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RIP2 Is a Critical Regulator for NLRs Signaling and MHC Antigen Presentation but Not for MAPK and PI3K/Akt Pathways
Wu, Xiao Man1,2; Chen, Wen Qin3; Hu, Yi Wei1; Cao, Lu1; Nie, Pin1,4; Chang, Ming Xian1,4
Corresponding AuthorChang, Ming Xian(mingxianchang@ihb.ac.cn)
2018-04-10
Source PublicationFRONTIERS IN IMMUNOLOGY
ISSN1664-3224
Volume9Pages:18
AbstractRIP2 is an adaptor protein which is essential for the activation of NF-kappa B and NOD1- and NOD2-dependent signaling. Although NOD-RIP2 axis conservatively existed in the teleost, the function of RIP2 was only reported in zebrafish, goldfish, and rainbow trout in vitro. Very little is known about the role and mechanisms of piscine NOD-RIP2 axis in vivo. Our previous study showed the protective role of zebrafish NOD1 in larval survival through CD44a-mediated activation of PI3K-Akt signaling. In this study, we examined whether RIP2 was required for larval survival with or without pathogen infection, and determined the signaling pathways modulated by RIP2. Based on our previous report and the present study, our data demonstrated that NOD1-RIP2 axis was important for larval survival in the early ontogenesis. Similar to NOD1, RIP2 deficiency significantly affected immune system processes. The significantly enriched pathways were mainly involved in immune system, such as "Antigen processing and presentation" and "NOD-like receptor signaling pathway" and so on. Furthermore, both transcriptome analysis and qRT-PCR revealed that RIP2 was a critical regulator for expression of NLRs (NOD-like receptors) and those genes involved in MHC antigen presentation. Different from NOD1, the present study showed that NOD1, but not RIP2 deficiency significantly impaired protein levels of MAPK pathways. Although RIP2 deficiency also significantly impaired the expression of CD44a, the downstream signaling of CD44a-Lck-PI3K-Akt pathway remained unchanged. Collectively, our works highlight the similarity and discrepancy of NOD1 and RIP2 in the regulation of immune signaling pathways in the zebrafish early ontogenesis, and confirm the crucial role of RIP2 in NLRs signaling and MHC antigen presentation, but not for MAPK and PI3K/Akt pathways.
KeywordRIP2 deficiency larval survival transcriptome analysis signaling pathways NLRs signaling MHC antigen presentation
DOI10.3389/fimmu.2018.00726
Funding OrganizationChinese Academy of Sciences Grant ; Chinese Academy of Sciences Grant ; Chinese Academy of Sciences Grant ; Chinese Academy of Sciences Grant ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences Grant ; Chinese Academy of Sciences Grant ; Chinese Academy of Sciences Grant ; Chinese Academy of Sciences Grant ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences Grant ; Chinese Academy of Sciences Grant ; Chinese Academy of Sciences Grant ; Chinese Academy of Sciences Grant ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences Grant ; Chinese Academy of Sciences Grant ; Chinese Academy of Sciences Grant ; Chinese Academy of Sciences Grant ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
Indexed BySCI ; SCI
Language英语
Funding ProjectChinese Academy of Sciences Grant[XDA08010207] ; National Natural Science Foundation of China[31672687] ; National Natural Science Foundation of China[31372531]
WOS Research AreaImmunology
WOS SubjectImmunology
WOS IDWOS:000429582600001
WOS KeywordNF-KAPPA-B ; INNATE IMMUNE-RESPONSES ; KINASE-ACTIVITY ; NEGATIVE REGULATOR ; TYROSINE KINASES ; VIRUS-INFECTION ; FAMILY-MEMBER ; CELL-DEATH ; RECEPTOR ; ACTIVATION
PublisherFRONTIERS MEDIA SA
Funding OrganizationChinese Academy of Sciences Grant ; Chinese Academy of Sciences Grant ; Chinese Academy of Sciences Grant ; Chinese Academy of Sciences Grant ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences Grant ; Chinese Academy of Sciences Grant ; Chinese Academy of Sciences Grant ; Chinese Academy of Sciences Grant ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences Grant ; Chinese Academy of Sciences Grant ; Chinese Academy of Sciences Grant ; Chinese Academy of Sciences Grant ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences Grant ; Chinese Academy of Sciences Grant ; Chinese Academy of Sciences Grant ; Chinese Academy of Sciences Grant ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
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Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/29676
Collection其他_期刊论文
Corresponding AuthorChang, Ming Xian
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Hubei Vocat Coll Biotechnol, Wuhan, Hubei, Peoples R China
4.Minist Agr, Key Lab Aquaculture Dis Control, Wuhan, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Wu, Xiao Man,Chen, Wen Qin,Hu, Yi Wei,et al. RIP2 Is a Critical Regulator for NLRs Signaling and MHC Antigen Presentation but Not for MAPK and PI3K/Akt Pathways[J]. FRONTIERS IN IMMUNOLOGY,2018,9:18.
APA Wu, Xiao Man,Chen, Wen Qin,Hu, Yi Wei,Cao, Lu,Nie, Pin,&Chang, Ming Xian.(2018).RIP2 Is a Critical Regulator for NLRs Signaling and MHC Antigen Presentation but Not for MAPK and PI3K/Akt Pathways.FRONTIERS IN IMMUNOLOGY,9,18.
MLA Wu, Xiao Man,et al."RIP2 Is a Critical Regulator for NLRs Signaling and MHC Antigen Presentation but Not for MAPK and PI3K/Akt Pathways".FRONTIERS IN IMMUNOLOGY 9(2018):18.
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