IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Molecular cloning and functional characterisation of NLRX1 in grass carp (Ctenopharyngodon idella)
Chu, Pengfei1,2; He, Libo1; Li, Yangyang1,2; Huang, Rong1; Liao, Lanjie1; Li, Yongming1; Zhu, Zuoyan1; Wang, Yaping1
2018-10-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume81Issue:1Pages:276-283
Abstract

The nucleotide-binding domain and leucine-rich-repeat-containing (NLR) proteins regulate innate immunity. Although the positive regulatory impact of NLRs is clear, their inhibitory roles are not well defined. In the present study, the NLR family gene NLRX1 from grass carp (Ctenopharyngodon idella) was cloned and characterised. NLRX1 was widely expressed in all tissues examined, albeit at varying levels. After exposure to the grass carp reovirus (GCRV), NLRX1 mRNA expression levels were altered in immune organs, and dramatically altered in liver. Subcellular localisation indicated that NLRX1 protein co-localised with the mitochondria in the transfected cells. Additionally, the bimolecular fluorescence complementation (BiFC) system was introduced to detect the interaction between tumour necrosis factor (TNF) receptor associated factor 6 (TRAF6) and NLRX1. Moreover, deficient of NLRX1 in CIK cells with small interference RNA (siRNA) promoted polyinosinic:polycytidylic acid (poly (I:C))-induced IFN-related genes production, including IRF3, IRF7, and IFN-I, which reveals that NLRX1 is a negative regulator of IFN. Taken together, our results demonstrate that NLRX1 gene plays an important role in innate immune regulation and provide new insights into understanding the functional characteristics of the NLRX1 in teleosts.

KeywordNLR family Grass carp NLRX1 Innate immune Subcellular localisation Bimolecular fluorescence complementation (BiFC)
DOI10.1016/j.fsi.2018.07.031
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31721005] ; National Natural Science Foundation of China[31702332] ; Hubei Natural Science Foundation of China[2015CFA007] ; Hubei Natural Science Foundation of China[2017CFB374] ; Chinese Academy of Science[XDA08030203]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000443665900032
WOS KeywordNF-KAPPA-B ; INFLAMMATORY RESPONSES ; ANTIVIRAL IMMUNITY ; ADAPTER TRAF6 ; ACTIVATION ; INFECTION ; RECEPTOR ; PATHWAYS ; DISEASE ; PROTEIN
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science ; Chinese Academy of Science
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/29408
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorWang, Yaping
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Chu, Pengfei,He, Libo,Li, Yangyang,et al. Molecular cloning and functional characterisation of NLRX1 in grass carp (Ctenopharyngodon idella)[J]. FISH & SHELLFISH IMMUNOLOGY,2018,81(1):276-283.
APA Chu, Pengfei.,He, Libo.,Li, Yangyang.,Huang, Rong.,Liao, Lanjie.,...&Wang, Yaping.(2018).Molecular cloning and functional characterisation of NLRX1 in grass carp (Ctenopharyngodon idella).FISH & SHELLFISH IMMUNOLOGY,81(1),276-283.
MLA Chu, Pengfei,et al."Molecular cloning and functional characterisation of NLRX1 in grass carp (Ctenopharyngodon idella)".FISH & SHELLFISH IMMUNOLOGY 81.1(2018):276-283.
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