| Transcriptomic Profiles of Brain Provide Insights into Molecular Mechanism of Feed Conversion Efficiency in Crucian Carp (Carassius auratus) | |
Pang, Meixia1,2; Luo, Weiwei1; Fu, Beide1 ; Yu, Xiaomu1; Zhou, Ying1,2; Tong, Jingou1
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| Corresponding Author | Tong, Jingou(jgtong@ihb.ac.cn) |
| 2018-03-01 | |
| Source Publication | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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| ISSN | 1422-0067 |
| Volume | 19Issue:3Pages:14 |
| Abstract | Feed efficiency is an economically crucial trait for cultured animals, however, progress has been scarcely made in the genetic analyses of feed conversion efficiency (FCE) in fish because of the difficulties in measurement of trait phenotypes. In the present investigation, we present the first application of RNA sequencing (RNA-Seq) combined with differentially expressed genes (DEGs) analysis for identification of functional determinants related to FCE at the gene level in an aquaculture fish, crucian carp (Carassius auratus). Brain tissues of six crucian carp with extreme FCE performances were subjected to transcriptome analysis. A total of 544,612 unigenes with a mean size of 644.38 bp were obtained from Low- and High-FCE groups, and 246 DEGs that may be involved in FCE traits were identified in these two groups. qPCR confirmed that genes previously identified as up- or down-regulated by RNA-Seq were effectively up- or down-regulated under the studied conditions. Thirteen key genes, whose functions are associated with metabolism (Dgkk, Mgst3 and Guk1b), signal transduction (Vdnccsalb, Tgf alpha, Nr4a1 and Tacr2) and growth (Endog, Crebrtc2, Myh7, Myh1, Myh14 and Igfbp7) were identified according to GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) annotations. Our novel findings provide useful pathway information and candidate genes for future studies of genetic mechanisms underlying FCE in crucian carp. |
| Keyword | crucian carp (Carassius auratus) feed conversion efficiency brain differentially expressed genes RNA-Seq |
| DOI | 10.3390/ijms19030858 |
| Funding Organization | Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology |
| Indexed By | SCI ; SCI |
| Language | 英语 |
| Funding Project | Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences[XDA08010405] ; State Key Laboratory of Freshwater Ecology and Biotechnology[2016FBZ05] |
| WOS Research Area | Biochemistry & Molecular Biology ; Chemistry |
| WOS Subject | Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary |
| WOS ID | WOS:000428309800215 |
| WOS Keyword | QUANTITATIVE TRAIT LOCI ; CANDIDATE GENES ; CLIMATE-CHANGE ; BEEF-CATTLE ; GENOME ; IDENTIFICATION ; GROWTH ; FOOD ; PERSPECTIVES ; ASSOCIATION |
| Publisher | MDPI |
| Funding Organization | Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/30435 |
| Collection | 鱼类生物学及渔业生物技术研究中心_期刊论文 |
| Corresponding Author | Tong, Jingou |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| Recommended Citation GB/T 7714 | Pang, Meixia,Luo, Weiwei,Fu, Beide,et al. Transcriptomic Profiles of Brain Provide Insights into Molecular Mechanism of Feed Conversion Efficiency in Crucian Carp (Carassius auratus)[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2018,19(3):14. |
| APA | Pang, Meixia,Luo, Weiwei,Fu, Beide,Yu, Xiaomu,Zhou, Ying,&Tong, Jingou.(2018).Transcriptomic Profiles of Brain Provide Insights into Molecular Mechanism of Feed Conversion Efficiency in Crucian Carp (Carassius auratus).INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,19(3),14. |
| MLA | Pang, Meixia,et al."Transcriptomic Profiles of Brain Provide Insights into Molecular Mechanism of Feed Conversion Efficiency in Crucian Carp (Carassius auratus)".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 19.3(2018):14. |
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