IHB OpenIR  > 水环境工程研究中心  > 期刊论文
An enzymatic mechanism for balancing the stoichiometry of nitrogen and phosphorus in a shallow Chinese eutrophic lake
Xiao, Jian1,2; Wang, Si-Yang1,2; Zhou, Zi-Jun1,2; Zhang, Yao1,2; Song, Chun-Lei1; Zhou, Yi-Yong1; Cao, Xiu-Yun1
Corresponding AuthorCao, Xiu-Yun(caoxy@ihb.ac.cn)
2018-07-15
Source PublicationSCIENCE OF THE TOTAL ENVIRONMENT
ISSN0048-9697
Volume630Pages:1071-1077
AbstractThe over-enrichment of lake waters with nitrogen (N) and phosphorus (P) has become a serious environmental problem, but modes of change in stoichiometry and enzymatic regeneration along trophic gradients are largely unknown. Seasonal variations in the kinetics of extracellular aminopeptidase (LAP) and alkaline phosphatase (AP), together with the composition of phytoplankton and concentrations of N and P, were examined from Jun 2013 to September 2014 in a Chinese shallow lake in which two basins had contrasting trophic states. The turbid basin had a significantly higher concentration of chlorophyll a and lower ratios of N to P. In parallel, the turnover time of organic N mediated by LAP (T-LAP) was significantly shorter, and its maximum velocity (V-max) was significantly higher compared to those in the clear basin. Considering data from both basins, there were linear decreases in N/P and the ratios between dissolved inorganic N and total N with an increasing trophic state index, coupled with a significantly positive relationship between N/P and LAPT. Additionally, with decreasing TN/TP, the Michaelis constant (K-m) of the AP increased linearly, reducing the efficiency of P regeneration. In contrast, the Km value of LAP decreased, and V-max increased, which enhanced Nmineralization by simultaneously increasing the reaction velocity and improving the affinity for substrate. Additionally, the K-m value of LAP was significantly related to that of AP and the ammonium concentration. Thus, substrate affinity acted as a key factor modifying the pathways of enzymatic degradation of organic N and P according to their stoichiometry in the water column. Phytoplankton composition was directly linked to LAPT. Overall, this study seemed to be the first to connect a stoichiometric shift of N and P with kinetics of extracellular enzymes responsible for their regeneration along trophic gradients, presenting an additional pathway to overcome nitrogen deficiency in eutrophic lakes. (C) 2018 Elsevier B.V. All rights reserved.
KeywordStoichiometry of nitrogen and phosphorus Nitrogen deficiency Alkaline phosphatase Leucine aminopeptidase Michaelis-Menten kinetics Eutrophication
DOI10.1016/j.scitotenv.2018.02.297
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology
Indexed BySCI ; SCI
Language英语
Funding ProjectNational Natural Science Foundation of China[41573110] ; National Natural Science Foundation of China[41230748] ; National Natural Science Foundation of China[41173081] ; Major Science and Technology Program for Water Pollution Control and Treatment[2017ZX07603] ; State Key Laboratory of Freshwater Ecology and Biotechnology[2016FBZ07]
WOS Research AreaEnvironmental Sciences & Ecology
WOS SubjectEnvironmental Sciences
WOS IDWOS:000432467700105
WOS KeywordAMINOPEPTIDASE ACTIVITY ; ALKALINE-PHOSPHATASE ; NUTRIENT LIMITATION ; BACTERIAL-ACTIVITY ; TROPHIC GRADIENT ; ORGANIC-MATTER ; SEDIMENT ; KINETICS ; PHYTOPLANKTON ; RATIOS
PublisherELSEVIER SCIENCE BV
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; Major Science and Technology Program for Water Pollution Control and Treatment ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/30398
Collection水环境工程研究中心_期刊论文
Corresponding AuthorCao, Xiu-Yun
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Key Lab Algal Biol, 7 Donghu South Rd, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Xiao, Jian,Wang, Si-Yang,Zhou, Zi-Jun,et al. An enzymatic mechanism for balancing the stoichiometry of nitrogen and phosphorus in a shallow Chinese eutrophic lake[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2018,630:1071-1077.
APA Xiao, Jian.,Wang, Si-Yang.,Zhou, Zi-Jun.,Zhang, Yao.,Song, Chun-Lei.,...&Cao, Xiu-Yun.(2018).An enzymatic mechanism for balancing the stoichiometry of nitrogen and phosphorus in a shallow Chinese eutrophic lake.SCIENCE OF THE TOTAL ENVIRONMENT,630,1071-1077.
MLA Xiao, Jian,et al."An enzymatic mechanism for balancing the stoichiometry of nitrogen and phosphorus in a shallow Chinese eutrophic lake".SCIENCE OF THE TOTAL ENVIRONMENT 630(2018):1071-1077.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Xiao, Jian]'s Articles
[Wang, Si-Yang]'s Articles
[Zhou, Zi-Jun]'s Articles
Baidu academic
Similar articles in Baidu academic
[Xiao, Jian]'s Articles
[Wang, Si-Yang]'s Articles
[Zhou, Zi-Jun]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Xiao, Jian]'s Articles
[Wang, Si-Yang]'s Articles
[Zhou, Zi-Jun]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.