| Testing the utility of DNA barcodes and a preliminary phylogenetic framework for Chinese freshwater mussels (Bivalvia: Unionidae) from the middle and lower Yangtze River | |
| Wu, Rui-Wen1; Liu, Yi-Tong1; Wang, Sa1; Liu, Xiong-Jun2; Zanatta, David T.3; Roe, Kevin J.4; Song, Xue-Lin1,5; An, Chang-Ting1,5; Wu, Xiao-Ping1,6 | |
| Corresponding Author | Wu, Xiao-Ping(xpwu@ncu.edu.cn) |
| 2018-08-08 | |
| Source Publication | PLOS ONE
 |
| ISSN | 1932-6203 |
| Volume | 13Issue:8Pages:19 |
| Abstract | The middle and lower portions of the Yangtze River basin is the most species-rich region for freshwater mussels in Asia. The management and conservation of the taxa in this region has been greatly hampered by the lack of a well-developed phylogeny and species-level taxonomic framework. In this study, we tested the utility of two mitochondrial genes commonly used as DNA barcodes: the first subunit of the cytochrome oxidase c gene (COI) and the first subunit of the NADH dehydrogenase gene (ND1) for 34 putative species representing 15 genera, and also generated phylogenetic hypotheses for Chinese unionids based on the combined dataset of the two mitochondrial genes. The results showed that both loci performed well as barcodes for species identification, but the ND1 sequences provided better resolution when compared to COI. Based on the two-locus dataset, Bayesian Inference (BI) and Maximum Likelihood (ML) phylogenetic analyses indicated 3 of the 15 genera of Chinese freshwater mussels examined were polyphyletic. Additionally, the analyses placed the 15 genera into 3 subfamilies: Unioninae (Aculamprotula, Cuneopsis, Nodularia and Schistodesmus), Gonideninae (Lamprotula, Solenaia and Ptychorhychus) and Anodontinae (Cristaria, Arconaia, Acuticosta, Lanceolaria, Anemina and Sinoanodonta). Our results contradict previous taxonomic classification that placed the genera Arconaia, Acuticosta and Lanceolaria in the Unioninae. This study represents one of the first attempts to develop a molecular phylogenetic framework for the Chinese members of the Unionidae and will provide a basis for future research on the evolution, ecology, and conservation of Chinese freshwater mussels. |
| DOI | 10.1371/journal.pone.0200956 |
| Funding Organization | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China |
| Indexed By | SCI ; SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[31772412] |
| WOS Research Area | Science & Technology - Other Topics |
| WOS Subject | Multidisciplinary Sciences |
| WOS ID | WOS:000441090300025 |
| WOS Keyword | MATERNAL MITOCHONDRIAL GENOME ; IDENTIFICATION KEY ; GLOBAL DIVERSITY ; SPECIES CONCEPT ; NORTH-AMERICA ; MOLLUSCA ; SEQUENCES ; GENETICS ; MIDDENDORFF ; SYSTEMATICS |
| Publisher | PUBLIC LIBRARY SCIENCE |
| Funding Organization | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/29490 |
| Collection | 其他_期刊论文 |
| Corresponding Author | Wu, Xiao-Ping |
| Affiliation | 1.Nanchang Univ, Sch Life Sci, Nanchang, Jiangxi, Peoples R China 2.Nanchang Univ, Sch Resources Environm & Chem Engn, Nanchang, Jiangxi, Peoples R China 3.Cent Michigan Univ, Dept Biol, Mt Pleasant, MI 48859 USA 4.Iowa State Univ, Dept Nat Resource Ecol & Management, Ames, IA USA 5.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Hubei, Peoples R China 6.Nanchang Univ, Inst Life Sci, Ctr Watershed Ecol, Nanchang, Jiangxi, Peoples R China |
| Recommended Citation GB/T 7714 | Wu, Rui-Wen,Liu, Yi-Tong,Wang, Sa,et al. Testing the utility of DNA barcodes and a preliminary phylogenetic framework for Chinese freshwater mussels (Bivalvia: Unionidae) from the middle and lower Yangtze River[J]. PLOS ONE,2018,13(8):19. |
| APA | Wu, Rui-Wen.,Liu, Yi-Tong.,Wang, Sa.,Liu, Xiong-Jun.,Zanatta, David T..,...&Wu, Xiao-Ping.(2018).Testing the utility of DNA barcodes and a preliminary phylogenetic framework for Chinese freshwater mussels (Bivalvia: Unionidae) from the middle and lower Yangtze River.PLOS ONE,13(8),19. |
| MLA | Wu, Rui-Wen,et al."Testing the utility of DNA barcodes and a preliminary phylogenetic framework for Chinese freshwater mussels (Bivalvia: Unionidae) from the middle and lower Yangtze River".PLOS ONE 13.8(2018):19. |
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