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Fish-mediated changes in bacterioplankton community composition: an in situ mesocosm experiment
Luo Congqiang1,2; Yi Chunlong1; Ni Leyi1; Guo Longgen1
2018-03-01
Source PublicationJOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN2096-5508
Volume36Issue:2Pages:341-350
Abstract

We characterized variations in bacterioplankton community composition (BCC) in mesocosms subject to three different treatments. Two groups contained fish (group one: Cyprinus carpio; group two: Hypophthalmichthys molitrix); and group three, the untreated mesocosm, was the control. Samples were taken seven times over a 49-d period, and BCC was analyzed by PCR-denaturing gradient gel electrophoresis (DGGE) and real-time quantitative PCR (qPCR). Results revealed that introduction of C. carpio and H. molitrix had a remarkable impact on the composition of bacterioplankton communities, and the BCC was significantly different between each treatment. Sequencing of DGGE bands revealed that the bacterioplankton community in the different treatment groups was consistent at a taxonomic level, but differed in its abundance. H. molitrix promoted the richness of Alphaproteobacteria and Actinobacteria, while more bands affiliated to Cyanobacteria were detected in C. carpio mesocosms. The redundancy analysis (RDA) result demonstrated that the BCC was closely related to the bottom-up (total phosphorus, chlorophyll a, phytoplankton biomass) and top-down forces (biomass of copepods and cladocera) in C. carpio and control mesocosms, respectively. We found no evidence for top-down regulation of BCC by zooplankton in H. molitrix mesocosms, while grazing by protozoa (heterotrophic nanoflagellates, ciliates) became the major way to regulate BCC. Total bacterioplankton abundances were significantly higher in C. carpio mesocosms because of high nutrient concentration and suspended solids. Our study provided insights into the relationship between fish and bacterioplankton at species level, leading to a deep understanding of the function of the microbial loop and the aquatic ecosystem.

Keywordbacterioplankton community composition (BCC) PCR-DGGE qPCR analysis of similarities (ANOSIM) redundancy analysis (RDA)
DOI10.1007/s00343-018-6281-z
Funding OrganizationNational Key Technology R&D Program of China ; National Key Technology R&D Program of China ; National Key Technology R&D Program of China ; National Key Technology R&D Program of China ; National Key Technology R&D Program of China ; National Key Technology R&D Program of China ; National Key Technology R&D Program of China ; National Key Technology R&D Program of China ; National Water Pollution Control and Management Technology Major Projects ; National Water Pollution Control and Management Technology Major Projects ; National Water Pollution Control and Management Technology Major Projects ; National Water Pollution Control and Management Technology Major Projects ; National Water Pollution Control and Management Technology Major Projects ; National Water Pollution Control and Management Technology Major Projects ; National Water Pollution Control and Management Technology Major Projects ; National Water Pollution Control and Management Technology Major Projects ; 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Indexed BySCI
Language英语
Funding ProjectNational Key Technology R&D Program of China[2014BAC09B02] ; National Water Pollution Control and Management Technology Major Projects[2012ZX07101-002] ; High-Level Scientific Research Foundation for the Introduction of Talent[E07016043]
WOS Research AreaMarine & Freshwater Biology ; Oceanography
WOS SubjectLimnology ; Oceanography
WOS IDWOS:000431662200014
WOS KeywordMICROBIAL FOOD-WEB ; BACTERIAL COMMUNITY ; LAKE ; SHALLOW ; PHYTOPLANKTON ; CHINA ; PREDATION ; PATTERNS ; BIOMASS ; MARINE
PublisherSCIENCE PRESS
Funding OrganizationNational Key Technology R&D Program of China ; National Key Technology R&D Program of China ; National Key Technology R&D Program of China ; National Key Technology R&D Program of China ; National Key Technology R&D Program of China ; National Key Technology R&D Program of China ; National Key Technology R&D Program of China ; National Key Technology R&D Program of China ; National Water Pollution Control and Management Technology Major Projects ; National Water Pollution Control and Management Technology Major Projects ; National Water Pollution Control and Management Technology Major Projects ; National Water Pollution Control and Management Technology Major Projects ; National Water Pollution Control and Management Technology Major Projects ; National Water Pollution Control and Management Technology Major Projects ; National Water Pollution Control and Management Technology Major Projects ; National Water Pollution Control and Management Technology Major Projects ; 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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/30310
Collection淡水生态学研究中心_期刊论文
Corresponding AuthorGuo Longgen
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Hubei, Peoples R China
2.Hunan Univ Arts & Sci, Collaborat Innovat Ctr Efficient & Hlth Prod Fi, Key Lab Hlth Aquaculture & Prod Proc Dongtin Lake, Changde 415000, Peoples R China
Recommended Citation
GB/T 7714
Luo Congqiang,Yi Chunlong,Ni Leyi,et al. Fish-mediated changes in bacterioplankton community composition: an in situ mesocosm experiment[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2018,36(2):341-350.
APA Luo Congqiang,Yi Chunlong,Ni Leyi,&Guo Longgen.(2018).Fish-mediated changes in bacterioplankton community composition: an in situ mesocosm experiment.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,36(2),341-350.
MLA Luo Congqiang,et al."Fish-mediated changes in bacterioplankton community composition: an in situ mesocosm experiment".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 36.2(2018):341-350.
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