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Characterization of Microcystis (Cyanobacteria) Genotypes Based on the Internal Transcribed Spacer Region of rRNA by Next-Generation Sequencing
Huo, Da1,2; Chen, Youxin2; Zheng, Tao1; Liu, Xiang2; Zhang, Xinyue1; Yu, Gongliang2; Qiao, Zhiyi1; Li, Renhui2
Corresponding AuthorQiao, Zhiyi(qiaozhiyi7070@163.com) ; Li, Renhui(reli@ihb.ac.cn)
2018-05-15
Source PublicationFRONTIERS IN MICROBIOLOGY
ISSN1664-302X
Volume9Pages:12
AbstractMicrocystis is one of the most common and dominant bloom-forming cyanobacteria in freshwater worldwide. The method for genotype detection based on traditional molecular cloning is expensive and time consuming and generates a limited number of sequences. In this study, a high-throughput sequencing (HTS) method was developed to detect the internal transcribed spacer (ITS) regions between 16S and 23S rRNA region of Microcystis populations along a typical water system in Yuqiao Reservoir-Haihe River in Tianjin, northern China. A total of 629,341 reads were obtained and clustered into 2005 operational taxonomic units (OTUs). Analysis of alpha diversity indices showed that the Haihe River is more diverse than Yuqiao Reservoir. In general, the two water areas exhibit a clear differentiation pattern in OTU abundance, sharing genotypes from a small part of Yuqiao Reservoir with those in the Haihe River. Phylogenetic analysis further indicated the possible flexible evolution of Microcystis genotypes occurring in the research areas. This study provides the first exhaustive description of HTS method for detection of ITS region to evaluate Microcystis intra-species diversity and relationship.
Keywordcyanobacteria Yuqiao reservoir Haihe River high throughput sequencing ITS region
DOI10.3389/fmicb.2018.00971
Funding OrganizationNational Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; International Science and Technology Cooperation Program of China ; International Science and Technology Cooperation Program of China ; International Science and Technology Cooperation Program of China ; International Science and Technology Cooperation Program of China ; Tianjin Agricultural University Key Laboratory of Aqua-ecology platform project ; Tianjin Agricultural University Key Laboratory of Aqua-ecology platform project ; Tianjin Agricultural University Key Laboratory of Aqua-ecology platform project ; Tianjin Agricultural University Key Laboratory of Aqua-ecology platform project ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; International Science and Technology Cooperation Program of China ; International Science and Technology Cooperation Program of China ; International Science and Technology Cooperation Program of China ; International Science and Technology Cooperation Program of China ; Tianjin Agricultural University Key Laboratory of Aqua-ecology platform project ; Tianjin Agricultural University Key Laboratory of Aqua-ecology platform project ; Tianjin Agricultural University Key Laboratory of Aqua-ecology platform project ; Tianjin Agricultural University Key Laboratory of Aqua-ecology platform project ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; International Science and Technology Cooperation Program of China ; International Science and Technology Cooperation Program of China ; International Science and Technology Cooperation Program of China ; International Science and Technology Cooperation Program of China ; Tianjin Agricultural University Key Laboratory of Aqua-ecology platform project ; Tianjin Agricultural University Key Laboratory of Aqua-ecology platform project ; Tianjin Agricultural University Key Laboratory of Aqua-ecology platform project ; Tianjin Agricultural University Key Laboratory of Aqua-ecology platform project ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; International Science and Technology Cooperation Program of China ; International Science and Technology Cooperation Program of China ; International Science and Technology Cooperation Program of China ; International Science and Technology Cooperation Program of China ; Tianjin Agricultural University Key Laboratory of Aqua-ecology platform project ; Tianjin Agricultural University Key Laboratory of Aqua-ecology platform project ; Tianjin Agricultural University Key Laboratory of Aqua-ecology platform project ; Tianjin Agricultural University Key Laboratory of Aqua-ecology platform project
Indexed BySCI ; SCI
Language英语
Funding ProjectNational Science Foundation of China[NSFC51779247] ; International Science and Technology Cooperation Program of China[2013DFA71340] ; Tianjin Agricultural University Key Laboratory of Aqua-ecology platform project
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000432290000001
WOS KeywordREAL-TIME PCR ; GENETIC DIVERSITY ; LAKE TAIHU ; TOXIC CYANOBACTERIUM ; SPATIOTEMPORAL CHANGES ; ENVIRONMENTAL-FACTORS ; BLOOM ; POPULATIONS ; CHINA ; AERUGINOSA
PublisherFRONTIERS MEDIA SA
Funding OrganizationNational Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; International Science and Technology Cooperation Program of China ; International Science and Technology Cooperation Program of China ; International Science and Technology Cooperation Program of China ; International Science and Technology Cooperation Program of China ; Tianjin Agricultural University Key Laboratory of Aqua-ecology platform project ; Tianjin Agricultural University Key Laboratory of Aqua-ecology platform project ; Tianjin Agricultural University Key Laboratory of Aqua-ecology platform project ; Tianjin Agricultural University Key Laboratory of Aqua-ecology platform project ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; International Science and Technology Cooperation Program of China ; International Science and Technology Cooperation Program of China ; International Science and Technology Cooperation Program of China ; International Science and Technology Cooperation Program of China ; Tianjin Agricultural University Key Laboratory of Aqua-ecology platform project ; Tianjin Agricultural University Key Laboratory of Aqua-ecology platform project ; Tianjin Agricultural University Key Laboratory of Aqua-ecology platform project ; Tianjin Agricultural University Key Laboratory of Aqua-ecology platform project ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; International Science and Technology Cooperation Program of China ; International Science and Technology Cooperation Program of China ; International Science and Technology Cooperation Program of China ; International Science and Technology Cooperation Program of China ; Tianjin Agricultural University Key Laboratory of Aqua-ecology platform project ; Tianjin Agricultural University Key Laboratory of Aqua-ecology platform project ; Tianjin Agricultural University Key Laboratory of Aqua-ecology platform project ; Tianjin Agricultural University Key Laboratory of Aqua-ecology platform project ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; National Science Foundation of China ; International Science and Technology Cooperation Program of China ; International Science and Technology Cooperation Program of China ; International Science and Technology Cooperation Program of China ; International Science and Technology Cooperation Program of China ; Tianjin Agricultural University Key Laboratory of Aqua-ecology platform project ; Tianjin Agricultural University Key Laboratory of Aqua-ecology platform project ; Tianjin Agricultural University Key Laboratory of Aqua-ecology platform project ; Tianjin Agricultural University Key Laboratory of Aqua-ecology platform project
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/30401
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorQiao, Zhiyi; Li, Renhui
Affiliation1.Tianjin Agr Univ, Dept Fisheries Sci, Natl Demonstrat Ctr Expt Aquaecol & Aquaculture E, Tianjin, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Huo, Da,Chen, Youxin,Zheng, Tao,et al. Characterization of Microcystis (Cyanobacteria) Genotypes Based on the Internal Transcribed Spacer Region of rRNA by Next-Generation Sequencing[J]. FRONTIERS IN MICROBIOLOGY,2018,9:12.
APA Huo, Da.,Chen, Youxin.,Zheng, Tao.,Liu, Xiang.,Zhang, Xinyue.,...&Li, Renhui.(2018).Characterization of Microcystis (Cyanobacteria) Genotypes Based on the Internal Transcribed Spacer Region of rRNA by Next-Generation Sequencing.FRONTIERS IN MICROBIOLOGY,9,12.
MLA Huo, Da,et al."Characterization of Microcystis (Cyanobacteria) Genotypes Based on the Internal Transcribed Spacer Region of rRNA by Next-Generation Sequencing".FRONTIERS IN MICROBIOLOGY 9(2018):12.
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