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题名: Characterization of dominant and cellulolytic bacterial communities along the gut of silver carp Hypophthalmichthys molitrix during cyanobacterial blooms
作者: Luo Congqiang1, 2; Yi Chunlong1, 2; Ni Leyi1; Guo Longgen1
关键词: silver carp ; bacteria communities ; PCR-DGGE ; qPCR
刊名: CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
发表日期: 2017-05-01
DOI: 10.1007/s00343-017-5253-z
卷: 35, 期:3, 页:624-633
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]: Limnology ; Oceanography
研究领域[WOS]: Marine & Freshwater Biology ; Oceanography
英文摘要: Silver carp is one of the most important planktivorous fish in Chinese aquaculture and plays a significant role controlling cyanobacterial blooms. A balanced gut microbiota is crucial for growth and health of the host because of its important roles in immune defense, digestion of complex carbohydrates, and production of enterocytes. In our study, the dominant bacterial and cellulolytic bacterial (Clostridium I, Clostridium III, Clostridium XIVab, and Fibrobacter) communities in the contents and mucus of the silver carp gut (foregut, midgut, and hindgut) were analyzed by denaturing gradient gel electrophoresis and quantitative polymerase chain reaction (qPCR) analyses. The results revealed that the dominant and cellulolytic bacterial communities were significantly different among gut regions as well as in contents and mucus. Bacterial diversity and richness in contents and mucus increased along the gut and were higher in contents than those in local mucus. A sequence analysis of gut samples exhibited the conservative phylotypes of Proteobacteria, Actinobacteria, and Firmicutes. The gut of silver carp harbored an abundance of cellulolytic bacteria, particularly Clostridium XIV ab. The foregut segment had the highest proportions of the four cellulolytic bacteria, followed by the midgut and hindgut. However, the proportions of cellulolytic species in the silver carp gut was much lower than those in the terrestrial vertebrate gastrointestinal tract. We conclude that gut bacteria could help silver carp obtain energy from cyanobacteria, which may be why silver carp can maintain high growth rates during cyanobacterial blooms.
关键词[WOS]: TOXIC MICROCYSTIS BLOOMS ; IN-SITU HYBRIDIZATION ; LAKE TAIHU ; MICROBIAL DIVERSITY ; INTESTINAL CONTENTS ; LANDFILL SITES ; POPULATIONS ; FISH ; ABUNDANCE ; PASSAGE
语种: 英语
项目资助者: National Science and Technology Ministry(2014BAC09B02) ; National Water Pollution Control and Management Technology Major Projects(2012ZX07101-002)
WOS记录号: WOS:000399168400017
ISSN号: 0254-4059
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/29205
Appears in Collections:淡水生态学研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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