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Geranylgeranylacetone induction of HSP90 alpha exerts cryoprotective effect on Acipenser sinensis sperm
Xi, Meng Dan1,2,3; Li, Ping3,4,5; Du, Hao3,4; Qiao, Xin Mei3,4; Liu, Zhi Gang3,4; Wei, Wei Qi1,3,4
Corresponding AuthorWei, Wei Qi(weiqw@yfi.ac.cn)
2018-06-01
Source PublicationANIMAL REPRODUCTION SCIENCE
ISSN0378-4320
Volume193Pages:19-25
AbstractHeat Shock Protein 90 (HSP90) is a fertility-associated protein, the expression of which positively correlates with sperm quality in many species. Geranylgeranylacetone (GGA) is reported to induce expression of HSP90. The present study aimed to investigate whether GGA induced expression of HSP90 in Acipenser sinensis sperm to exert a cryoprotective effect. Sperm from five male A. sinensis was combined with extender containing 20 mmol/L tris pH = 8.1, 10% v/v methanol, 2-5 mmol/L KC1, 15 mmol/L lactose, and 15 mmol/L trehalose, with GGA at 0, 14, 67, 135, 673, 1346, or 6731 mu mol/L. After cryopreservation and thawing, the percentage of motile spermatozoa, spermatozoon curvilinear velocity (VCL), straight-line velocity (VSL), average path velocity (VAP), acrosome integrity, and membrane integrity, as well as fertility were evaluated. Sperm quality increased with the increase of GGA to 673 mu mol/L, but decreased at higher concentrations. Expression levels of HSP90 alpha were detected by Western blot in sperm frozen with GGA at 673 mu mol/L (highest obtained sperm quality), 6731 mu mol/L (highest GGA concentration), and a control without GGA. The expression of HSP90 alpha increased with the increase in GGA, with lowest expression observed in the control. GGA was found to induce increase of HSP90 alpha, and this increase was associated with higher quality cryopreserved sperm at concentrations 5.673 mu mol/L. This research suggests a viable technique to increase the quality of cryopreserved A. sinensis sperm by adding GGA to induce expression of HSP90 alpha.
KeywordCryopreservation Geranylgeranylacetone HSP90 alpha Sperm quality Sturgeon
DOI10.1016/j.anireprosci.2018.03.027
Funding OrganizationSpecial Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; National Program on Key Basic Research Project (973 program) ; National Program on Key Basic Research Project (973 program) ; National Program on Key Basic Research Project (973 program) ; National Program on Key Basic Research Project (973 program) ; Ministry of Education, Youth and Sports of the Czech Republic ; Ministry of Education, Youth and Sports of the Czech Republic ; Ministry of Education, Youth and Sports of the Czech Republic ; Ministry of Education, Youth and Sports of the Czech Republic ; Czech Science Foundation ; Czech Science Foundation ; Czech Science Foundation ; Czech Science Foundation ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; National Program on Key Basic Research Project (973 program) ; National Program on Key Basic Research Project (973 program) ; National Program on Key Basic Research Project (973 program) ; National Program on Key Basic Research Project (973 program) ; Ministry of Education, Youth and Sports of the Czech Republic ; Ministry of Education, Youth and Sports of the Czech Republic ; Ministry of Education, Youth and Sports of the Czech Republic ; Ministry of Education, Youth and Sports of the Czech Republic ; Czech Science Foundation ; Czech Science Foundation ; Czech Science Foundation ; Czech Science Foundation ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; National Program on Key Basic Research Project (973 program) ; National Program on Key Basic Research Project (973 program) ; National Program on Key Basic Research Project (973 program) ; National Program on Key Basic Research Project (973 program) ; Ministry of Education, Youth and Sports of the Czech Republic ; Ministry of Education, Youth and Sports of the Czech Republic ; Ministry of Education, Youth and Sports of the Czech Republic ; Ministry of Education, Youth and Sports of the Czech Republic ; Czech Science Foundation ; Czech Science Foundation ; Czech Science Foundation ; Czech Science Foundation ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; National Program on Key Basic Research Project (973 program) ; National Program on Key Basic Research Project (973 program) ; National Program on Key Basic Research Project (973 program) ; National Program on Key Basic Research Project (973 program) ; Ministry of Education, Youth and Sports of the Czech Republic ; Ministry of Education, Youth and Sports of the Czech Republic ; Ministry of Education, Youth and Sports of the Czech Republic ; Ministry of Education, Youth and Sports of the Czech Republic ; Czech Science Foundation ; Czech Science Foundation ; Czech Science Foundation ; Czech Science Foundation
Indexed BySCI ; SCI
Language英语
Funding ProjectSpecial Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences[2015B02YQ01] ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences[2016GH04] ; National Natural Science Foundation of China[31402301] ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China[201203086] ; National Program on Key Basic Research Project (973 program)[2015CB150702] ; Ministry of Education, Youth and Sports of the Czech Republic[CZ.1.05/2.1.00/01.0024] ; Ministry of Education, Youth and Sports of the Czech Republic[L01205] ; Czech Science Foundation[16-02407Y]
WOS Research AreaAgriculture ; Reproductive Biology
WOS SubjectAgriculture, Dairy & Animal Science ; Reproductive Biology
WOS IDWOS:000433999000003
WOS KeywordHEAT-SHOCK PROTEINS ; ATP-BINDING-SITE ; HUMAN-SPERMATOZOA ; GENE-EXPRESSION ; BULL SPERM ; 90 HSP90 ; IN-VIVO ; MOTILITY ; CRYOPRESERVATION ; STURGEON
PublisherELSEVIER SCIENCE BV
Funding OrganizationSpecial Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; National Program on Key Basic Research Project (973 program) ; National Program on Key Basic Research Project (973 program) ; National Program on Key Basic Research Project (973 program) ; National Program on Key Basic Research Project (973 program) ; Ministry of Education, Youth and Sports of the Czech Republic ; Ministry of Education, Youth and Sports of the Czech Republic ; Ministry of Education, Youth and Sports of the Czech Republic ; Ministry of Education, Youth and Sports of the Czech Republic ; Czech Science Foundation ; Czech Science Foundation ; Czech Science Foundation ; Czech Science Foundation ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; National Program on Key Basic Research Project (973 program) ; National Program on Key Basic Research Project (973 program) ; National Program on Key Basic Research Project (973 program) ; National Program on Key Basic Research Project (973 program) ; Ministry of Education, Youth and Sports of the Czech Republic ; Ministry of Education, Youth and Sports of the Czech Republic ; Ministry of Education, Youth and Sports of the Czech Republic ; Ministry of Education, Youth and Sports of the Czech Republic ; Czech Science Foundation ; Czech Science Foundation ; Czech Science Foundation ; Czech Science Foundation ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; National Program on Key Basic Research Project (973 program) ; National Program on Key Basic Research Project (973 program) ; National Program on Key Basic Research Project (973 program) ; National Program on Key Basic Research Project (973 program) ; Ministry of Education, Youth and Sports of the Czech Republic ; Ministry of Education, Youth and Sports of the Czech Republic ; Ministry of Education, Youth and Sports of the Czech Republic ; Ministry of Education, Youth and Sports of the Czech Republic ; Czech Science Foundation ; Czech Science Foundation ; Czech Science Foundation ; Czech Science Foundation ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; National Program on Key Basic Research Project (973 program) ; National Program on Key Basic Research Project (973 program) ; National Program on Key Basic Research Project (973 program) ; National Program on Key Basic Research Project (973 program) ; Ministry of Education, Youth and Sports of the Czech Republic ; Ministry of Education, Youth and Sports of the Czech Republic ; Ministry of Education, Youth and Sports of the Czech Republic ; Ministry of Education, Youth and Sports of the Czech Republic ; Czech Science Foundation ; Czech Science Foundation ; Czech Science Foundation ; Czech Science Foundation
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/30481
Collection其他_期刊论文
Corresponding AuthorWei, Wei Qi
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Key Lab Freshwater Biodivers Conservat, Minist Agr China, Wuhan 430223, Hubei, Peoples R China
4.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Sinoczech Joint Lab Fish Conservat & Biotechnol, Wuhan 430223, Hubei, Peoples R China
5.Univ South Bohemia Ceske Budejovice, Fac Fisheries & Protect Waters, South Bohemian Res Ctr Aquaculture & Biodivers Hy, Res Inst Fish Culture & Hydrobiol, Zatisi 728-2, Vodnany 38925, Czech Republic
Recommended Citation
GB/T 7714
Xi, Meng Dan,Li, Ping,Du, Hao,et al. Geranylgeranylacetone induction of HSP90 alpha exerts cryoprotective effect on Acipenser sinensis sperm[J]. ANIMAL REPRODUCTION SCIENCE,2018,193:19-25.
APA Xi, Meng Dan,Li, Ping,Du, Hao,Qiao, Xin Mei,Liu, Zhi Gang,&Wei, Wei Qi.(2018).Geranylgeranylacetone induction of HSP90 alpha exerts cryoprotective effect on Acipenser sinensis sperm.ANIMAL REPRODUCTION SCIENCE,193,19-25.
MLA Xi, Meng Dan,et al."Geranylgeranylacetone induction of HSP90 alpha exerts cryoprotective effect on Acipenser sinensis sperm".ANIMAL REPRODUCTION SCIENCE 193(2018):19-25.
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