Malonylome Analysis Reveals the Involvement of Lysine Malonylation in Metabolism and Photosynthesis in Cyanobacteria
Ma, Yanyan1,2; Yang, Mingkun1; Lin, Xiaohuang1,2; Liu, Xin1,2; Huang, Hui1,2; Ge, Feng1
2017-05-01
Source PublicationJOURNAL OF PROTEOME RESEARCH
ISSN1535-3893
Volume16Issue:5Pages:2030-2043
AbstractAs a recently validated reversible post translational modification, lysine malonylation regulates diverse cellular processes from bacteria to mammals, but its existence and function in photosynthetic organisms remain unknown. Cyanobacteria are the most ancient group of photosynthetic prokaryotes and contribute about 50% of the total primary production on Earth. Previously, we reported the lysine acetylome in the model cyanobacterium Synechocystis sp. PCC 6803 (Synechocystis). Here we performed the first proteomic survey of lysine malonylation in Synechocystis using highly accurate tandem mass spectrometry in combination with affinity purification. We identified S98 lysine malonylation sites on 339 proteins with high confidence in total. A bioinformatic analysis suggested that these malonylated proteins may play various functions and were distributed in diverse subcellular compartments. Among them, many malonylated proteins were involved in cellular metabolism. The functional significance of lysine malonylation in the metabolic enzyme activity of phosphoglycerate kinase (PGK) was determined by site-specific mutagenesis and biochemical studies. Interestingly, 27 proteins involved in photosynthesis were found to be malonylated for the first time, suggesting that lysine malonylation may be involved in photosynthesis. Thus our results provide the first lysine malonylome in a photosynthetic organism and suggest a previously unexplored role of lysine malonylation in the regulation of metabolic processes and photosynthesis in Synechocystis as well as in other photosynthetic organisms.
SubtypeArticle
KeywordLysine Malonylation Post-translational Modification (Ptm) Cyanobacteria Synechocystis Photosynthesis Phosphoglycerate Kinase (Pgk) Metabolism
DOI10.1021/acs.jproteome.7b00017
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Key Research and Development Program(2016YFA0501304) ; National Key Research and Development Program(2016YFA0501304) ; Chinese Academy of Sciences Grant(QYZDY-SSW-SMC004) ; Chinese Academy of Sciences Grant(QYZDY-SSW-SMC004) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14030202) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14030202) ; National Key Research and Development Program(2016YFA0501304) ; National Key Research and Development Program(2016YFA0501304) ; Chinese Academy of Sciences Grant(QYZDY-SSW-SMC004) ; Chinese Academy of Sciences Grant(QYZDY-SSW-SMC004) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14030202) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14030202)
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemical Research Methods
WOS KeywordSP PCC 6803 ; ACETYLOME ANALYSIS REVEALS ; PHOSPHOGLYCERATE KINASE ; POSTTRANSLATIONAL MODIFICATIONS ; MYCOBACTERIUM-TUBERCULOSIS ; CHLAMYDOMONAS-REINHARDTII ; SUCCINYLATION PROFILES ; PEPTIDE IDENTIFICATION ; PLASMODIUM-FALCIPARUM ; THERMUS-THERMOPHILUS
WOS IDWOS:000401044600018
Funding OrganizationNational Key Research and Development Program(2016YFA0501304) ; National Key Research and Development Program(2016YFA0501304) ; Chinese Academy of Sciences Grant(QYZDY-SSW-SMC004) ; Chinese Academy of Sciences Grant(QYZDY-SSW-SMC004) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14030202) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14030202) ; National Key Research and Development Program(2016YFA0501304) ; National Key Research and Development Program(2016YFA0501304) ; Chinese Academy of Sciences Grant(QYZDY-SSW-SMC004) ; Chinese Academy of Sciences Grant(QYZDY-SSW-SMC004) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14030202) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14030202)
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Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/29189
Collection藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Ma, Yanyan,Yang, Mingkun,Lin, Xiaohuang,et al. Malonylome Analysis Reveals the Involvement of Lysine Malonylation in Metabolism and Photosynthesis in Cyanobacteria[J]. JOURNAL OF PROTEOME RESEARCH,2017,16(5):2030-2043.
APA Ma, Yanyan,Yang, Mingkun,Lin, Xiaohuang,Liu, Xin,Huang, Hui,&Ge, Feng.(2017).Malonylome Analysis Reveals the Involvement of Lysine Malonylation in Metabolism and Photosynthesis in Cyanobacteria.JOURNAL OF PROTEOME RESEARCH,16(5),2030-2043.
MLA Ma, Yanyan,et al."Malonylome Analysis Reveals the Involvement of Lysine Malonylation in Metabolism and Photosynthesis in Cyanobacteria".JOURNAL OF PROTEOME RESEARCH 16.5(2017):2030-2043.
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