IHB OpenIR  > 藻类生物学及应用研究中心  > 期刊论文
Acetylome Profiling Reveals Extensive Lysine Acetylation of the Fatty Acid Metabolism Pathway in the Diatom Phaeodactylum tricornutum
Chen, Zhuo1,2; Luo, Ling1; Chen, Runfa1; Hu, Hanhua3; Pan, Yufang3; Jiang, Haibo4,5; Wan, Xia1; Jin, Hu3; Gong, Yangmin1
2018-03-01
Source PublicationMOLECULAR & CELLULAR PROTEOMICS
ISSN1535-9476
Volume17Issue:3Pages:399-412
Abstract

N-epsilon-lysine acetylation represents a highly dynamic and reversibly regulated post-translational modification widespread in almost all organisms, and plays important roles for regulation of protein function in diverse metabolic pathways. However, little is known about the role of lysine acetylation in photosynthetic eukaryotic microalgae. We integrated proteomic approaches to comprehensively characterize the lysine acetylome in the model diatom Phaeodactylum tricornutum. In total, 2324 acetylation sites from 1220 acetylated proteins were identified, representing the largest data set of the lysine acetylome in plants to date. Almost all enzymes involved in fatty acid synthesis were found to be lysine acetylated. Six putative lysine acetylation sites were identified in a plastid-localized long-chain acyl-CoA synthetase. Site-directed mutagenesis and site-specific incorporation of N-acetyllysine in acyl-CoA synthetase show that acetylation at K407 and K425 increases its enzyme activity. Moreover, the nonenzymatically catalyzed overall hyperacetylation of acyl-CoA synthetase by acetyl-phosphate can be effectively deacetylated and reversed by a sirtuin-type NAD(+)-dependent deacetylase with subcellular localization of both the plastid and nucleus in Phaeodactylum. This work indicates the regulation of acyl-CoA synthetase activity by site-specific lysine acetylation and highlights the potential regulation of fatty acid metabolism by lysine actetylation in the plastid of the diatom Phaeodactylum.

DOI10.1074/mcp.RA117.000339
Funding OrganizationAgricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
Indexed BySCI
Language英语
Funding ProjectAgricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences[CAAS-ASTIP-2013-OCRI] ; National Natural Science Foundation of China[31370746]
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemical Research Methods
WOS IDWOS:000426370400003
WOS KeywordCHAIN ACYL-COENZYME ; RHODOPSEUDOMONAS-PALUSTRIS ; PROTEIN-PHOSPHORYLATION ; FUNCTIONAL GENOMICS ; SALMONELLA-ENTERICA ; COA SYNTHETASE ; PCC 7002 ; IDENTIFICATION ; ARABIDOPSIS ; PHOSPHOPROTEOME
PublisherAMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Funding OrganizationAgricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/29852
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorGong, Yangmin
Affiliation1.Chinese Acad Agr Sci, Minist Agr, Oil Crops Res Inst, Key Lab Biol & Genet Improvement Oil Crops, Wuhan 430062, Hubei, Peoples R China
2.Shandong Normal Univ, Coll Life Sci, Key Lab Plant Stress Res, Jinan 250014, Shandong, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China
4.Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China
5.Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Chen, Zhuo,Luo, Ling,Chen, Runfa,et al. Acetylome Profiling Reveals Extensive Lysine Acetylation of the Fatty Acid Metabolism Pathway in the Diatom Phaeodactylum tricornutum[J]. MOLECULAR & CELLULAR PROTEOMICS,2018,17(3):399-412.
APA Chen, Zhuo.,Luo, Ling.,Chen, Runfa.,Hu, Hanhua.,Pan, Yufang.,...&Gong, Yangmin.(2018).Acetylome Profiling Reveals Extensive Lysine Acetylation of the Fatty Acid Metabolism Pathway in the Diatom Phaeodactylum tricornutum.MOLECULAR & CELLULAR PROTEOMICS,17(3),399-412.
MLA Chen, Zhuo,et al."Acetylome Profiling Reveals Extensive Lysine Acetylation of the Fatty Acid Metabolism Pathway in the Diatom Phaeodactylum tricornutum".MOLECULAR & CELLULAR PROTEOMICS 17.3(2018):399-412.
Files in This Item: Download All
File Name/Size DocType Version Access License
399.full.pdf(2068KB)期刊论文出版稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Chen, Zhuo]'s Articles
[Luo, Ling]'s Articles
[Chen, Runfa]'s Articles
Baidu academic
Similar articles in Baidu academic
[Chen, Zhuo]'s Articles
[Luo, Ling]'s Articles
[Chen, Runfa]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Chen, Zhuo]'s Articles
[Luo, Ling]'s Articles
[Chen, Runfa]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 399.full.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.