Identification, expression analysis, and antibacterial activity of NK-lysin from common carp Cyprinus carpio | |
Wang, Gai Ling1; Wang, Ming Cheng1; Liu, Ying Li2; Zhang, Qian2; Li, Chuan Feng1; Liu, Pan Ting1; Li, En Zhong1; Nie, Pin2,3; Xie, Hai Xia2,3 | |
2018-02-01 | |
Source Publication | FISH & SHELLFISH IMMUNOLOGY
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ISSN | 1050-4648 |
Volume | 73Issue:1Pages:11-21 |
Abstract | Natural killer lysin (NK-lysin), produced by cytotoxic T lymphocytes and natural killer cells, is a cationic antimicrobial peptide that has a broad antimicrobial spectrum, including bacteria, viruses, and parasites. Nevertheless, the implication of NK-lysin in the protection against bacterial infection is not aware in common carp. In this study, six different NK-Iysin genes (nkl1, nkl2, nkI3, nkl4, nkl5 and nkl6) were identified in the common carp genome. Each of the mature peptides of common carp NK-lysin has six well-conserved cysteine residues, and shares a Saposin B domain, characteristic of saposin-like protein (SAID) family. The gene nkl1 contains 5 extrons and 4 introns, and nkl2, nkl3, nkl4 or nk15 contains 4 extrons and 3 introns, however, the nkl6 has 3 extrons and 2 introns. By quantitative real-time PCR, nkl2 transcripts were predominantly expressed in spleen of healthy common carp, while elevated mainly in gill and spleen upon Aeromonas hydrophila infection. The recombinant NK-lysin-2 purified from Pichia pastoris shows antibacterial activity against Staphylococcus aureus (Gram-positive), and Escherkhia coil M15, Aeromonas hydrophila, as well as Edwardsiella tarda (Gram-negative), the latter two are important pathogens of aquaculture. Our results indicate that NK-lysin in common carp might play an important role in fish immune response by enhancing antibacterial defense against bacterial pathogens. |
Keyword | NK-lysin Transcription analysis Protein purification Antibacterial activity Cyprinus carpio |
DOI | 10.1016/j.fsi.2017.11.030 |
Funding Organization | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province |
Indexed By | SCI |
Language | 英语 |
Funding Project | National Natural Science Foundation of China[31402334] ; National Natural Science Foundation of China[31572659] ; Basic and Frontier Technology Research Program of Henan Province[142300410111] |
WOS Research Area | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
WOS Subject | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
WOS ID | WOS:000424853100002 |
WOS Keyword | HOST-DEFENSE PEPTIDES ; ANTIMICROBIAL PEPTIDE ; CHANNEL CATFISH ; CANCER-CELLS ; GENE ; CHICKEN ; BINDING |
Publisher | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
Funding Organization | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province ; Basic and Frontier Technology Research Program of Henan Province |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.ihb.ac.cn/handle/342005/29769 |
Collection | 其他_期刊论文 |
Corresponding Author | Xie, Hai Xia |
Affiliation | 1.Huanghuai Univ, Coll Biol & Food Engn, Zhumadicm 463000, Henan, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 3.Minist Agr, Key Lab Aquaculture Dis Control, Wuhan 430072, Hubei, Peoples R China |
Recommended Citation GB/T 7714 | Wang, Gai Ling,Wang, Ming Cheng,Liu, Ying Li,et al. Identification, expression analysis, and antibacterial activity of NK-lysin from common carp Cyprinus carpio[J]. FISH & SHELLFISH IMMUNOLOGY,2018,73(1):11-21. |
APA | Wang, Gai Ling.,Wang, Ming Cheng.,Liu, Ying Li.,Zhang, Qian.,Li, Chuan Feng.,...&Xie, Hai Xia.(2018).Identification, expression analysis, and antibacterial activity of NK-lysin from common carp Cyprinus carpio.FISH & SHELLFISH IMMUNOLOGY,73(1),11-21. |
MLA | Wang, Gai Ling,et al."Identification, expression analysis, and antibacterial activity of NK-lysin from common carp Cyprinus carpio".FISH & SHELLFISH IMMUNOLOGY 73.1(2018):11-21. |
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