IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Transcript-associated microsatellites from gibel carpand their applicability of genetic analyses in Carassius auratus populations
Wang, Xinhua1,2; Gan, Baojiang1; Yu, Xiaomu1; Zhou, Li1; Wang, Zhongwei1; Gui, Jianfang1; Yin, Zhan1; Tong, Jingou1
Corresponding AuthorTong, Jingou(jgtong@ihb.ac.cn)
2018-10-01
Source PublicationJOURNAL OF APPLIED ICHTHYOLOGY
ISSN0175-8659
Volume34Issue:5Pages:1108-1116
AbstractMicrosatellites are popular co-dominant markers for different genetic analyses, and transcriptome sequencing provides a new approach for rapid development of microsatellite markers. Gibel carp is a triploid gynogenetic fish with great growth potentials, but no transcript-associated microsatellite markers are available. Here we developed and characterized a set of EST-SSRs from the transcriptome data of gibel carp. 126 out of the 150 EST-SSRs were successfully developed and 110 showed polymorphisms in gibel carp. Twenty-four randomly selected polymorphic EST-SSRs were characterized in a cultured population of gibel carp, and the number of alleles per locus ranged from 3 to 9 (mean 6.5), with an average proportion of heterozygotes of 84.2%. All 24 EST-SSRs were successfully amplified in a goldfish family, and genotypes of all loci were in the Mendelian expectations after Bonferroni corrections. In addition, seven polymorphic EST-SSRs were selected for genetic diversity analysis in seven wild crucian carp populations of China, and the results showed that these loci have abundant polymorphisms with allele numbers of 13-37. Taken together with the genetic variations of crucian carp populations, the average number of alleles (N-a) was 20.9 per locus, and the mean H-o, H-e and PIC were 0.752, 0.878 and 0.865, respectively. Structure analysis and clustering results consistently supported that seven crucian carp populations could be divided into two major branches, and the genetic distances are basically positively correlated with geographical distances. These transcript-derived microsatellites are valuable resources and powerful tools for population genetics and many other genetic analyses in gibel carp and its close relatives.
Keywordcross-strain amplification Gibel carp microsatellites polymorphism population genetic analyses
DOI10.1111/jai.13762
Funding OrganizationSpecial Fund for Strategic Pilot Technology of CAS ; Special Fund for Strategic Pilot Technology of CAS ; Special Fund for Strategic Pilot Technology of CAS ; Special Fund for Strategic Pilot Technology of CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology of China ; State Key Laboratory of Freshwater Ecology and Biotechnology of China ; State Key Laboratory of Freshwater Ecology and Biotechnology of China ; State Key Laboratory of Freshwater Ecology and Biotechnology of China ; Special Fund for Strategic Pilot Technology of CAS ; Special Fund for Strategic Pilot Technology of CAS ; Special Fund for Strategic Pilot Technology of CAS ; Special Fund for Strategic Pilot Technology of CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology of China ; State Key Laboratory of Freshwater Ecology and Biotechnology of China ; State Key Laboratory of Freshwater Ecology and Biotechnology of China ; State Key Laboratory of Freshwater Ecology and Biotechnology of China ; Special Fund for Strategic Pilot Technology of CAS ; Special Fund for Strategic Pilot Technology of CAS ; Special Fund for Strategic Pilot Technology of CAS ; Special Fund for Strategic Pilot Technology of CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology of China ; State Key Laboratory of Freshwater Ecology and Biotechnology of China ; State Key Laboratory of Freshwater Ecology and Biotechnology of China ; State Key Laboratory of Freshwater Ecology and Biotechnology of China ; Special Fund for Strategic Pilot Technology of CAS ; Special Fund for Strategic Pilot Technology of CAS ; Special Fund for Strategic Pilot Technology of CAS ; Special Fund for Strategic Pilot Technology of CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology of China ; State Key Laboratory of Freshwater Ecology and Biotechnology of China ; State Key Laboratory of Freshwater Ecology and Biotechnology of China ; State Key Laboratory of Freshwater Ecology and Biotechnology of China
Indexed BySCI ; SCI
Language英语
Funding ProjectSpecial Fund for Strategic Pilot Technology of CAS[XDA08010405] ; State Key Laboratory of Freshwater Ecology and Biotechnology of China[2016FBZ05]
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000444542800002
WOS KeywordSILVER CRUCIAN CARP ; EST-SSR MARKERS ; MULTILOCUS GENOTYPE DATA ; CROSS-AMPLIFICATION ; COMMON CARP ; BLOCH ; LOCI ; DIVERSITY ; INFERENCE ; FISH
PublisherWILEY
Funding OrganizationSpecial Fund for Strategic Pilot Technology of CAS ; Special Fund for Strategic Pilot Technology of CAS ; Special Fund for Strategic Pilot Technology of CAS ; Special Fund for Strategic Pilot Technology of CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology of China ; State Key Laboratory of Freshwater Ecology and Biotechnology of China ; State Key Laboratory of Freshwater Ecology and Biotechnology of China ; State Key Laboratory of Freshwater Ecology and Biotechnology of China ; Special Fund for Strategic Pilot Technology of CAS ; Special Fund for Strategic Pilot Technology of CAS ; Special Fund for Strategic Pilot Technology of CAS ; Special Fund for Strategic Pilot Technology of CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology of China ; State Key Laboratory of Freshwater Ecology and Biotechnology of China ; State Key Laboratory of Freshwater Ecology and Biotechnology of China ; State Key Laboratory of Freshwater Ecology and Biotechnology of China ; Special Fund for Strategic Pilot Technology of CAS ; Special Fund for Strategic Pilot Technology of CAS ; Special Fund for Strategic Pilot Technology of CAS ; Special Fund for Strategic Pilot Technology of CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology of China ; State Key Laboratory of Freshwater Ecology and Biotechnology of China ; State Key Laboratory of Freshwater Ecology and Biotechnology of China ; State Key Laboratory of Freshwater Ecology and Biotechnology of China ; Special Fund for Strategic Pilot Technology of CAS ; Special Fund for Strategic Pilot Technology of CAS ; Special Fund for Strategic Pilot Technology of CAS ; Special Fund for Strategic Pilot Technology of CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology of China ; State Key Laboratory of Freshwater Ecology and Biotechnology of China ; State Key Laboratory of Freshwater Ecology and Biotechnology of China ; State Key Laboratory of Freshwater Ecology and Biotechnology of China
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/29374
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorTong, Jingou
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China
2.Henan Univ Anim Husb & Econ, Coll Anim Sci & Technol, Zhengzhou, Henan, Peoples R China
Recommended Citation
GB/T 7714
Wang, Xinhua,Gan, Baojiang,Yu, Xiaomu,et al. Transcript-associated microsatellites from gibel carpand their applicability of genetic analyses in Carassius auratus populations[J]. JOURNAL OF APPLIED ICHTHYOLOGY,2018,34(5):1108-1116.
APA Wang, Xinhua.,Gan, Baojiang.,Yu, Xiaomu.,Zhou, Li.,Wang, Zhongwei.,...&Tong, Jingou.(2018).Transcript-associated microsatellites from gibel carpand their applicability of genetic analyses in Carassius auratus populations.JOURNAL OF APPLIED ICHTHYOLOGY,34(5),1108-1116.
MLA Wang, Xinhua,et al."Transcript-associated microsatellites from gibel carpand their applicability of genetic analyses in Carassius auratus populations".JOURNAL OF APPLIED ICHTHYOLOGY 34.5(2018):1108-1116.
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