IHB OpenIR  > 藻类生物学及应用研究中心  > 期刊论文
N-Glycoproteomic Characterization of Mannosidase and Xylosyltransferase Mutant Strains of Chlamydomonas reinhardtii
Schulze, Stefan1,4; Oltmanns, Anne1; Machnik, Nick1; Liu, Gai2; Xu, Nannan2,3; Jarmatz, Niklas1; Scholz, Martin1; Sugimoto, Kazuhiko1; Fufezan, Christian1; Huang, Kaiyao2; Hippler, Michael1
2018-03-01
Source PublicationPLANT PHYSIOLOGY
ISSN0032-0889
Volume176Issue:3Pages:1952-1964
Abstract

At present, only little is known about the enzymatic machinery required for N-glycosylation in Chlamydomonas reinhardtii, leading to the formation of N-glycans harboring Xyl and methylated Man. This machinery possesses new enzymatic features, as C. reinhardtii N-glycans are independent of beta 1,2-N-acetylglucosaminyltransferase I. Here we have performed comparative N-glycoproteomic analyses of insertional mutants of mannosidase 1A (IMMan1A) and xylosyltransferase 1A (IMXylT1A). The disruption of man1A affected methylation of Man and the addition of terminal Xyl. The absence of XylT1A led to shorter Nglycans compared to the wild type. The use of a IM(Man1A)xIM(XylT1A) double mutant revealed that the absence of Man1A suppressed the IMXylT1A phenotype, indicating that the increased N-glycan trimming is regulated by core beta 1,2-Xyl and is dependent on Man1A activity. These data point toward an enzymatic cascade in the N-glycosylation pathway of C. reinhardtii with interlinked roles of Man1A and XylT1A. The results described herein represent the first step toward a functional characterization of the enzymatic N-glycosylation machinery in C. reinhardtii.

DOI10.1104/pp.17.01450
Funding OrganizationDeutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
Indexed BySCI
Language英语
Funding ProjectDeutsche Forschungsgemeinschaft[HI 739/12-1] ; Sino-German Center, Beijing, China[GZ990] ; National Natural Science Foundation of China[31371354] ; National Natural Science Foundation of China[31400654]
WOS Research AreaPlant Sciences
WOS SubjectPlant Sciences
WOS IDWOS:000426848300012
WOS KeywordMASS-SPECTROMETRY DATA ; ARABIDOPSIS-THALIANA ; GLYCOSYLATION PATHWAY ; PYTHON MODULE ; GLYCANS ; UNIVERSAL ; DATABASE ; SEARCH ; GENERATION ; MATURATION
PublisherAMER SOC PLANT BIOLOGISTS
Funding OrganizationDeutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Deutsche Forschungsgemeinschaft ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; Sino-German Center, Beijing, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/29775
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorHippler, Michael
Affiliation1.Univ Munster, Inst Plant Biol & Biotechnol, D-48143 Munster, Germany
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
4.Univ Penn, Dept Biol, Philadelphia, PA 19104 USA
Recommended Citation
GB/T 7714
Schulze, Stefan,Oltmanns, Anne,Machnik, Nick,et al. N-Glycoproteomic Characterization of Mannosidase and Xylosyltransferase Mutant Strains of Chlamydomonas reinhardtii[J]. PLANT PHYSIOLOGY,2018,176(3):1952-1964.
APA Schulze, Stefan.,Oltmanns, Anne.,Machnik, Nick.,Liu, Gai.,Xu, Nannan.,...&Hippler, Michael.(2018).N-Glycoproteomic Characterization of Mannosidase and Xylosyltransferase Mutant Strains of Chlamydomonas reinhardtii.PLANT PHYSIOLOGY,176(3),1952-1964.
MLA Schulze, Stefan,et al."N-Glycoproteomic Characterization of Mannosidase and Xylosyltransferase Mutant Strains of Chlamydomonas reinhardtii".PLANT PHYSIOLOGY 176.3(2018):1952-1964.
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