IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Transcriptomics Sequencing Provides Insights into Understanding the Mechanism of Grass Carp Reovirus Infection
Chen, Geng1,2; He, Libo1; Luo, Lifei1,2; Huang, Rong1; Liao, Lanjie1; Li, Yongming1; Zhu, Zuoyan1; Wang, Yaping
2018-02-01
Source PublicationINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN1422-0067
Volume19Issue:2Pages:16
Abstract

Grass carp is an important aquaculture fish species in China that is affected by severe diseases, especially haemorrhagic disease caused by grass carp reovirus (GCRV). However, the mechanisms of GCRV invasion and infection remain to be elucidated. In the present study, Ctenopharyngodon idellus kidney (CIK) cells were infected with GCRV, harvested at 0, 8, 24, and 72 h post infection, respectively, and then subjected to transcriptomics sequencing. Each sample yielded more than 6 Gb of clean data and 40 million clean reads. To better understand GCRV infection, the process was divided into three phases: the early (0-8 h post infection), middle (8-24 h post infection), and late (24-72 h) stages of infection. A total of 76 (35 up-regulated, 41 down-regulated), 553 (463 up-regulated, 90 down-regulated), and 284 (150 up-regulated, 134 down-regulated) differently expressed genes (DEGs) were identified during the early, middle, and late stages of infection, respectively. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed that DEGs were mainly involved in carbohydrate biosynthesis, transport, and endocytosis in the early stage, phagocytosis and lysosome pathways were mainly enriched in the middle stage, and programmed cell death, apoptosis, and inflammation were largely associated with the late stage. These results suggest GCRV infection is a gradual process involving adsorption on the cell surface, followed by endocytosis into cells, transport by lysosomes, and eventually resulted in cell necrosis and/or apoptosis. Our findings provide insight into the mechanisms of grass carp reovirus infection.

Keywordgrass carp grass carp reovirus transcriptomics sequencing phagosome
DOI10.3390/ijms19020488
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31721005] ; National Natural Science Foundation of China[31702332] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDA08030203] ; Hubei Provincial Natural Science Foundation of China[2015CFA007]
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS IDWOS:000427527400172
WOS KeywordSURFACE HEPARAN-SULFATE ; FOCAL ADHESION KINASE ; CTENOPHARYNGODON-IDELLUS ; SUBUNIT VACCINE ; VIRUS-INFECTION ; RNA-SEQ ; IMMUNITY ; AUTOIMMUNITY ; RECEPTORS ; ROLES
PublisherMDPI
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; 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National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/29732
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorChen, Geng
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 101408, Peoples R China
Recommended Citation
GB/T 7714
Chen, Geng,He, Libo,Luo, Lifei,et al. Transcriptomics Sequencing Provides Insights into Understanding the Mechanism of Grass Carp Reovirus Infection[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2018,19(2):16.
APA Chen, Geng.,He, Libo.,Luo, Lifei.,Huang, Rong.,Liao, Lanjie.,...&Wang, Yaping.(2018).Transcriptomics Sequencing Provides Insights into Understanding the Mechanism of Grass Carp Reovirus Infection.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,19(2),16.
MLA Chen, Geng,et al."Transcriptomics Sequencing Provides Insights into Understanding the Mechanism of Grass Carp Reovirus Infection".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 19.2(2018):16.
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