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Chlamydomonas IFT25 is dispensable for flagellar assembly but required to export the BBSome from flagella
Dong, Bin1; Wu, Song1; Wang, Jing1; Liu, Yan-Xia1; Peng, Zhao3; Meng, De-Mei1; Huang, Kaiyao3; Wu, Mingfu2; Fan, Zhen-Chuan1,4
2017-11-01
Source PublicationBIOLOGY OPEN
ISSN2046-6390
Volume6Issue:11Pages:1680-1691
Abstract

Intraflagellar transport (IFT) particles are composed of polyprotein complexes IFT-A and IFT-B as well as cargo adaptors such as the BBSome. Two IFT-B subunits, IFT25 and IFT27 were found to form a heterodimer, which is essential in exporting the BBSome out of the cilium but not involved in flagellar assembly and cytokinesis in vertebrates. Controversial results were, however, recorded to show that defects in IFT, flagellar assembly and even cytokinesis were caused by IFT27 knockdown in Chlamydomonas reinhardtii. Using C. reinhardtii as a model organism, we report that depletion of IFT25 has no effect on flagellar assembly and does not affect the entry of the BBSome into the flagellum, but IFT25 depletion did impair BBSome movement out of the flagellum, clarifying the evolutionally conserved role of IFT25 in regulating the exit of the BBSome from the flagellum cross species. Interestingly, depletion of IFT25 causes dramatic reduction of IFT27 as expected, which does not cause defects in flagellar assembly and cytokinesis in C. reinhardtii. Our data thus support that Chlamydomonas IFT27, like its vertebrate homologues, is not involved in flagellar assembly and cytokinesis.

KeywordChlamydomonas reinhardtii Intraflagellar transport IFT protein Flagellar assembly BBSome
DOI10.1242/bio.026278
Funding OrganizationInternational Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB)
Indexed BySCI
Language英语
Funding ProjectInternational Center for Genetic Engineering and Biotechnology (ICGEB)[CRP/CHN15-01]
WOS Research AreaLife Sciences & Biomedicine - Other Topics
WOS SubjectBiology
WOS IDWOS:000417557800010
WOS KeywordRETROGRADE INTRAFLAGELLAR TRANSPORT ; BARDET-BIEDL-SYNDROME ; CAENORHABDITIS-ELEGANS ; COMPLEX-B ; CYTOPLASMIC DYNEIN ; SYNDROME PROTEINS ; BASAL BODY ; CILIA ; REINHARDTII ; PARTICLES
PublisherCOMPANY OF BIOLOGISTS LTD
Funding OrganizationInternational Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB)
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/30164
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorFan, Zhen-Chuan
Affiliation1.Tianjin Univ Sci & Technol, Int Collaborat Res Ctr Hlth Biotechnol, Coll Food Engn & Biotechnol, Inst Hlth Biotechnol,Minist Educ China,Key Lab Foo, Tianjin 300457, Peoples R China
2.Albany Med Coll, Cardiovasc Sci Ctr, Albany, NY 12208 USA
3.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China
4.Obesita & Algaegen LLC, College Stn, TX 77845 USA
Recommended Citation
GB/T 7714
Dong, Bin,Wu, Song,Wang, Jing,et al. Chlamydomonas IFT25 is dispensable for flagellar assembly but required to export the BBSome from flagella[J]. BIOLOGY OPEN,2017,6(11):1680-1691.
APA Dong, Bin.,Wu, Song.,Wang, Jing.,Liu, Yan-Xia.,Peng, Zhao.,...&Fan, Zhen-Chuan.(2017).Chlamydomonas IFT25 is dispensable for flagellar assembly but required to export the BBSome from flagella.BIOLOGY OPEN,6(11),1680-1691.
MLA Dong, Bin,et al."Chlamydomonas IFT25 is dispensable for flagellar assembly but required to export the BBSome from flagella".BIOLOGY OPEN 6.11(2017):1680-1691.
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