Chlamydomonas IFT25 is dispensable for flagellar assembly but required to export the BBSome from flagella | |
Dong, Bin1; Wu, Song1; Wang, Jing1; Liu, Yan-Xia1; Peng, Zhao3; Meng, De-Mei1; Huang, Kaiyao3; Wu, Mingfu2; Fan, Zhen-Chuan1,4 | |
2017-11-01 | |
Source Publication | BIOLOGY OPEN
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ISSN | 2046-6390 |
Volume | 6Issue:11Pages:1680-1691 |
Abstract | Intraflagellar transport (IFT) particles are composed of polyprotein complexes IFT-A and IFT-B as well as cargo adaptors such as the BBSome. Two IFT-B subunits, IFT25 and IFT27 were found to form a heterodimer, which is essential in exporting the BBSome out of the cilium but not involved in flagellar assembly and cytokinesis in vertebrates. Controversial results were, however, recorded to show that defects in IFT, flagellar assembly and even cytokinesis were caused by IFT27 knockdown in Chlamydomonas reinhardtii. Using C. reinhardtii as a model organism, we report that depletion of IFT25 has no effect on flagellar assembly and does not affect the entry of the BBSome into the flagellum, but IFT25 depletion did impair BBSome movement out of the flagellum, clarifying the evolutionally conserved role of IFT25 in regulating the exit of the BBSome from the flagellum cross species. Interestingly, depletion of IFT25 causes dramatic reduction of IFT27 as expected, which does not cause defects in flagellar assembly and cytokinesis in C. reinhardtii. Our data thus support that Chlamydomonas IFT27, like its vertebrate homologues, is not involved in flagellar assembly and cytokinesis. |
Keyword | Chlamydomonas reinhardtii Intraflagellar transport IFT protein Flagellar assembly BBSome |
DOI | 10.1242/bio.026278 |
Funding Organization | International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) |
Indexed By | SCI |
Language | 英语 |
Funding Project | International Center for Genetic Engineering and Biotechnology (ICGEB)[CRP/CHN15-01] |
WOS Research Area | Life Sciences & Biomedicine - Other Topics |
WOS Subject | Biology |
WOS ID | WOS:000417557800010 |
WOS Keyword | RETROGRADE INTRAFLAGELLAR TRANSPORT ; BARDET-BIEDL-SYNDROME ; CAENORHABDITIS-ELEGANS ; COMPLEX-B ; CYTOPLASMIC DYNEIN ; SYNDROME PROTEINS ; BASAL BODY ; CILIA ; REINHARDTII ; PARTICLES |
Publisher | COMPANY OF BIOLOGISTS LTD |
Funding Organization | International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) ; International Center for Genetic Engineering and Biotechnology (ICGEB) |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.ihb.ac.cn/handle/342005/30164 |
Collection | 藻类生物学及应用研究中心_期刊论文 |
Corresponding Author | Fan, Zhen-Chuan |
Affiliation | 1.Tianjin Univ Sci & Technol, Int Collaborat Res Ctr Hlth Biotechnol, Coll Food Engn & Biotechnol, Inst Hlth Biotechnol,Minist Educ China,Key Lab Foo, Tianjin 300457, Peoples R China 2.Albany Med Coll, Cardiovasc Sci Ctr, Albany, NY 12208 USA 3.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China 4.Obesita & Algaegen LLC, College Stn, TX 77845 USA |
Recommended Citation GB/T 7714 | Dong, Bin,Wu, Song,Wang, Jing,et al. Chlamydomonas IFT25 is dispensable for flagellar assembly but required to export the BBSome from flagella[J]. BIOLOGY OPEN,2017,6(11):1680-1691. |
APA | Dong, Bin.,Wu, Song.,Wang, Jing.,Liu, Yan-Xia.,Peng, Zhao.,...&Fan, Zhen-Chuan.(2017).Chlamydomonas IFT25 is dispensable for flagellar assembly but required to export the BBSome from flagella.BIOLOGY OPEN,6(11),1680-1691. |
MLA | Dong, Bin,et al."Chlamydomonas IFT25 is dispensable for flagellar assembly but required to export the BBSome from flagella".BIOLOGY OPEN 6.11(2017):1680-1691. |
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Dong-2017-Chlamydomo(2607KB) | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text |
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