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Assessment of different mcy genes for detecting the toxic to non-toxic Microcystis ratio in the field by multiplex qPCR
Zuo Jun1,2; Chen Liting1,2; Shan Kun3; Hu Lili1; Song Lirong1; Gan Nanqin1
Corresponding AuthorSong Lirong(lrsong@ihb.ac.cn) ; Gan Nanqin(gannq@ihb.ac.cn)
2018-07-01
Source PublicationJOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN2096-5508
Volume36Issue:4Pages:1132-1144
AbstractHarmful cyanobacterial blooms, especially Microcystis blooms, occur worldwide and draw widespread attention. The dynamics of microcystin-producing Microcystis and competition between microcystin-producing Microcystis and non-microcystin-producing Microcystis are key to predicting and treating Microcystis blooms. Multiplex qPCR is a useful tool to assess such issues. In this study, we developed multiplex qPCR methods with newly-designed probes and primers for the microcystin-synthesis related genes mcyA and mcyE. We used seven toxic Microcystis strains and four non-toxic Microcystis strains to compare the differences in the ratios of toxic and non-toxic Microcystis in mixed cultures, which were calculated using abundances of the genes mcyA, mcyB, mcyD, mcyE and phycocyanin (PC). We also compared traditional cell counting and multiplex qPCR. Hierarchical clustering and principal component analysis indicated that mcyD was the most suitable mcy gene for quantification in laboratory experiments. mcyB abundances were always higher; we suggest that the amount of toxic Microcystis measured using mcyB might overestimate the actual percentages.
KeywordMicrocystis bloom toxic Microcystis multiplex qPCR mcyA mcyB mcyD mcyE PCA
DOI10.1007/s00343-019-7186-1
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Jiangxi Water Science and Technology Fund ; Jiangxi Water Science and Technology Fund ; Jiangxi Water Science and Technology Fund ; Jiangxi Water Science and Technology Fund ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Jiangxi Water Science and Technology Fund ; Jiangxi Water Science and Technology Fund ; Jiangxi Water Science and Technology Fund ; Jiangxi Water Science and Technology Fund ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Jiangxi Water Science and Technology Fund ; Jiangxi Water Science and Technology Fund ; Jiangxi Water Science and Technology Fund ; Jiangxi Water Science and Technology Fund ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Jiangxi Water Science and Technology Fund ; Jiangxi Water Science and Technology Fund ; Jiangxi Water Science and Technology Fund ; Jiangxi Water Science and Technology Fund ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology
Indexed BySCI ; SCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31370418] ; National Natural Science Foundation of China[41561144008] ; Jiangxi Water Science and Technology Fund[KT201602] ; State Key Laboratory of Freshwater Ecology and Biotechnology[2016FBZ07]
WOS Research AreaMarine & Freshwater Biology ; Oceanography
WOS SubjectLimnology ; Oceanography
WOS IDWOS:000441231100011
WOS KeywordREAL-TIME PCR ; CYCLIC HEPTAPEPTIDE MICROCYSTIN ; CYANOBACTERIUM MICROCYSTIS ; FRESH-WATER ; HARMFUL CYANOBACTERIUM ; LAKE DIANCHI ; AERUGINOSA ; SYNTHETASE ; BLOOMS ; DYNAMICS
PublisherSCIENCE PRESS
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Jiangxi Water Science and Technology Fund ; Jiangxi Water Science and Technology Fund ; Jiangxi Water Science and Technology Fund ; Jiangxi Water Science and Technology Fund ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Jiangxi Water Science and Technology Fund ; Jiangxi Water Science and Technology Fund ; Jiangxi Water Science and Technology Fund ; Jiangxi Water Science and Technology Fund ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Jiangxi Water Science and Technology Fund ; Jiangxi Water Science and Technology Fund ; Jiangxi Water Science and Technology Fund ; Jiangxi Water Science and Technology Fund ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Jiangxi Water Science and Technology Fund ; Jiangxi Water Science and Technology Fund ; Jiangxi Water Science and Technology Fund ; Jiangxi Water Science and Technology Fund ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/29480
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorSong Lirong; Gan Nanqin
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Inst Elect Informat Technol, Chongqing 400714, Peoples R China
Recommended Citation
GB/T 7714
Zuo Jun,Chen Liting,Shan Kun,et al. Assessment of different mcy genes for detecting the toxic to non-toxic Microcystis ratio in the field by multiplex qPCR[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2018,36(4):1132-1144.
APA Zuo Jun,Chen Liting,Shan Kun,Hu Lili,Song Lirong,&Gan Nanqin.(2018).Assessment of different mcy genes for detecting the toxic to non-toxic Microcystis ratio in the field by multiplex qPCR.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,36(4),1132-1144.
MLA Zuo Jun,et al."Assessment of different mcy genes for detecting the toxic to non-toxic Microcystis ratio in the field by multiplex qPCR".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 36.4(2018):1132-1144.
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