IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Distinct sperm nucleus behaviors between genotypic and temperature-dependent sex determination males are associated with replication and expression-related pathways in a gynogenetic fish
Zhu, Yao-Jun1; Li, Xi-Yin1; Zhang, Jun1; Li, Zhi1; Ding, Miao1; Zhang, Xiao-Juan1; Zhou, Li1; Gui, Jian-Fang1,2
Corresponding AuthorGui, Jian-Fang(jfgui@ihb.ac.cn)
2018-06-05
Source PublicationBMC GENOMICS
ISSN1471-2164
Volume19Pages:15
AbstractBackground: Coexistence and transition of diverse sex determination strategies have been revealed in some ectothermic species, but the variation between males caused by different sex determination strategies and the underlying mechanism remain unclear. Here, we used the gynogenetic gibel carp (Carassius gibelio) with both genotypic sex determination (GSD) and temperature-dependent sex determination (TSD) strategies to illustrate this issue. Results: We found out that males of GSD and TSD in gibel carp had similar morphology, testicular histology, sperm structure and sperm vitality. However, when maternal individuals were mated with males of GSD, sperm nucleus swelling and fusing with the female pronucleus were observed in the fertilized eggs. On the contrary, when maternal individuals were mated with males of TSD, sperm nucleus remained in the condensed status throughout the whole process. Subsequently, semen proteomics analysis unveiled that DNA replication and gene expression-related pathways were inhibited in the sperm from males of TSD compared to males of GSD, and most differentially expressed proteins associated with DNA replication, transcription and translation were down-regulated. Moreover, via BrdU incorporation and immunofluorescence detection, male nucleus replication was revealed to be present in the fertilized eggs by the sperm from males of GSD, but absent in the fertilized eggs by the sperm from males of TSD. Conclusions: These findings indicate that DNA replication and gene expression-related pathways are associated with the distinct sperm nucleus development behaviors in fertilized eggs in response to the sperm from males of GSD and TSD. And this study is the first attempt to screen the differences between males determined via GSD and TSD in gynogenetic species, which might give a hint for understanding evolutionary adaption of diverse sex determination mechanisms in unisexual vertebrates.
KeywordGenotypic sex determination Temperature-dependent sex determination Semen proteomics Gynogenesis
DOI10.1186/s12864-018-4823-6
Funding OrganizationKey Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science
Indexed BySCI ; SCI
Language英语
Funding ProjectKey Program of Frontier Sciences of the Chinese Academy of Sciences[QYZDY-SSW-SMC025] ; National Natural Science Foundation of China[31502148] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA08030201] ; Young Elite Scientist Sponsorship Program by CAST[YESS20150156] ; Earmarked Fund for Modern Agro-industry Technology Research System[NYCYTX-49] ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology[2016FBZ01] ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences[Y25A171] ; Knowledge Innovation Program of the Chinese Academy of Science
WOS Research AreaBiotechnology & Applied Microbiology ; Genetics & Heredity
WOS SubjectBiotechnology & Applied Microbiology ; Genetics & Heredity
WOS IDWOS:000434234700005
WOS KeywordCARASSIUS-AURATUS-GIBELIO ; PROTEOMIC ANALYSIS ; COMPLEX CYPRINIFORMES ; POLYPLOIDY ORIGINS ; REPRODUCTION MODES ; SPECIES COMPLEX ; GENETIC-BASIS ; CARP ; PROTEIN ; IDENTIFICATION
PublisherBIOMED CENTRAL LTD
Funding OrganizationKey Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science
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Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/30444
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorGui, Jian-Fang
Affiliation1.Univ Chinese Acad Sci, Chinese Acad Sci, Innovat Acad Seed Design, Inst Hydrobiol,State Key Lab Freshwater Ecol & Bi, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Zhu, Yao-Jun,Li, Xi-Yin,Zhang, Jun,et al. Distinct sperm nucleus behaviors between genotypic and temperature-dependent sex determination males are associated with replication and expression-related pathways in a gynogenetic fish[J]. BMC GENOMICS,2018,19:15.
APA Zhu, Yao-Jun.,Li, Xi-Yin.,Zhang, Jun.,Li, Zhi.,Ding, Miao.,...&Gui, Jian-Fang.(2018).Distinct sperm nucleus behaviors between genotypic and temperature-dependent sex determination males are associated with replication and expression-related pathways in a gynogenetic fish.BMC GENOMICS,19,15.
MLA Zhu, Yao-Jun,et al."Distinct sperm nucleus behaviors between genotypic and temperature-dependent sex determination males are associated with replication and expression-related pathways in a gynogenetic fish".BMC GENOMICS 19(2018):15.
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