IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Alternative Splicing Transcripts of Zebrafish LGP2 Gene Differentially Contribute to IFN Antiviral Response
Zhang, Qi-Min1,2; Zhao, Xiang1,2; Li, Zhi1; Wu, Min1,2; Gui, Jian-Fang1,2; Zhang, Yi-Bing1,2
2018-01-15
Source PublicationJOURNAL OF IMMUNOLOGY
ISSN0022-1767
Volume200Issue:2Pages:688-703
Abstract

In mammals, RIG-I like receptors (RLRs) RIG-I and melanoma differentiation-associated gene 5 (MDA5) sense cytosolic viral RNA, leading to IFN antiviral response; however, LGP2 exhibits controversial functions. The same happens to fish LGP2. In this study we report that three zebrafish LGP2 splicing transcripts, a full-length LGP2, and two truncating variants, LGP2v1 and LGP2v2, play distinct roles during IFN antiviral response. Overexpression of the full-length LGP2 not only potentiates IFN response through the RLR pathway, in the absence or presence of poly(I:C) at limited concentrations, but also inhibits IFN response by relative high concentrations of poly(I: C) through functional attenuation of signaling factors involved in the RLR pathway; however, LGP2v1 and LGP2v2 only retain the inhibitory role. Consistently, LGP2 but not LGP2v1 and LGP2v2 confers protection on fish cells against spring viremia of carp virus (SVCV) infection and at limited expression levels, LGP2 exerts more significant protection than either RIG-I or MDA5. Further data suggest that in the early phase of SVCV infection, LGP2 functions as a positive regulator but along with SVCV replicating in cells up to a certain titer, which leads to a far more robust expression of IFN, LGP2 switches to a negative role. These in vitro results suggest an ingenious mechanism where the three zebrafish LGP2 splicing transcripts work cooperatively to shape IFN antiviral responses.

DOI10.4049/jimmunol.1701388
Funding OrganizationChinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory
Indexed BySCI
Language英语
Funding ProjectChinese Academy of Sciences[XDA08010207] ; National Natural Science Foundation[31572646] ; National Natural Science Foundation[31772875] ; Freshwater Ecology and Biotechnology Laboratory[2016FBZ01]
WOS Research AreaImmunology
WOS SubjectImmunology
WOS IDWOS:000419443900033
WOS KeywordDOUBLE-STRANDED-RNA ; INNATE IMMUNE-RESPONSE ; RIG-I ; HELICASE LGP2 ; FUNCTIONAL-CHARACTERIZATION ; ENDOPLASMIC-RETICULUM ; SIGNAL-TRANSDUCTION ; FISH ; VIRUS ; MDA5
PublisherAMER ASSOC IMMUNOLOGISTS
Funding OrganizationChinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory ; Freshwater Ecology and Biotechnology Laboratory
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/30090
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorZhang, Yi-Bing
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Key Lab Aquaculture Dis Control,Minist Agr, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 10049, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Qi-Min,Zhao, Xiang,Li, Zhi,et al. Alternative Splicing Transcripts of Zebrafish LGP2 Gene Differentially Contribute to IFN Antiviral Response[J]. JOURNAL OF IMMUNOLOGY,2018,200(2):688-703.
APA Zhang, Qi-Min,Zhao, Xiang,Li, Zhi,Wu, Min,Gui, Jian-Fang,&Zhang, Yi-Bing.(2018).Alternative Splicing Transcripts of Zebrafish LGP2 Gene Differentially Contribute to IFN Antiviral Response.JOURNAL OF IMMUNOLOGY,200(2),688-703.
MLA Zhang, Qi-Min,et al."Alternative Splicing Transcripts of Zebrafish LGP2 Gene Differentially Contribute to IFN Antiviral Response".JOURNAL OF IMMUNOLOGY 200.2(2018):688-703.
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