IHB OpenIR  > 藻类生物技术和生物能源研发中心  > 期刊论文
An orthogonal system for heterologous expression of actinobacterial lasso peptides in Streptomyces hosts
Mevaere, Jimmy1; Goulard, Christophe1; Schneider, Olha2; Sekurova, Olga N.3; Ma, Haiyan1,5; Zirah, Severine1; Afonso, Carlos4; Rebuffat, Sylvie1; Zotchev, Sergey B.3; Li, Yanyan1
2018-05-29
Source PublicationSCIENTIFIC REPORTS
ISSN2045-2322
Volume8Issue:1Pages:11
Abstract

Lasso peptides are ribosomally synthesized and post-translationally modified peptides produced by bacteria. They are characterized by an unusual lariat-knot structure. Targeted genome scanning revealed a wide diversity of lasso peptides encoded in actinobacterial genomes, but cloning and heterologous expression of these clusters turned out to be problematic. To circumvent this, we developed an orthogonal expression system for heterologous production of actinobacterial lasso peptides in Streptomyces hosts based on a newly-identified regulatory circuit from Actinoalloteichus fjordicus. Six lasso peptide gene clusters, mainly originating from marine Actinobacteria, were chosen for proof-of-concept studies. By varying the Streptomyces expression hosts and a small set of culture conditions, three new lasso peptides were successfully produced and characterized by tandem MS. The newly developed expression system thus sets the stage to uncover and bioengineer the chemodiversity of actinobacterial lasso peptides. Moreover, our data provide some considerations for future bioprospecting efforts for such peptides.

DOI10.1038/s41598-018-26620-0
Funding OrganizationEuropean Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris
Indexed BySCI
Language英语
Funding ProjectEuropean Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH[613877] ; University of Vienna ; NTNU ; City of Paris
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000433290000011
WOS KeywordBIOSYNTHETIC GENE CLUSTERS ; BACTERIAL RNA-POLYMERASE ; GENOME-MINING APPROACH ; MASS-SPECTROMETRY ; NATURAL-PRODUCTS ; MICROCIN J25 ; BIOLOGICAL-ACTIVITY ; SIAMYCIN I ; NOMENCLATURE ; IONS
PublisherNATURE PUBLISHING GROUP
Funding OrganizationEuropean Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/30429
Collection藻类生物技术和生物能源研发中心_期刊论文
Corresponding AuthorZotchev, Sergey B.; Li, Yanyan
Affiliation1.Sorbonne Univ, CNRS, Lab Mol Commun & Adaptat Microorganisms, MCAM,UMR 7245,MNHN, CP 54,57 Rue Cuvier, F-75005 Paris, France
2.Norwegian Univ Sci & Technol NTNU, Dept Biotechnol, N-7491 Trondheim, Norway
3.Univ Vienna, Dept Pharmacognosy, Althanstr 14, A-1090 Vienna, Austria
4.Normandie Univ, CNRS, UNIROUEN, INSA Rouen,COBRA, Rouen, France
5.Chinese Acad Sci, Inst Hydrobiol, Ctr Microalgal Biotechnol & Biofuels, Wuhan, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Mevaere, Jimmy,Goulard, Christophe,Schneider, Olha,et al. An orthogonal system for heterologous expression of actinobacterial lasso peptides in Streptomyces hosts[J]. SCIENTIFIC REPORTS,2018,8(1):11.
APA Mevaere, Jimmy.,Goulard, Christophe.,Schneider, Olha.,Sekurova, Olga N..,Ma, Haiyan.,...&Li, Yanyan.(2018).An orthogonal system for heterologous expression of actinobacterial lasso peptides in Streptomyces hosts.SCIENTIFIC REPORTS,8(1),11.
MLA Mevaere, Jimmy,et al."An orthogonal system for heterologous expression of actinobacterial lasso peptides in Streptomyces hosts".SCIENTIFIC REPORTS 8.1(2018):11.
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