| Distinct sperm nucleus behaviors between genotypic and temperature-dependent sex determination males are associated with replication and expression-related pathways in a gynogenetic fish | |
| Zhu, Yao-Jun1; Li, Xi-Yin1; Zhang, Jun1; Li, Zhi1; Ding, Miao1; Zhang, Xiao-Juan1; Zhou, Li1; Gui, Jian-Fang1,2 | |
| Corresponding Author | Gui, Jian-Fang(jfgui@ihb.ac.cn) |
| 2018-06-05 | |
| Source Publication | BMC GENOMICS
 |
| ISSN | 1471-2164 |
| Volume | 19Pages:15 |
| Abstract | Background: Coexistence and transition of diverse sex determination strategies have been revealed in some ectothermic species, but the variation between males caused by different sex determination strategies and the underlying mechanism remain unclear. Here, we used the gynogenetic gibel carp (Carassius gibelio) with both genotypic sex determination (GSD) and temperature-dependent sex determination (TSD) strategies to illustrate this issue. Results: We found out that males of GSD and TSD in gibel carp had similar morphology, testicular histology, sperm structure and sperm vitality. However, when maternal individuals were mated with males of GSD, sperm nucleus swelling and fusing with the female pronucleus were observed in the fertilized eggs. On the contrary, when maternal individuals were mated with males of TSD, sperm nucleus remained in the condensed status throughout the whole process. Subsequently, semen proteomics analysis unveiled that DNA replication and gene expression-related pathways were inhibited in the sperm from males of TSD compared to males of GSD, and most differentially expressed proteins associated with DNA replication, transcription and translation were down-regulated. Moreover, via BrdU incorporation and immunofluorescence detection, male nucleus replication was revealed to be present in the fertilized eggs by the sperm from males of GSD, but absent in the fertilized eggs by the sperm from males of TSD. Conclusions: These findings indicate that DNA replication and gene expression-related pathways are associated with the distinct sperm nucleus development behaviors in fertilized eggs in response to the sperm from males of GSD and TSD. And this study is the first attempt to screen the differences between males determined via GSD and TSD in gynogenetic species, which might give a hint for understanding evolutionary adaption of diverse sex determination mechanisms in unisexual vertebrates. |
| Keyword | Genotypic sex determination Temperature-dependent sex determination Semen proteomics Gynogenesis |
| DOI | 10.1186/s12864-018-4823-6 |
| Funding Organization | Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science |
| Indexed By | SCI ; SCI |
| Language | 英语 |
| Funding Project | Key Program of Frontier Sciences of the Chinese Academy of Sciences[QYZDY-SSW-SMC025] ; National Natural Science Foundation of China[31502148] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA08030201] ; Young Elite Scientist Sponsorship Program by CAST[YESS20150156] ; Earmarked Fund for Modern Agro-industry Technology Research System[NYCYTX-49] ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology[2016FBZ01] ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences[Y25A171] ; Knowledge Innovation Program of the Chinese Academy of Science |
| WOS Research Area | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| WOS Subject | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| WOS ID | WOS:000434234700005 |
| WOS Keyword | CARASSIUS-AURATUS-GIBELIO ; PROTEOMIC ANALYSIS ; COMPLEX CYPRINIFORMES ; POLYPLOIDY ORIGINS ; REPRODUCTION MODES ; SPECIES COMPLEX ; GENETIC-BASIS ; CARP ; PROTEIN ; IDENTIFICATION |
| Publisher | BIOMED CENTRAL LTD |
| Funding Organization | Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Young Elite Scientist Sponsorship Program by CAST ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Project of the Institute of Hydrobiology, Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science ; Knowledge Innovation Program of the Chinese Academy of Science |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/30444 |
| Collection | 鱼类生物学及渔业生物技术研究中心_期刊论文 |
| Corresponding Author | Gui, Jian-Fang |
| Affiliation | 1.Univ Chinese Acad Sci, Chinese Acad Sci, Innovat Acad Seed Design, Inst Hydrobiol,State Key Lab Freshwater Ecol & Bi, Wuhan 430072, Hubei, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China |
| Recommended Citation GB/T 7714 | Zhu, Yao-Jun,Li, Xi-Yin,Zhang, Jun,et al. Distinct sperm nucleus behaviors between genotypic and temperature-dependent sex determination males are associated with replication and expression-related pathways in a gynogenetic fish[J]. BMC GENOMICS,2018,19:15. |
| APA | Zhu, Yao-Jun.,Li, Xi-Yin.,Zhang, Jun.,Li, Zhi.,Ding, Miao.,...&Gui, Jian-Fang.(2018).Distinct sperm nucleus behaviors between genotypic and temperature-dependent sex determination males are associated with replication and expression-related pathways in a gynogenetic fish.BMC GENOMICS,19,15. |
| MLA | Zhu, Yao-Jun,et al."Distinct sperm nucleus behaviors between genotypic and temperature-dependent sex determination males are associated with replication and expression-related pathways in a gynogenetic fish".BMC GENOMICS 19(2018):15. |
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