| Fish-mediated changes in bacterioplankton community composition: an in situ mesocosm experiment | |
Luo Congqiang1,2; Yi Chunlong1; Ni Leyi1; Guo Longgen1
| |
| Corresponding Author | Guo Longgen(longgen@ihb.ac.cn) |
| 2018-03-01 | |
| Source Publication | JOURNAL OF OCEANOLOGY AND LIMNOLOGY
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| ISSN | 2096-5508 |
| Volume | 36Issue:2Pages:341-350 |
| Abstract | We characterized variations in bacterioplankton community composition (BCC) in mesocosms subject to three different treatments. Two groups contained fish (group one: Cyprinus carpio; group two: Hypophthalmichthys molitrix); and group three, the untreated mesocosm, was the control. Samples were taken seven times over a 49-d period, and BCC was analyzed by PCR-denaturing gradient gel electrophoresis (DGGE) and real-time quantitative PCR (qPCR). Results revealed that introduction of C. carpio and H. molitrix had a remarkable impact on the composition of bacterioplankton communities, and the BCC was significantly different between each treatment. Sequencing of DGGE bands revealed that the bacterioplankton community in the different treatment groups was consistent at a taxonomic level, but differed in its abundance. H. molitrix promoted the richness of Alphaproteobacteria and Actinobacteria, while more bands affiliated to Cyanobacteria were detected in C. carpio mesocosms. The redundancy analysis (RDA) result demonstrated that the BCC was closely related to the bottom-up (total phosphorus, chlorophyll a, phytoplankton biomass) and top-down forces (biomass of copepods and cladocera) in C. carpio and control mesocosms, respectively. We found no evidence for top-down regulation of BCC by zooplankton in H. molitrix mesocosms, while grazing by protozoa (heterotrophic nanoflagellates, ciliates) became the major way to regulate BCC. Total bacterioplankton abundances were significantly higher in C. carpio mesocosms because of high nutrient concentration and suspended solids. Our study provided insights into the relationship between fish and bacterioplankton at species level, leading to a deep understanding of the function of the microbial loop and the aquatic ecosystem. |
| Keyword | bacterioplankton community composition (BCC) PCR-DGGE qPCR analysis of similarities (ANOSIM) redundancy analysis (RDA) |
| DOI | 10.1007/s00343-018-6281-z |
| Funding Organization | National Key Technology R&D Program of China ; National Key Technology R&D Program of China ; National Key Technology R&D Program of China ; National Key Technology R&D Program of China ; National Water Pollution Control and Management Technology Major Projects ; National Water Pollution Control and Management Technology Major Projects ; National Water Pollution Control and Management Technology Major Projects ; National Water Pollution Control and Management Technology Major Projects ; High-Level Scientific Research Foundation for the Introduction of Talent ; High-Level Scientific Research Foundation for the Introduction of Talent ; High-Level Scientific Research Foundation for the Introduction of Talent ; High-Level Scientific Research Foundation for the Introduction of Talent ; National Key Technology R&D Program of China ; National Key Technology R&D Program of China ; National Key Technology R&D Program of China ; National Key Technology R&D Program of China ; National Water Pollution Control and Management Technology Major Projects ; National Water Pollution Control and Management Technology Major Projects ; National Water Pollution Control and Management Technology Major Projects ; National Water Pollution Control and Management Technology Major Projects ; High-Level Scientific Research Foundation for the Introduction of Talent ; High-Level Scientific Research Foundation for the Introduction of Talent ; High-Level Scientific Research Foundation for the Introduction of Talent ; High-Level Scientific Research Foundation for the Introduction of Talent ; National Key Technology R&D Program of China ; National Key Technology R&D Program of China ; National Key Technology R&D Program of China ; National Key Technology R&D Program of China ; National Water Pollution Control and Management Technology Major Projects ; National Water Pollution Control and Management Technology Major Projects ; National Water Pollution Control and Management Technology Major Projects ; National Water Pollution Control and Management Technology Major Projects ; High-Level Scientific Research Foundation for the Introduction of Talent ; High-Level Scientific Research Foundation for the Introduction of Talent ; High-Level Scientific Research Foundation for the Introduction of Talent ; High-Level Scientific Research Foundation for the Introduction of Talent ; National Key Technology R&D Program of China ; National Key Technology R&D Program of China ; National Key Technology R&D Program of China ; National Key Technology R&D Program of China ; National Water Pollution Control and Management Technology Major Projects ; National Water Pollution Control and Management Technology Major Projects ; National Water Pollution Control and Management Technology Major Projects ; National Water Pollution Control and Management Technology Major Projects ; High-Level Scientific Research Foundation for the Introduction of Talent ; High-Level Scientific Research Foundation for the Introduction of Talent ; High-Level Scientific Research Foundation for the Introduction of Talent ; High-Level Scientific Research Foundation for the Introduction of Talent |
| Indexed By | SCI ; SCI |
| Language | 英语 |
| Funding Project | National Key Technology R&D Program of China[2014BAC09B02] ; National Water Pollution Control and Management Technology Major Projects[2012ZX07101-002] ; High-Level Scientific Research Foundation for the Introduction of Talent[E07016043] |
| WOS Research Area | Marine & Freshwater Biology ; Oceanography |
| WOS Subject | Limnology ; Oceanography |
| WOS ID | WOS:000431662200014 |
| WOS Keyword | MICROBIAL FOOD-WEB ; BACTERIAL COMMUNITY ; LAKE ; SHALLOW ; PHYTOPLANKTON ; CHINA ; PREDATION ; PATTERNS ; BIOMASS ; MARINE |
| Publisher | SCIENCE PRESS |
| Funding Organization | National Key Technology R&D Program of China ; National Key Technology R&D Program of China ; National Key Technology R&D Program of China ; National Key Technology R&D Program of China ; National Water Pollution Control and Management Technology Major Projects ; National Water Pollution Control and Management Technology Major Projects ; National Water Pollution Control and Management Technology Major Projects ; National Water Pollution Control and Management Technology Major Projects ; High-Level Scientific Research Foundation for the Introduction of Talent ; High-Level Scientific Research Foundation for the Introduction of Talent ; High-Level Scientific Research Foundation for the Introduction of Talent ; High-Level Scientific Research Foundation for the Introduction of Talent ; National Key Technology R&D Program of China ; National Key Technology R&D Program of China ; National Key Technology R&D Program of China ; National Key Technology R&D Program of China ; National Water Pollution Control and Management Technology Major Projects ; National Water Pollution Control and Management Technology Major Projects ; National Water Pollution Control and Management Technology Major Projects ; National Water Pollution Control and Management Technology Major Projects ; High-Level Scientific Research Foundation for the Introduction of Talent ; High-Level Scientific Research Foundation for the Introduction of Talent ; High-Level Scientific Research Foundation for the Introduction of Talent ; High-Level Scientific Research Foundation for the Introduction of Talent ; National Key Technology R&D Program of China ; National Key Technology R&D Program of China ; National Key Technology R&D Program of China ; National Key Technology R&D Program of China ; National Water Pollution Control and Management Technology Major Projects ; National Water Pollution Control and Management Technology Major Projects ; National Water Pollution Control and Management Technology Major Projects ; National Water Pollution Control and Management Technology Major Projects ; High-Level Scientific Research Foundation for the Introduction of Talent ; High-Level Scientific Research Foundation for the Introduction of Talent ; High-Level Scientific Research Foundation for the Introduction of Talent ; High-Level Scientific Research Foundation for the Introduction of Talent ; National Key Technology R&D Program of China ; National Key Technology R&D Program of China ; National Key Technology R&D Program of China ; National Key Technology R&D Program of China ; National Water Pollution Control and Management Technology Major Projects ; National Water Pollution Control and Management Technology Major Projects ; National Water Pollution Control and Management Technology Major Projects ; National Water Pollution Control and Management Technology Major Projects ; High-Level Scientific Research Foundation for the Introduction of Talent ; High-Level Scientific Research Foundation for the Introduction of Talent ; High-Level Scientific Research Foundation for the Introduction of Talent ; High-Level Scientific Research Foundation for the Introduction of Talent |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/30310 |
| Collection | 淡水生态学研究中心_期刊论文 |
| Corresponding Author | Guo Longgen |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Hubei, Peoples R China 2.Hunan Univ Arts & Sci, Collaborat Innovat Ctr Efficient & Hlth Prod Fi, Key Lab Hlth Aquaculture & Prod Proc Dongtin Lake, Changde 415000, Peoples R China |
| Recommended Citation GB/T 7714 | Luo Congqiang,Yi Chunlong,Ni Leyi,et al. Fish-mediated changes in bacterioplankton community composition: an in situ mesocosm experiment[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2018,36(2):341-350. |
| APA | Luo Congqiang,Yi Chunlong,Ni Leyi,&Guo Longgen.(2018).Fish-mediated changes in bacterioplankton community composition: an in situ mesocosm experiment.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,36(2),341-350. |
| MLA | Luo Congqiang,et al."Fish-mediated changes in bacterioplankton community composition: an in situ mesocosm experiment".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 36.2(2018):341-350. |
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