IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Essential roles of &ITstat5.1&IT/&ITstat5b&IT in controlling fish somatic growth
Xiong, Shuting1; Mei, Jie1; Huang, Peipei2; Jing, Jing1,3; Li, Zhi2; Kang, Jingliang2; Gui, Jian-Fang1,2
Corresponding AuthorMei, Jie(jmei@mail.hzau.edu.cn) ; Gui, Jian-Fang(jfgui@ihb.ac.cn)
2017-12-20
Source PublicationJOURNAL OF GENETICS AND GENOMICS
ISSN1673-8527
Volume44Issue:12Pages:577-585
AbstractSignal transducer and activator of transcription 5b (STAT5b) has been identified as a key downstream mediator of growth hormone (GH) signaling in somatic growth of mammalian. However, the corresponding homologue gene of Stat5b is unknown in fish species. In this study, we generated loss-of-function mutants in stat5.1 and stat5.2, two stat5 homologues existing in zebrafish. In stat5.1-deficient zebrafish, a significant reduction of body length and body weight was detected in the embryos/larvae and adults compared with the wild-type control fish, and sexual size dimorphism in adult zebrafish was also eliminated. However, the stat5.2-deficient zebrafish displayed a normal developmental phenotype during all lifespan. Chromatin immunoprecipitation combined with deep sequencing (ChIP-seq) method was adopted to further investigate the potential transcriptional targets of Stat5 protein and cast much light upon the biological function of Stat5. We identified more than 800 genes as transcriptional targets of Stat5 during zebrafish embryogenesis. KEGG analysis indicated that the Stat5 target gene network is predominantly linked to the metabolic pathways, neuroactive ligand-receptor interaction and JAK-STAT signaling pathways. Further validation studies suggested that Stat5.1 protein could directly regulate the expression of gilt and stat5.1-mutated zebrafish showed a reduction of gh1 mRNA level. In the present study, stat5.1 was revealed as the corresponding homologue gene of Stat5b in fish species. Additionally, we found a novel molecular interaction between Stat5.1/Stat5b and GH, and unraveled a positive feedback loop Stat5.1-GH-Stat5.1 which is necessary for somatic growth and body development in zebrafish. Copyright (C) 2017, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Limited and Science Press. All rights reserved.
KeywordZebrafish Stat5 Body growth Sexual size dimorphism Growth hormone
DOI10.1016/j.jgg.2017.07.006
Funding OrganizationAutonomous Projects of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Projects of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Projects of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Projects of the Institute of Hydrobiology, Chinese Academy of Sciences ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Projects of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Projects of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Projects of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Projects of the Institute of Hydrobiology, Chinese Academy of Sciences ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Projects of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Projects of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Projects of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Projects of the Institute of Hydrobiology, Chinese Academy of Sciences ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Projects of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Projects of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Projects of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Projects of the Institute of Hydrobiology, Chinese Academy of Sciences ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology
Indexed BySCI ; SCI
Language英语
Funding ProjectAutonomous Projects of the Institute of Hydrobiology, Chinese Academy of Sciences[Y25A17] ; Autonomous Projects of the Institute of Hydrobiology, Chinese Academy of Sciences[Y45A171301] ; Fundamental Research Funds for the Central Universities[2662017PY013] ; Fundamental Research Funds for the Central Universities[2662015PY101] ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology[2011FBZ22]
WOS Research AreaBiochemistry & Molecular Biology ; Genetics & Heredity
WOS SubjectBiochemistry & Molecular Biology ; Genetics & Heredity
WOS IDWOS:000418884000003
WOS KeywordSEXUAL SIZE DIMORPHISM ; GH/IGF AXIS GENES ; YELLOW CATFISH ; FACTOR-I ; BONE-DEVELOPMENT ; HORMONE ; ZEBRAFISH ; EXPRESSION ; LIVER ; CARP
PublisherSCIENCE PRESS
Funding OrganizationAutonomous Projects of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Projects of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Projects of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Projects of the Institute of Hydrobiology, Chinese Academy of Sciences ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Projects of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Projects of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Projects of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Projects of the Institute of Hydrobiology, Chinese Academy of Sciences ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Projects of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Projects of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Projects of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Projects of the Institute of Hydrobiology, Chinese Academy of Sciences ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Projects of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Projects of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Projects of the Institute of Hydrobiology, Chinese Academy of Sciences ; Autonomous Projects of the Institute of Hydrobiology, Chinese Academy of Sciences ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of State Key Laboratory of Freshwater Ecology and Biotechnology
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Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/30112
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorMei, Jie; Gui, Jian-Fang
Affiliation1.Huazhong Agr Univ, Minist Agr, Key Lab Freshwater Anim Breeding, Coll Fisheries, Wuhan 430070, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
3.Wuhan Univ, Med Res Inst, Wuhan 430071, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Xiong, Shuting,Mei, Jie,Huang, Peipei,et al. Essential roles of &ITstat5.1&IT/&ITstat5b&IT in controlling fish somatic growth[J]. JOURNAL OF GENETICS AND GENOMICS,2017,44(12):577-585.
APA Xiong, Shuting.,Mei, Jie.,Huang, Peipei.,Jing, Jing.,Li, Zhi.,...&Gui, Jian-Fang.(2017).Essential roles of &ITstat5.1&IT/&ITstat5b&IT in controlling fish somatic growth.JOURNAL OF GENETICS AND GENOMICS,44(12),577-585.
MLA Xiong, Shuting,et al."Essential roles of &ITstat5.1&IT/&ITstat5b&IT in controlling fish somatic growth".JOURNAL OF GENETICS AND GENOMICS 44.12(2017):577-585.
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