| Identification and analysis of differentially expressed microRNAs in rainbow trout (Oncorhynchus mykiss) responding to infectious hematopoietic necrosis virus infection | |
| Cao, Yongsheng1,2; Wang, Di1; Li, Shaowu1; Xu, Liming1; Zhao, Jingzhuang1; Liu, Hongbai1; Lu, Tongyan1; Zhang, Qiya2 | |
| Corresponding Author | Lu, Tongyan(lutongyan@hotmail.com) ; Zhang, Qiya(zhangqy@ihb.ac.cn) |
| 2018-11-01 | |
| Source Publication | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| ISSN | 0145-305X |
| Volume | 88Pages:28-36 |
| Abstract | MicroRNAs (miRNAs) are a class of regulators essential for numerous biological processes. Infectious hematopoietic necrosis virus (IHNV) is one of the most important viral pathogens in salmon and trout. In this study, the miRNA expression profiles of rainbow trout upon IHNV infection were explored. In total, 392 known miRNAs and 936 novel miRNAs were identified. Twelve known and 13 novel miRNAs were differentially expressed between infected and uninfected fish. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses revealed that certain miRNA target genes were associated with biological regulation, the immune system, and signal transduction. In addition, over- and suppressed expression of miR-146a-3p, miR-155-5p, miR-216a-5p, and miR-499b-5p could respectively increase and decrease viral gene expression in cells and viral titers. MiR-146a-3p and miR-216a-5p inhibited the expression of type-I IFN and the Mxl gene induced by IHNV. These results provide preliminary insights into the IHNV-host interactions mediated by miRNAs. |
| Keyword | microRNA Rainbow trout IHNV Deep sequencing Functional analysis |
| DOI | 10.1016/j.dci.2018.07.004 |
| Funding Organization | Central-level Nonprofit Scientific Research Institutes Special Funds ; Central-level Nonprofit Scientific Research Institutes Special Funds ; Central-level Nonprofit Scientific Research Institutes Special Funds ; Central-level Nonprofit Scientific Research Institutes Special Funds ; Natural Science Foundation of Heilongjiang Province of China ; Natural Science Foundation of Heilongjiang Province of China ; Natural Science Foundation of Heilongjiang Province of China ; Natural Science Foundation of Heilongjiang Province of China ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Central Public-interest Scientific Institution Basal Research Fund, CAFS ; Central Public-interest Scientific Institution Basal Research Fund, CAFS ; Central Public-interest Scientific Institution Basal Research Fund, CAFS ; Central Public-interest Scientific Institution Basal Research Fund, CAFS ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Central-level Nonprofit Scientific Research Institutes Special Funds ; Central-level Nonprofit Scientific Research Institutes Special Funds ; Central-level Nonprofit Scientific Research Institutes Special Funds ; Central-level Nonprofit Scientific Research Institutes Special Funds ; Natural Science Foundation of Heilongjiang Province of China ; Natural Science Foundation of Heilongjiang Province of China ; Natural Science Foundation of Heilongjiang Province of China ; Natural Science Foundation of Heilongjiang Province of China ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Central Public-interest Scientific Institution Basal Research Fund, CAFS ; Central Public-interest Scientific Institution Basal Research Fund, CAFS ; Central Public-interest Scientific Institution Basal Research Fund, CAFS ; Central Public-interest Scientific Institution Basal Research Fund, CAFS ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Central-level Nonprofit Scientific Research Institutes Special Funds ; Central-level Nonprofit Scientific Research Institutes Special Funds ; Central-level Nonprofit Scientific Research Institutes Special Funds ; Central-level Nonprofit Scientific Research Institutes Special Funds ; Natural Science Foundation of Heilongjiang Province of China ; Natural Science Foundation of Heilongjiang Province of China ; Natural Science Foundation of Heilongjiang Province of China ; Natural Science Foundation of Heilongjiang Province of China ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Central Public-interest Scientific Institution Basal Research Fund, CAFS ; Central Public-interest Scientific Institution Basal Research Fund, CAFS ; Central Public-interest Scientific Institution Basal Research Fund, CAFS ; Central Public-interest Scientific Institution Basal Research Fund, CAFS ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Central-level Nonprofit Scientific Research Institutes Special Funds ; Central-level Nonprofit Scientific Research Institutes Special Funds ; Central-level Nonprofit Scientific Research Institutes Special Funds ; Central-level Nonprofit Scientific Research Institutes Special Funds ; Natural Science Foundation of Heilongjiang Province of China ; Natural Science Foundation of Heilongjiang Province of China ; Natural Science Foundation of Heilongjiang Province of China ; Natural Science Foundation of Heilongjiang Province of China ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Central Public-interest Scientific Institution Basal Research Fund, CAFS ; Central Public-interest Scientific Institution Basal Research Fund, CAFS ; Central Public-interest Scientific Institution Basal Research Fund, CAFS ; Central Public-interest Scientific Institution Basal Research Fund, CAFS ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System |
| Indexed By | SCI ; SCI |
| Language | 英语 |
| Funding Project | Central-level Nonprofit Scientific Research Institutes Special Funds[HSY201702M] ; Natural Science Foundation of Heilongjiang Province of China[C2018073] ; State Key Laboratory of Freshwater Ecology and Biotechnology[2017FB03] ; China Postdoctoral Science Foundation[2016M602395] ; Central Public-interest Scientific Institution Basal Research Fund, CAFS[2017HY-ZD1009] ; Earmarked Fund for China Agriculture Research System[CARS-46] |
| WOS Research Area | Fisheries ; Immunology ; Zoology |
| WOS Subject | Fisheries ; Immunology ; Zoology |
| WOS ID | WOS:000443668600004 |
| WOS Keyword | GROUPER EPINEPHELUS-COIOIDES ; SIGNALING PATHWAY ; VHSV INFECTION ; MIIUY CROAKER ; TELEOST FISH ; APOPTOSIS ; PROFILE ; CELLS ; RNA ; PROLIFERATION |
| Publisher | ELSEVIER SCI LTD |
| Funding Organization | Central-level Nonprofit Scientific Research Institutes Special Funds ; Central-level Nonprofit Scientific Research Institutes Special Funds ; Central-level Nonprofit Scientific Research Institutes Special Funds ; Central-level Nonprofit Scientific Research Institutes Special Funds ; Natural Science Foundation of Heilongjiang Province of China ; Natural Science Foundation of Heilongjiang Province of China ; Natural Science Foundation of Heilongjiang Province of China ; Natural Science Foundation of Heilongjiang Province of China ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Central Public-interest Scientific Institution Basal Research Fund, CAFS ; Central Public-interest Scientific Institution Basal Research Fund, CAFS ; Central Public-interest Scientific Institution Basal Research Fund, CAFS ; Central Public-interest Scientific Institution Basal Research Fund, CAFS ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Central-level Nonprofit Scientific Research Institutes Special Funds ; Central-level Nonprofit Scientific Research Institutes Special Funds ; Central-level Nonprofit Scientific Research Institutes Special Funds ; Central-level Nonprofit Scientific Research Institutes Special Funds ; Natural Science Foundation of Heilongjiang Province of China ; Natural Science Foundation of Heilongjiang Province of China ; Natural Science Foundation of Heilongjiang Province of China ; Natural Science Foundation of Heilongjiang Province of China ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Central Public-interest Scientific Institution Basal Research Fund, CAFS ; Central Public-interest Scientific Institution Basal Research Fund, CAFS ; Central Public-interest Scientific Institution Basal Research Fund, CAFS ; Central Public-interest Scientific Institution Basal Research Fund, CAFS ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Central-level Nonprofit Scientific Research Institutes Special Funds ; Central-level Nonprofit Scientific Research Institutes Special Funds ; Central-level Nonprofit Scientific Research Institutes Special Funds ; Central-level Nonprofit Scientific Research Institutes Special Funds ; Natural Science Foundation of Heilongjiang Province of China ; Natural Science Foundation of Heilongjiang Province of China ; Natural Science Foundation of Heilongjiang Province of China ; Natural Science Foundation of Heilongjiang Province of China ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Central Public-interest Scientific Institution Basal Research Fund, CAFS ; Central Public-interest Scientific Institution Basal Research Fund, CAFS ; Central Public-interest Scientific Institution Basal Research Fund, CAFS ; Central Public-interest Scientific Institution Basal Research Fund, CAFS ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Central-level Nonprofit Scientific Research Institutes Special Funds ; Central-level Nonprofit Scientific Research Institutes Special Funds ; Central-level Nonprofit Scientific Research Institutes Special Funds ; Central-level Nonprofit Scientific Research Institutes Special Funds ; Natural Science Foundation of Heilongjiang Province of China ; Natural Science Foundation of Heilongjiang Province of China ; Natural Science Foundation of Heilongjiang Province of China ; Natural Science Foundation of Heilongjiang Province of China ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; China Postdoctoral Science Foundation ; Central Public-interest Scientific Institution Basal Research Fund, CAFS ; Central Public-interest Scientific Institution Basal Research Fund, CAFS ; Central Public-interest Scientific Institution Basal Research Fund, CAFS ; Central Public-interest Scientific Institution Basal Research Fund, CAFS ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/29411 |
| Collection | 鱼类生物学及渔业生物技术研究中心_期刊论文 |
| Corresponding Author | Lu, Tongyan; Zhang, Qiya |
| Affiliation | 1.Chinese Acad Fishery Sci, Heilongjiang River Fisheries Res Inst, Dept Aquaculture, Lab Fish Dis, Songfa St 43, Harbin 150070, Heilongjiang, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China |
| Recommended Citation GB/T 7714 | Cao, Yongsheng,Wang, Di,Li, Shaowu,et al. Identification and analysis of differentially expressed microRNAs in rainbow trout (Oncorhynchus mykiss) responding to infectious hematopoietic necrosis virus infection[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2018,88:28-36. |
| APA | Cao, Yongsheng.,Wang, Di.,Li, Shaowu.,Xu, Liming.,Zhao, Jingzhuang.,...&Zhang, Qiya.(2018).Identification and analysis of differentially expressed microRNAs in rainbow trout (Oncorhynchus mykiss) responding to infectious hematopoietic necrosis virus infection.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,88,28-36. |
| MLA | Cao, Yongsheng,et al."Identification and analysis of differentially expressed microRNAs in rainbow trout (Oncorhynchus mykiss) responding to infectious hematopoietic necrosis virus infection".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 88(2018):28-36. |
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