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Functional characterization of interferon regulatory factor 5 and its role in the innate antiviral immune response
Ai, Kete1,2; Luo, Kai1; Xia, Lihai1; Gao, Weihua1; Hu, Wei1,3; Qi, Zhitao1,3; Xu, Qiaoqing1,3
2018
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume72Issue:1Pages:31-36
Abstract

In mammals, type I interferons (IFNs) are primarily regulated by transcription factors of the IFN regulatory (IRE) family. Interferon regulatory factor 5 (IRF-5) plays pivotal roles in antiviral and inflammatory responses. In the present study, we found that zebrafish (Danio rerio) IRF5 is a key player in the regulation of the expression of type I IFN and its antiviral immune response. IRF5 was upregulated in zebrafish embryonic fibroblast cells (ZF4) when challenged with grass carp reovirus (GCRV). Moreover, the expression profiles of Mx, IFN, Viperin, and IRF7, but not IRF3, were upregulated by overexpression of IRF5 in Epithelioma papulosum cyprinid cells (EPCs). Luciferase assays revealed that the activation of the IFN phi 1 promoter was stimulated by overexpression of IRF5 and IRF5-Delta IAD (IRF5 lacking the IRF-associated domain), respectively. However, overexpression of IRF5 or IRF5-Delta IAD inhibited the activity of the IFN phi 3 promoter. IRF5-Delta DBD (lacking the DNA-binding domain) had no influence in the activation of the IFN phi 1 and IFN phi 3 promoters. Furthermore, the determination of the cytopathic effect (CPE) numbers and viral titers revealed that the viral concentration was reduced by ectopic expression of IRF5 in EPC cells. Ectopic expression of IRF5 in EPC cells could protect cells from GCRV and significantly inhibited GCRV virus replication. These data indicated that IRF5 could limit viral replication through an IFN-dependent pathway.

KeywordZebrafish IRF5 Antiviral immune response
DOI10.1016/j.fsi.2017.10.042
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31101928] ; Engineering Research Center of Ecology and Agricultural Use of Wetland[KF201608] ; Engineering Research Center of Ecology and Agricultural Use of Wetland[10201611]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000423644600005
WOS KeywordEXPRESSION ANALYSIS ; MOLECULAR CHARACTERIZATION ; CTENOPHARYNGODON-IDELLA ; TRANSCRIPTION FACTORS ; ZEBRAFISH IRF1 ; GRASS CARP ; ACTIVATION ; GENES ; FAMILY ; LOCALIZATION
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland ; Engineering Research Center of Ecology and Agricultural Use of Wetland
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/29988
Collection其他_期刊论文
Corresponding AuthorXu, Qiaoqing
Affiliation1.Minist Educ, Engn Res Ctr Ecol & Agr Use Wetland, Jingzhou 434020, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
3.Yangtze Univ, Sch Anim Sci, Jingzhou 434020, Peoples R China
Recommended Citation
GB/T 7714
Ai, Kete,Luo, Kai,Xia, Lihai,et al. Functional characterization of interferon regulatory factor 5 and its role in the innate antiviral immune response[J]. FISH & SHELLFISH IMMUNOLOGY,2018,72(1):31-36.
APA Ai, Kete.,Luo, Kai.,Xia, Lihai.,Gao, Weihua.,Hu, Wei.,...&Xu, Qiaoqing.(2018).Functional characterization of interferon regulatory factor 5 and its role in the innate antiviral immune response.FISH & SHELLFISH IMMUNOLOGY,72(1),31-36.
MLA Ai, Kete,et al."Functional characterization of interferon regulatory factor 5 and its role in the innate antiviral immune response".FISH & SHELLFISH IMMUNOLOGY 72.1(2018):31-36.
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