IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Systematic Identification of Mycobacterium tuberculosis Effectors Reveals that BfrB Suppresses Innate Immunity
He, Xiang1,2; Jiang, He-wei1; Chen, Hong1; Zhang, Hai-nan1; Liu, Yin1; Xu, Zhao-wei1; Wu, Fan-lin1; Guo, Shu-juan1; Hou, Jing-li3; Yang, Ming-kun4; Yan, Wei1; Deng, Jiao-yu5; Bi, Li-jun6,7,8; Zhang, Xian-en6,7; Tao, Sheng-ce1,2,9
Corresponding AuthorTao, Sheng-ce(taosc@sjtu.edu.cn)
2017-12-01
Source PublicationMOLECULAR & CELLULAR PROTEOMICS
ISSN1535-9476
Volume16Issue:12Pages:2243-2253
AbstractMycobacterium tuberculosis (Mtb) has evolved multiple strategies to counter the human immune system. The effectors of Mtb play important roles in the interactions with the host. However, because of the lack of highly efficient strategies, there are only a handful of known Mtb effectors, thus hampering our understanding of Mtb pathogenesis. In this study, we probed Mtb proteome microarray with biotinylated whole-cell lysates of human macrophages, identifying 26 Mtb membrane proteins and secreted proteins that bind to macrophage proteins. Combining GST pull-down with mass spectroscopy then enabled the specific identification of all binders. We refer to this proteome microarray-based strategy as SOPHIE (Systematic unlOcking of Pathogen and Host Interacting Effectors). Detailed investigation of a novel effector identified here, the iron storage protein BfrB (Rv3841), revealed that BfrB inhibits NF-kappa B-dependent transcription through binding and reducing the nuclear abundance of the ribosomal protein S3 (RPS3), which is a functional subunit of NF-kappa B. The importance of this interaction was evidenced by the promotion of survival in macrophages of the mycobacteria, Mycobacterium smegmatis, by overexpression of BfrB. Thus, beyond demonstrating the power of SOPHIE in the discovery of novel effectors of human pathogens, we expect that the set of Mtb effectors identified in this work will greatly facilitate the understanding of the pathogenesis of Mtb, possibly leading to additional potential molecular targets in the battle against tuberculosis.
DOI10.1074/mcp.RA117.000296
Funding OrganizationNational Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Science and Technology Planning Projects of Guangdong Province ; Science and Technology Planning Projects of Guangdong Province ; Science and Technology Planning Projects of Guangdong Province ; Science and Technology Planning Projects of Guangdong Province ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Science and Technology Planning Projects of Guangdong Province ; Science and Technology Planning Projects of Guangdong Province ; Science and Technology Planning Projects of Guangdong Province ; Science and Technology Planning Projects of Guangdong Province ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Science and Technology Planning Projects of Guangdong Province ; Science and Technology Planning Projects of Guangdong Province ; Science and Technology Planning Projects of Guangdong Province ; Science and Technology Planning Projects of Guangdong Province ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Science and Technology Planning Projects of Guangdong Province ; Science and Technology Planning Projects of Guangdong Province ; Science and Technology Planning Projects of Guangdong Province ; Science and Technology Planning Projects of Guangdong Province ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences
Indexed BySCI ; SCI
Language英语
Funding ProjectNational Key Research and Development Program of China[2016YFA0500600] ; National Natural Science Foundation of China[31670831] ; National Natural Science Foundation of China[31370813] ; National Natural Science Foundation of China[31471232] ; Science and Technology Planning Projects of Guangdong Province[2014B030301040] ; Chinese Academy of Sciences[KJZD-EW-TZ-L04]
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemical Research Methods
WOS IDWOS:000417537800015
WOS KeywordPROTEIN-INTERACTION MAP ; DRUG DEVELOPMENT ; BACTERIAL PROTEINS ; BINDING PROTEINS ; SIGNAL PEPTIDES ; MACROPHAGES ; INFECTION ; TARGET ; MICROARRAY ; PTPA
PublisherAMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Funding OrganizationNational Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Science and Technology Planning Projects of Guangdong Province ; Science and Technology Planning Projects of Guangdong Province ; Science and Technology Planning Projects of Guangdong Province ; Science and Technology Planning Projects of Guangdong Province ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Science and Technology Planning Projects of Guangdong Province ; Science and Technology Planning Projects of Guangdong Province ; Science and Technology Planning Projects of Guangdong Province ; Science and Technology Planning Projects of Guangdong Province ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Science and Technology Planning Projects of Guangdong Province ; Science and Technology Planning Projects of Guangdong Province ; Science and Technology Planning Projects of Guangdong Province ; Science and Technology Planning Projects of Guangdong Province ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Science and Technology Planning Projects of Guangdong Province ; Science and Technology Planning Projects of Guangdong Province ; Science and Technology Planning Projects of Guangdong Province ; Science and Technology Planning Projects of Guangdong Province ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/30106
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorTao, Sheng-ce
Affiliation1.Shanghai Jiao Tong Univ, Minist Educ, Key Lab Syst Biomed, Shanghai Ctr Syst Biomed, Shanghai 200240, Peoples R China
2.Shanghai Jiao Tong Univ, Sch Biomed Engn, Shanghai 200240, Peoples R China
3.Shanghai Jiao Tong Univ, Instrumental Anal Ctr, Shanghai 200240, Peoples R China
4.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China
5.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China
6.Chinese Acad Sci, Inst Biophys, Natl Key Lab Biomacromol, Key Lab Noncoding RNA, Beijing 100101, Peoples R China
7.Chinese Acad Sci, Inst Biophys, Key Lab Prot & Peptide Pharmaceut, Beijing 100101, Peoples R China
8.Foshan Univ, Sch Stomatol & Med, Foshan 528000, Guangdong, Peoples R China
9.State Key Lab Oncogenes & Related Genes, Shanghai 200240, Peoples R China
Recommended Citation
GB/T 7714
He, Xiang,Jiang, He-wei,Chen, Hong,et al. Systematic Identification of Mycobacterium tuberculosis Effectors Reveals that BfrB Suppresses Innate Immunity[J]. MOLECULAR & CELLULAR PROTEOMICS,2017,16(12):2243-2253.
APA He, Xiang.,Jiang, He-wei.,Chen, Hong.,Zhang, Hai-nan.,Liu, Yin.,...&Tao, Sheng-ce.(2017).Systematic Identification of Mycobacterium tuberculosis Effectors Reveals that BfrB Suppresses Innate Immunity.MOLECULAR & CELLULAR PROTEOMICS,16(12),2243-2253.
MLA He, Xiang,et al."Systematic Identification of Mycobacterium tuberculosis Effectors Reveals that BfrB Suppresses Innate Immunity".MOLECULAR & CELLULAR PROTEOMICS 16.12(2017):2243-2253.
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