IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Mettl3 Mutation Disrupts Gamete Maturation and Reduces Fertility in Zebrafish
Xia, Hui1,2; Zhong, Chengrong1; Wu, Xingxing1,2; Chen, Ji1; Tao, Binbin1,2; Xia, Xiaoqin1; Shi, Mijuan1; Zhu, Zuoyan1; Trudeau, Vance L.3; Hu, Wei1
2018-02-01
Source PublicationGENETICS
ISSN0016-6731
Volume208Issue:2Pages:729-743
Abstract

N-6-methyladenosine (m(6)A), catalyzed by Mettl3 methyltransferase, is a highly conserved epigenetic modification in eukaryotic messenger RNA (mRNA). Previous studies have implicated m(6)A modification in multiple biological processes, but the in vivo function of m(6)A has been difficult to study, because mettl3 mutants are embryonic lethal in both mammals and plants. In this study, we have used transcription activator-like effector nucleases and generated viable zygotic mettl3 mutant, Zmettl3(m/m), in zebrafish. We find that the oocytes in Zmettl3(m/m) adult females are stalled in early development and the ratio of full-grown stage (FG) follicles is significantly lower than that of wild type. Human chorionic gonadotropin-induced ovarian germinal vesicle breakdown in vitro and the numbers of eggs ovulated in vivo are both decreased as well, while the defects of oocyte maturation can be rescued by sex hormone in vitro and in vivo. In Zmettl3(m/m) adult males, we find defects in sperm maturation and sperm motility is significantly reduced. Further study shows that 11-ketotestosterone (11-KT) and 17-estradiol (E2) levels are significantly decreased in Zmettl3(m/m), and defective gamete maturation is accompanied by decreased overall m(6)A modification levels and disrupted expression of genes critical for sex hormone synthesis and gonadotropin signaling in Zmettl3(m/m). Thus, our study provides the first in vivo evidence that loss of Mettl3 leads to failed gamete maturation and significantly reduced fertility in zebrafish. Mettl3 and m(6)A modifications are essential for optimal reproduction in vertebrates.

Keywordmettl3 N-6-methyladenosine gamete maturation fertility zebrafish
DOI10.1534/genetics.117.300574
Funding OrganizationNational Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation[31325026] ; National Natural Science Foundation[31721005] ; National Natural Science Foundation[31461163006] ; Chinese Academy of Sciences[XDA08010106] ; Chinese Academy of Sciences[2016FBZ03] ; University of Ottawa International Research Acceleration Program
WOS Research AreaGenetics & Heredity
WOS SubjectGenetics & Heredity
WOS IDWOS:000423563400020
WOS KeywordMESSENGER-RNA METHYLATION ; GENE-EXPRESSION REGULATION ; MEDAKA ORYZIAS-LATIPES ; EMBRYONIC STEM-CELLS ; OOCYTE MATURATION ; SEX DETERMINATION ; NUCLEAR-RNA ; STEREOLOGICAL EVALUATION ; BRACHYDANIO-RERIO ; DANIO-RERIO
PublisherGENETICS SOCIETY AMERICA
Funding OrganizationNational Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/29945
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorHu, Wei
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, 7 Donghu South Rd, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Univ Ottawa, Dept Biol, Ottawa, ON K1N 6N5, Canada
Recommended Citation
GB/T 7714
Xia, Hui,Zhong, Chengrong,Wu, Xingxing,et al. Mettl3 Mutation Disrupts Gamete Maturation and Reduces Fertility in Zebrafish[J]. GENETICS,2018,208(2):729-743.
APA Xia, Hui.,Zhong, Chengrong.,Wu, Xingxing.,Chen, Ji.,Tao, Binbin.,...&Hu, Wei.(2018).Mettl3 Mutation Disrupts Gamete Maturation and Reduces Fertility in Zebrafish.GENETICS,208(2),729-743.
MLA Xia, Hui,et al."Mettl3 Mutation Disrupts Gamete Maturation and Reduces Fertility in Zebrafish".GENETICS 208.2(2018):729-743.
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