Mettl3 Mutation Disrupts Gamete Maturation and Reduces Fertility in Zebrafish | |
Xia, Hui1,2; Zhong, Chengrong1; Wu, Xingxing1,2; Chen, Ji1; Tao, Binbin1,2; Xia, Xiaoqin1; Shi, Mijuan1; Zhu, Zuoyan1; Trudeau, Vance L.3; Hu, Wei1 | |
2018-02-01 | |
Source Publication | GENETICS
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ISSN | 0016-6731 |
Volume | 208Issue:2Pages:729-743 |
Abstract | N-6-methyladenosine (m(6)A), catalyzed by Mettl3 methyltransferase, is a highly conserved epigenetic modification in eukaryotic messenger RNA (mRNA). Previous studies have implicated m(6)A modification in multiple biological processes, but the in vivo function of m(6)A has been difficult to study, because mettl3 mutants are embryonic lethal in both mammals and plants. In this study, we have used transcription activator-like effector nucleases and generated viable zygotic mettl3 mutant, Zmettl3(m/m), in zebrafish. We find that the oocytes in Zmettl3(m/m) adult females are stalled in early development and the ratio of full-grown stage (FG) follicles is significantly lower than that of wild type. Human chorionic gonadotropin-induced ovarian germinal vesicle breakdown in vitro and the numbers of eggs ovulated in vivo are both decreased as well, while the defects of oocyte maturation can be rescued by sex hormone in vitro and in vivo. In Zmettl3(m/m) adult males, we find defects in sperm maturation and sperm motility is significantly reduced. Further study shows that 11-ketotestosterone (11-KT) and 17-estradiol (E2) levels are significantly decreased in Zmettl3(m/m), and defective gamete maturation is accompanied by decreased overall m(6)A modification levels and disrupted expression of genes critical for sex hormone synthesis and gonadotropin signaling in Zmettl3(m/m). Thus, our study provides the first in vivo evidence that loss of Mettl3 leads to failed gamete maturation and significantly reduced fertility in zebrafish. Mettl3 and m(6)A modifications are essential for optimal reproduction in vertebrates. |
Keyword | mettl3 N-6-methyladenosine gamete maturation fertility zebrafish |
DOI | 10.1534/genetics.117.300574 |
Funding Organization | National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program |
Indexed By | SCI |
Language | 英语 |
Funding Project | National Natural Science Foundation[31325026] ; National Natural Science Foundation[31721005] ; National Natural Science Foundation[31461163006] ; Chinese Academy of Sciences[XDA08010106] ; Chinese Academy of Sciences[2016FBZ03] ; University of Ottawa International Research Acceleration Program |
WOS Research Area | Genetics & Heredity |
WOS Subject | Genetics & Heredity |
WOS ID | WOS:000423563400020 |
WOS Keyword | MESSENGER-RNA METHYLATION ; GENE-EXPRESSION REGULATION ; MEDAKA ORYZIAS-LATIPES ; EMBRYONIC STEM-CELLS ; OOCYTE MATURATION ; SEX DETERMINATION ; NUCLEAR-RNA ; STEREOLOGICAL EVALUATION ; BRACHYDANIO-RERIO ; DANIO-RERIO |
Publisher | GENETICS SOCIETY AMERICA |
Funding Organization | National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; National Natural Science Foundation ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program ; University of Ottawa International Research Acceleration Program |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.ihb.ac.cn/handle/342005/29945 |
Collection | 鱼类生物学及渔业生物技术研究中心_期刊论文 |
Corresponding Author | Hu, Wei |
Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, 7 Donghu South Rd, Wuhan 430072, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Univ Ottawa, Dept Biol, Ottawa, ON K1N 6N5, Canada |
Recommended Citation GB/T 7714 | Xia, Hui,Zhong, Chengrong,Wu, Xingxing,et al. Mettl3 Mutation Disrupts Gamete Maturation and Reduces Fertility in Zebrafish[J]. GENETICS,2018,208(2):729-743. |
APA | Xia, Hui.,Zhong, Chengrong.,Wu, Xingxing.,Chen, Ji.,Tao, Binbin.,...&Hu, Wei.(2018).Mettl3 Mutation Disrupts Gamete Maturation and Reduces Fertility in Zebrafish.GENETICS,208(2),729-743. |
MLA | Xia, Hui,et al."Mettl3 Mutation Disrupts Gamete Maturation and Reduces Fertility in Zebrafish".GENETICS 208.2(2018):729-743. |
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