IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Transcriptome analysis of Rana chensinensis liver under trichlorfon stress
Ma, Yu1,2; Li, Bo1,2; Ke, Yang2; Zhang, Yongan3; Zhang, Yuhui1
2018
Source PublicationECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
ISSN0147-6513
Volume147Issue:1Pages:487-493
Abstract

Trichlorfon is a selective organophosphate insecticide that is widely applied in aquaculture and agriculture for control of various parasites. However, repeated and excess applications of trichlorfon often lead to water pollution and threaten non-targeted species. Our previous studies showed that trichlorfon could cause oxidative stress, lipid peroxidation and hepatic lesions in the liver of Rana chensinensis, but the related molecular mechanisms remain unclear. To explore the interference of trichlorfon in gene transcription, the differentially expressed genes in the liver of R. chensinensis exposed to trichlorfon were characterized using the RNA-seq platform. A search of all unigenes against non-redundant protein sequence (Nr), non-redundant nucleotide (Nt), Swiss-Prot, Kyoto Encyclopaedia of Genes and Genomes (KEGG), Clusters of Orthologous Groups (COG) and Gene Ontology (GO) databases resulted in 22,888, 21,719, 20,934, 16,923, 7375 and 15,631 annotations, respectively, and provided a total of 27,781 annotated unigenes. Among the annotated unigenes, 16,923 were mapped to 257 signalling pathways. A set of 3329 differentially expressed unigenes was identified by comparison of the two groups in liver. Notably, relative expression of metabolism-related genes, including both up and down-regulated genes, were also validated by qPCR. The present study depicts the high degree of transcriptional complexity in R. chensinensis under trichlorfon stress and provides new insights into the molecular mechanisms of organophosphate insecticide toxicology. Some of these metabolism-responsive genes could be useful for understanding the toxicological mechanism of trichlorfon on non-target aquatic organisms and will contribute to the conservation of aquatic life.

KeywordOrganophosphate insecticides Trichlorfon Differentially expressed genes Rana chensinensis RNA-seq
DOI10.1016/j.ecoenv.2017.09.016
Funding OrganizationCAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science
Indexed BySCI
Language英语
Funding ProjectCAS Light of West China visiting scholar training project ; Foundation of the Shaanxi Academy of Science[2017k-05] ; Foundation of the Shaanxi Academy of Science[2017k-27]
WOS Research AreaEnvironmental Sciences & Ecology ; Toxicology
WOS SubjectEnvironmental Sciences ; Toxicology
WOS IDWOS:000416199700060
WOS KeywordPACU PIARACTUS-MESOPOTAMICUS ; GENE-EXPRESSION ; RNA-SEQ ; OXIDATIVE STRESS ; CYTOCHROME-P450 GENES ; INSECTICIDE ; RESISTANCE ; FROG ; ISCHEMIA/REPERFUSION ; OVEREXPRESSION
PublisherACADEMIC PRESS INC ELSEVIER SCIENCE
Funding OrganizationCAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; CAS Light of West China visiting scholar training project ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science ; Foundation of the Shaanxi Academy of Science
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Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/30221
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorZhang, Yuhui
Affiliation1.Shaanxi Normal Univ, Coll Life Sci, Xian 710062, Shaanxi, Peoples R China
2.Shaanxi Microbiol Inst, Xian 710043, Shaanxi, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Ma, Yu,Li, Bo,Ke, Yang,et al. Transcriptome analysis of Rana chensinensis liver under trichlorfon stress[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2018,147(1):487-493.
APA Ma, Yu,Li, Bo,Ke, Yang,Zhang, Yongan,&Zhang, Yuhui.(2018).Transcriptome analysis of Rana chensinensis liver under trichlorfon stress.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,147(1),487-493.
MLA Ma, Yu,et al."Transcriptome analysis of Rana chensinensis liver under trichlorfon stress".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 147.1(2018):487-493.
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