENTALLY RELEVANT CONCENTRATIONS ; CARP CTENOPHARYNGODON-IDELLA ; CORTICOSTEROID STRESS AXIS ; DANIO-RERIO ; THYROID-HORMONES ; FEMALE ZEBRAFISH ; REPRODUCTIVE TOXICITY ; ENDOCRINE DISRUPTION ; WATERBORNE EXPOSURE ; CARASSIUS-AURATUS |
| Publisher | ELSEVIER SCI LTD |
| Funding Organization | National Natural Science Foundations of China ; National Natural Science Foundations of China ; National Natural Science Foundations of China ; National Natural Science Foundations of China ; Qinghai Provincial Natural Science Foundation ; Qinghai Provincial Natural Science Foundation ; Qinghai Provincial Natural Science Foundation ; Qinghai Provincial Natural Science Foundation ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; High Level Foreign Experts program - State Administration of Foreign Experts Affairs, China ; High Level Foreign Experts program - State Administration of Foreign Experts Affairs, China ; High Level Foreign Experts program - State Administration of Foreign Experts Affairs, China ; High Level Foreign Experts program - State Administration of Foreign Experts Affairs, China ; Einstein Professor Program of the Chinese Academy of Sciences ; Einstein Professor Program of the Chinese Academy of Sciences ; Einstein Professor Program of the Chinese Academy of Sciences ; Einstein Professor Program of the Chinese Academy of Sciences ; Canada Research Chair program in Environmental Toxicology ; Canada Research Chair program in Environmental Toxicology ; Canada Research Chair program in Environmental Toxicology ; Canada Research Chair program in Environmental Toxicology ; Distinguished Visiting Professorship in the School of Biological Sciences of the University of Hong Kong ; Distinguished Visiting Professorship in the School of Biological Sciences of the University of Hong Kong ; Distinguished Visiting Professorship in the School of Biological Sciences of the University of Hong Kong ; Distinguished Visiting Professorship in the School of Biological Sciences of the University of Hong Kong ; National Natural Science Foundations of China ; National Natural Science Foundations of China ; National Natural Science Foundations of China ; National Natural Science Foundations of China ; Qinghai Provincial Natural Science Foundation ; Qinghai Provincial Natural Science Foundation ; Qinghai Provincial Natural Science Foundation ; Qinghai Provincial Natural Science Foundation ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; High Level Foreign Experts program - State Administration of Foreign Experts Affairs, China ; High Level Foreign Experts program - State Administration of Foreign Experts Affairs, China ; High Level Foreign Experts program - State Administration of Foreign Experts Affairs, China ; High Level Foreign Experts program - State Administration of Foreign Experts Affairs, China ; Einstein Professor Program of the Chinese Academy of Sciences ; Einstein Professor Program of the Chinese Academy of Sciences ; Einstein Professor Program of the Chinese Academy of Sciences ; Einstein Professor Program of the Chinese Academy of Sciences ; Canada Research Chair program in Environmental Toxicology ; Canada Research Chair program in Environmental Toxicology ; Canada Research Chair program in Environmental Toxicology ; Canada Research Chair program in Environmental Toxicology ; Distinguished Visiting Professorship in the School of Biological Sciences of the University of Hong Kong ; Distinguished Visiting Professorship in the School of Biological Sciences of the University of Hong Kong ; Distinguished Visiting Professorship in the School of Biological Sciences of the University of Hong Kong ; Distinguished Visiting Professorship in the School of Biological Sciences of the University of Hong Kong ; National Natural Science Foundations of China ; National Natural Science Foundations of China ; National Natural Science Foundations of China ; National Natural Science Foundations of China ; Qinghai Provincial Natural Science Foundation ; Qinghai Provincial Natural Science Foundation ; Qinghai Provincial Natural Science Foundation ; Qinghai Provincial Natural Science Foundation ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; High Level Foreign Experts program - State Administration of Foreign Experts Affairs, China ; High Level Foreign Experts program - State Administration of Foreign Experts Affairs, China ; High Level Foreign Experts program - State Administration of Foreign Experts Affairs, China ; High Level Foreign Experts program - State Administration of Foreign Experts Affairs, China ; Einstein Professor Program of the Chinese Academy of Sciences ; Einstein Professor Program of the Chinese Academy of Sciences ; Einstein Professor Program of the Chinese Academy of Sciences ; Einstein Professor Program of the Chinese Academy of Sciences ; Canada Research Chair program in Environmental Toxicology ; Canada Research Chair program in Environmental Toxicology ; Canada Research Chair program in Environmental Toxicology ; Canada Research Chair program in Environmental Toxicology ; Distinguished Visiting Professorship in the School of Biological Sciences of the University of Hong Kong ; Distinguished Visiting Professorship in the School of Biological Sciences of the University of Hong Kong ; Distinguished Visiting Professorship in the School of Biological Sciences of the University of Hong Kong ; Distinguished Visiting Professorship in the School of Biological Sciences of the University of Hong Kong ; National Natural Science Foundations of China ; National Natural Science Foundations of China ; National Natural Science Foundations of China ; National Natural Science Foundations of China ; Qinghai Provincial Natural Science Foundation ; Qinghai Provincial Natural Science Foundation ; Qinghai Provincial Natural Science Foundation ; Qinghai Provincial Natural Science Foundation ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; High Level Foreign Experts program - State Administration of Foreign Experts Affairs, China ; High Level Foreign Experts program - State Administration of Foreign Experts Affairs, China ; High Level Foreign Experts program - State Administration of Foreign Experts Affairs, China ; High Level Foreign Experts program - State Administration of Foreign Experts Affairs, China ; Einstein Professor Program of the Chinese Academy of Sciences ; Einstein Professor Program of the Chinese Academy of Sciences ; Einstein Professor Program of the Chinese Academy of Sciences ; Einstein Professor Program of the Chinese Academy of Sciences ; Canada Research Chair program in Environmental Toxicology ; Canada Research Chair program in Environmental Toxicology ; Canada Research Chair program in Environmental Toxicology ; Canada Research Chair program in Environmental Toxicology ; Distinguished Visiting Professorship in the School of Biological Sciences of the University of Hong Kong ; Distinguished Visiting Professorship in the School of Biological Sciences of the University of Hong Kong ; Distinguished Visiting Professorship in the School of Biological Sciences of the University of Hong Kong ; Distinguished Visiting Professorship in the School of Biological Sciences of the University of Hong Kong |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/30562 |
| Collection | 淡水生态学研究中心_期刊论文 |
| Corresponding Author | Xie, Ping |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Univ Saskatchewan, Dept Vet Biomed Sci, Saskatoon, SK S7N 5B3, Canada 4.Univ Saskatchewan, Toxicol Ctr, Saskatoon, SK S7N 5B3, Canada 5.Qinghai Univ, State Key Lab Plateau Ecol & Agr, Xining 810016, Qinghai, Peoples R China |
| Recommended Citation GB/T 7714 | Chen, Liang,Wang, Yeke,Giesy, John P.,et al. Microcystin-LR affects the hypothalamic-pituitary-inter-renal (HPI) axis in early life stages (embryos and larvae) of zebrafish[J]. ENVIRONMENTAL POLLUTION,2018,241:540-548. |
| APA | Chen, Liang.,Wang, Yeke.,Giesy, John P..,Chen, Feng.,Shi, Ting.,...&Xie, Ping.(2018).Microcystin-LR affects the hypothalamic-pituitary-inter-renal (HPI) axis in early life stages (embryos and larvae) of zebrafish.ENVIRONMENTAL POLLUTION,241,540-548. |
| MLA | Chen, Liang,et al."Microcystin-LR affects the hypothalamic-pituitary-inter-renal (HPI) axis in early life stages (embryos and larvae) of zebrafish".ENVIRONMENTAL POLLUTION 241(2018):540-548. |
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