IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Dietary stachyose altered the intestinal microbiota profile and improved the intestinal mucosal barrier function of juvenile turbot, Scophthalmus maximus L.
Yang, Pei1,2; Hu, Haibin1,2; Liu, Yang1,2; Li, Yanxian1,2; Ai, Qinghui1,2; Xu, Wei1,2; Zhang, Wenbing1,2; Zhang, Yongan3; Zhang, Yanjiao1,2; Mai, Kangsen1,2
2018-02-03
Source PublicationAQUACULTURE
ISSN0044-8486
Volume486Issue:1Pages:98-106
Abstract

Recent studies have revealed the beneficial effects of stachyose on intestinal histology and digestive function of fish. However, a comprehensive understanding of stachyose's impact on intestinal health of fish remains unclear, limiting its use in aqua-feed. In the present study, a 12-week feeding trial was conducted to investigate the effects of dietary stachyose on intestinal microbiota and mucosal barrier function of turbot (S. maximus L). Three isonitrogenous and isolipidic experimental diets were formulated to contain 0%, 1.25% and 5% stachyose, respectively. Sequencing of bacterial 16s rRNA V-4 region indicated that dietary stachyose altered the intestinal adherent microbiota profile, which was supported by the diet-cluster of PCA, PCoA, beta diversity heatmap and phylogenetic tree. LEfSe and MetaStat analysis indicated that both 1.25% and 5% dietary stachyose significantly elevated the abundance of intestinal cellulose-degrading bacteria. However, the higher level of stachyose (5%) increased the abundance of intestinal beneficial bacteria as well as that of potential pathogenic bacteria. Moreover, 1.25% dietary stachyose significantly up-regulated the genes expression of occludin, claudin-3, and ZO-1, and down-regulated the gene expression of claudin-like in the intestine (P<0.05). Dietary stachyose at 5% significantly increased mucin-2 secretion and the gene expression of ZO-1, while significantly decreased the gene expression of claudin-like in the intestine (P<0.05). Collectively, our study showed that dietary stachyose supplementation could favorably modulate the profile of intestinal microbiota and enhance the intestinal mucosal barrier function in juvenile turbot. Stachyose showed promising potential of being used as prebiotic in diet for enhancing the intestinal health of turbot.

KeywordStachyose Intestinal microbiota Intestinal tight junction Turbot
DOI10.1016/j.aquaculture.2017.12.014
Funding OrganizationNational Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System
Indexed BySCI
Language英语
Funding ProjectNational Basic Research Program (973 Program)[2014CB138601] ; National Natural Science Foundation of China[41576137] ; National Natural Science Foundation of China[31202006] ; China Agriculture Researches System[CARS 50-G08]
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000425875900014
WOS KeywordSALMON SALMO-SALAR ; ALPHA-GALACTO-OLIGOSACCHARIDES ; CARP CTENOPHARYNGODON-IDELLA ; DIGESTIVE ENZYME-ACTIVITIES ; MUCIN GENE-EXPRESSION ; GUT MICROBIOTA ; ATLANTIC SALMON ; GROWTH-PERFORMANCE ; FISH ; BACTERIA
PublisherELSEVIER SCIENCE BV
Funding OrganizationNational Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Basic Research Program (973 Program) ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System ; China Agriculture Researches System
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/29849
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorZhang, Yanjiao
Affiliation1.Ocean Univ China, Minist Agr, Key Lab Aquaculture Nutr & Feed, Qingdao, Shandong, Peoples R China
2.Ocean Univ China, Minist Agr, Key Lab Mariculture, Qingdao, Shandong, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Yang, Pei,Hu, Haibin,Liu, Yang,et al. Dietary stachyose altered the intestinal microbiota profile and improved the intestinal mucosal barrier function of juvenile turbot, Scophthalmus maximus L.[J]. AQUACULTURE,2018,486(1):98-106.
APA Yang, Pei.,Hu, Haibin.,Liu, Yang.,Li, Yanxian.,Ai, Qinghui.,...&Mai, Kangsen.(2018).Dietary stachyose altered the intestinal microbiota profile and improved the intestinal mucosal barrier function of juvenile turbot, Scophthalmus maximus L..AQUACULTURE,486(1),98-106.
MLA Yang, Pei,et al."Dietary stachyose altered the intestinal microbiota profile and improved the intestinal mucosal barrier function of juvenile turbot, Scophthalmus maximus L.".AQUACULTURE 486.1(2018):98-106.
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