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题名: GABAergic Neurons and Their Modulatory Effects on GnRH3 in Zebrafish
作者: Song, Yanlong1; Tao, Binbin1; Chen, Ji1; Jia, Shaoting1; Zhu, Zuoyan1; Trudeau, Vance L.2; Hu, Wei1
刊名: ENDOCRINOLOGY
发表日期: 2017-04-01
DOI: 10.1210/en.2016-1776
卷: 158, 期:4, 页:874-886
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Endocrinology & Metabolism
研究领域[WOS]: Endocrinology & Metabolism
英文摘要: gamma-Aminobutyric acid (GABA) is a major amino acid neurotransmitter in the vertebrate brain. To provide detailed information on the distribution of the GABA in zebrafish (Danio rerio), neurons were labeled with mCherry driven by the glutamic acid decarboxylase 67 (gad67) promoter. In the transgenic line Tg(gad67: mCherry), mCherry-positive gad67 cell bodies were predominantly localized to the olfactory bulb, pallial zones, subpallium zones, parvocellular preoptic nucleus, periventricular gray zone of optic tectum, torus semicircularis, posterior tuberculum, medial longitudinal fascicle, caudal zone of periventricular hypothalamus, and oculomotor nucleus. mCherry-positive fibers were widely distributed in the olfactory bulbs, subpallium, thalamus, ventral hypothalamic zone, tectumopticum, mesencephalon, and rhombencephalon. mCherry-positive neurons were also observed in the retina and the spinal cord. The anatomical relationships between GABAergic and gonadotrophin-releasing hormone 3 (GnRH3) neurons were investigated by crossing Tg(gad67: mCherry) fish with the previously established Tg(gnrh3: EGFP) transgenic line. GnRH3 cell bodies and fibers were contacted by GABAergic fibers directly in the ventral telencephalon and anterior tuberal nucleus. A subpopulation of GnRH3 neurons in the ventral telencephalic area was also labeled with mCherry, so some GnRH3 neurons are also GABAergic. GABA(B) receptor agonist (baclofen) and antagonist (CGP55845) treatments indicated that GABA(B) receptor signaling inhibited gnrh3 expression in larval fish but was stimulatory in adult fish. The expression of pituitary lh beta and fsh beta was stimulated by intraperitoneal injection of baclofen in adult fish. We conclude that GABA via GABAB receptors regulates GnRH3 neurons in a developmentally dependent manner in zebrafish.
关键词[WOS]: GONADOTROPIN-RELEASING-HORMONE ; GAMMA-AMINOBUTYRIC-ACID ; MESSENGER-RNA EXPRESSION ; GOLDFISH CARASSIUS-AURATUS ; IMMUNOCYTOCHEMICAL LOCALIZATION ; DECARBOXYLASE GENES ; PETROMYZON-MARINUS ; RAINBOW-TROUT ; LHRH NEURONS ; SEA LAMPREY
语种: 英语
项目资助者: National Natural Science Foundation(31325026) ; Chinese Academy of Sciences(XDA08010106 ; Natural Sciences and Engineering Research Council of Canada ; University of Ottawa International Research Acceleration Program ; 2016FBZ03)
WOS记录号: WOS:000398718200019
ISSN号: 0013-7227
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/29211
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Univ Ottawa, Dept Biol, Ottawa, ON K1N 6N5, Canada
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