IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Expression analysis of immune genes in goldfish (Carassius auratus) infected with the monogenean parasite Gyrodactylus kobayashii
Zhou, Shun1,2; Li, Wen X.1; Zou, Hong1; Zhang, Jing1; Wu, Shan G.1; Li, Ming1; Wang, Gui T.1,2
Corresponding AuthorLi, Wen X.(liwx@ihb.ac.cn)
2018-06-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume77Pages:40-45
AbstractHeavy infection with the ectoparasite Gyrodactylus kobayashii commonly leads to high mortality of ornamental goldfish Carassius auratus. To understand the underlying mechanisms of goldfish against infection of gyrodactylids, transcription responses of immune-related genes including IL-1 beta 2, TNF alpha 1, TNF alpha 2, IFN-gamma, TGF beta, MHC II beta, TCR beta 1 and complement factor C3 were studied by real-time quantitative PCR analysis. Significant increases of expression of inflammatory genes such as IL-1 beta 2, TNF alpha 1, TNF alpha 2 and TGF beta were detected at days 7 and 14 post-infection (pi). No significant differences of G. kobayashii load were observed in response to primary infection and re-infection. In addition, the transcript levels of genes involved in adaptive immunity such as MHC II beta and TCR beta 1 remained unchanged. Curiously, fish moderately infected with gyrodactylid showed elevated expression IL-1 beta 2, TNFa alpha 1 and TNF alpha 2. In all the gyrodactylids-infected fish, expression of complement factor C3 was consistently inhibited. The results extend current knowledge to the understanding of gyrodactylid infection in fish and support the previous findings that innate immunity is indispensable for controlling parasite infection.
KeywordGyrodactylus kobayashii Goldfish Infection Immune gene transcription
DOI10.1016/j.fsi.2018.03.033
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; major scientific and technological innovation project of Hubei Province ; major scientific and technological innovation project of Hubei Province ; major scientific and technological innovation project of Hubei Province ; major scientific and technological innovation project of Hubei Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; major scientific and technological innovation project of Hubei Province ; major scientific and technological innovation project of Hubei Province ; major scientific and technological innovation project of Hubei Province ; major scientific and technological innovation project of Hubei Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; major scientific and technological innovation project of Hubei Province ; major scientific and technological innovation project of Hubei Province ; major scientific and technological innovation project of Hubei Province ; major scientific and technological innovation project of Hubei Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; major scientific and technological innovation project of Hubei Province ; major scientific and technological innovation project of Hubei Province ; major scientific and technological innovation project of Hubei Province ; major scientific and technological innovation project of Hubei Province
Indexed BySCI ; SCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31572658] ; Earmarked Fund for China Agriculture Research System[CARS-45-15] ; major scientific and technological innovation project of Hubei Province[2015ABA045]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000432644300006
WOS KeywordTROUT ONCORHYNCHUS-MYKISS ; POECILIA-RETICULATA PETERS ; SALMO-SALAR L. ; CHALLENGE INFECTIONS ; ATLANTIC SALMON ; RESPONSE GENES ; SKIN ; BULLATARUDIS ; GUPPIES ; PLATYHELMINTHES
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; major scientific and technological innovation project of Hubei Province ; major scientific and technological innovation project of Hubei Province ; major scientific and technological innovation project of Hubei Province ; major scientific and technological innovation project of Hubei Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; major scientific and technological innovation project of Hubei Province ; major scientific and technological innovation project of Hubei Province ; major scientific and technological innovation project of Hubei Province ; major scientific and technological innovation project of Hubei Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; major scientific and technological innovation project of Hubei Province ; major scientific and technological innovation project of Hubei Province ; major scientific and technological innovation project of Hubei Province ; major scientific and technological innovation project of Hubei Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; major scientific and technological innovation project of Hubei Province ; major scientific and technological innovation project of Hubei Province ; major scientific and technological innovation project of Hubei Province ; major scientific and technological innovation project of Hubei Province
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/30441
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorLi, Wen X.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Key Lab Aquaculture Dis Control,Minist Agr, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Zhou, Shun,Li, Wen X.,Zou, Hong,et al. Expression analysis of immune genes in goldfish (Carassius auratus) infected with the monogenean parasite Gyrodactylus kobayashii[J]. FISH & SHELLFISH IMMUNOLOGY,2018,77:40-45.
APA Zhou, Shun.,Li, Wen X..,Zou, Hong.,Zhang, Jing.,Wu, Shan G..,...&Wang, Gui T..(2018).Expression analysis of immune genes in goldfish (Carassius auratus) infected with the monogenean parasite Gyrodactylus kobayashii.FISH & SHELLFISH IMMUNOLOGY,77,40-45.
MLA Zhou, Shun,et al."Expression analysis of immune genes in goldfish (Carassius auratus) infected with the monogenean parasite Gyrodactylus kobayashii".FISH & SHELLFISH IMMUNOLOGY 77(2018):40-45.
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