Quantitative analysis of glutathione and cysteine S-conjugates of microcystin-LR in the liver, kidney and muscle of common carp (Cyprinus carpio) in Lake Taihu
Environmental Sciences
; Public, Environmental & Occupational Health
; Microbiology
; Water Resources
研究领域[WOS]:
Environmental Sciences & Ecology
; Public, Environmental & Occupational Health
; Microbiology
; Water Resources
英文摘要:
Tissue distribution of microcystin (MC)-LR-GSH, MC-LR-Cys and MC-LR of omnivorous fish in Lake Taihu was investigated. MC-LR and MC-LR-Cys were detected in liver, kidney and muscle. The concentration of MC-LR in liver and kidney was 0.052 mu g g(-1) DW and 0.067 mu g g(-1) DW, respectively. MC-LR-Cys appeared to be an important metabolite with average contents of 1.104 mu g g(-1) DW and 0.724 mu g g(-1) DW in liver and kidney, and the MC-LR-Cys/MC-LR ratio in liver and kidney reaching as high as 21.4 and 10.8. High MC-LR-Cys/MC-LR ratio and a significant correlation between MC-LR-Cys and MC-LR concentration in liver, suggest that liver is more active in detoxification of MC-LR by formation of MC-LR-Cys for omnivorous fish. Furthermore, there might be a balance between the accumulation and depuration/metabolism of MC-LR-Cys in kidney. The MC-LR-Cys can be formed in kidney directly, or transported from liver or other tissues, while the MC-LR-Cys in kidney might be dissociated to MC-LR or excreted. Although MC-LR and its metabolites were scarcely detected in muscle, it is necessary to investigate the distribution of toxic metabolites in edible muscle.
关键词[WOS]:
TANDEM MASS-SPECTROMETRY
; CYANOBACTERIAL TOXINS
; FISH
; DETOXIFICATION
; DETOXICATION
; METABOLITES
; NOBILIS
; CHINA
; GSH
; RR
语种:
英语
项目资助者:
State Key Laboratory of Freshwater Ecology and Biotechnology(2014FBZ02)
; National Natural Science Foundations of China(21307164
; Study Abroad for CSC Sponsored Chinese Citizen
; Academic Research Abroad from South-Central University for Nationalities Sponsored Outstanding Young Teacher
; 31200361)
