Acetylome Profiling Reveals Extensive Lysine Acetylation of the Fatty Acid Metabolism Pathway in the Diatom Phaeodactylum tricornutum | |
Chen, Zhuo1,2; Luo, Ling1; Chen, Runfa1; Hu, Hanhua3; Pan, Yufang3; Jiang, Haibo4,5; Wan, Xia1; Jin, Hu3; Gong, Yangmin1 | |
2018-03-01 | |
Source Publication | MOLECULAR & CELLULAR PROTEOMICS
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ISSN | 1535-9476 |
Volume | 17Issue:3Pages:399-412 |
Abstract | N-epsilon-lysine acetylation represents a highly dynamic and reversibly regulated post-translational modification widespread in almost all organisms, and plays important roles for regulation of protein function in diverse metabolic pathways. However, little is known about the role of lysine acetylation in photosynthetic eukaryotic microalgae. We integrated proteomic approaches to comprehensively characterize the lysine acetylome in the model diatom Phaeodactylum tricornutum. In total, 2324 acetylation sites from 1220 acetylated proteins were identified, representing the largest data set of the lysine acetylome in plants to date. Almost all enzymes involved in fatty acid synthesis were found to be lysine acetylated. Six putative lysine acetylation sites were identified in a plastid-localized long-chain acyl-CoA synthetase. Site-directed mutagenesis and site-specific incorporation of N-acetyllysine in acyl-CoA synthetase show that acetylation at K407 and K425 increases its enzyme activity. Moreover, the nonenzymatically catalyzed overall hyperacetylation of acyl-CoA synthetase by acetyl-phosphate can be effectively deacetylated and reversed by a sirtuin-type NAD(+)-dependent deacetylase with subcellular localization of both the plastid and nucleus in Phaeodactylum. This work indicates the regulation of acyl-CoA synthetase activity by site-specific lysine acetylation and highlights the potential regulation of fatty acid metabolism by lysine actetylation in the plastid of the diatom Phaeodactylum. |
DOI | 10.1074/mcp.RA117.000339 |
Funding Organization | Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China |
Indexed By | SCI |
Language | 英语 |
Funding Project | Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences[CAAS-ASTIP-2013-OCRI] ; National Natural Science Foundation of China[31370746] |
WOS Research Area | Biochemistry & Molecular Biology |
WOS Subject | Biochemical Research Methods |
WOS ID | WOS:000426370400003 |
WOS Keyword | CHAIN ACYL-COENZYME ; RHODOPSEUDOMONAS-PALUSTRIS ; PROTEIN-PHOSPHORYLATION ; FUNCTIONAL GENOMICS ; SALMONELLA-ENTERICA ; COA SYNTHETASE ; PCC 7002 ; IDENTIFICATION ; ARABIDOPSIS ; PHOSPHOPROTEOME |
Publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC |
Funding Organization | Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.ihb.ac.cn/handle/342005/29852 |
Collection | 藻类生物学及应用研究中心_期刊论文 |
Corresponding Author | Gong, Yangmin |
Affiliation | 1.Chinese Acad Agr Sci, Minist Agr, Oil Crops Res Inst, Key Lab Biol & Genet Improvement Oil Crops, Wuhan 430062, Hubei, Peoples R China 2.Shandong Normal Univ, Coll Life Sci, Key Lab Plant Stress Res, Jinan 250014, Shandong, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China 4.Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China 5.Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China |
Recommended Citation GB/T 7714 | Chen, Zhuo,Luo, Ling,Chen, Runfa,et al. Acetylome Profiling Reveals Extensive Lysine Acetylation of the Fatty Acid Metabolism Pathway in the Diatom Phaeodactylum tricornutum[J]. MOLECULAR & CELLULAR PROTEOMICS,2018,17(3):399-412. |
APA | Chen, Zhuo.,Luo, Ling.,Chen, Runfa.,Hu, Hanhua.,Pan, Yufang.,...&Gong, Yangmin.(2018).Acetylome Profiling Reveals Extensive Lysine Acetylation of the Fatty Acid Metabolism Pathway in the Diatom Phaeodactylum tricornutum.MOLECULAR & CELLULAR PROTEOMICS,17(3),399-412. |
MLA | Chen, Zhuo,et al."Acetylome Profiling Reveals Extensive Lysine Acetylation of the Fatty Acid Metabolism Pathway in the Diatom Phaeodactylum tricornutum".MOLECULAR & CELLULAR PROTEOMICS 17.3(2018):399-412. |
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