| Molecular characterization of the giant freshwater prawn (Macrobrachium rosenbergii) beta-actin gene promoter | |
Yan, Junjun1,2; Gao, Qiang3; Cui, Zongbin1; Yang, Guoliang4; Long, Yong1
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| Corresponding Author | Yang, Guoliang(ygl0572@163.com) ; Long, Yong(longyong@ihb.ac.cn) |
| 2018-10-23 | |
| Source Publication | PEERJ
 |
| ISSN | 2167-8359 |
| Volume | 6Pages:18 |
| Abstract | Constitutive promoters are important tools for gene function studies and transgenesis. The Beta-actin (actb1) gene promoter has been isolated from many species but remains to be cloned from the giant freshwater prawn (Macrobrachium rosenbergii). In this study, we cloned and characterized the Mractb1 gene promoter. Two alternative promoters were identified for the Mractb1 gene, which direct the generation of two transcripts with different 5' untranslated regions. Three CpG islands were predicted in the upstream sequence, which are intimately related to transcription initiation and promoter activity. In addition to the CCAAT-box and the CArG-box, molecular dissection of the flanking sequence revealed the existence of one negative and two positive elements in the upstream region and the first intron. Finally, the Mractb1 promoter demonstrated comparative activity to the carp (Cyprinus carpio) actb1 promoter. Our investigations provide a valuable genetic tool for gene function studies and shed light on the regulation of the Mractb1 gene. |
| Keyword | Giant freshwater prawn Beta-actin gene Promoter Transcriptional activity |
| DOI | 10.7717/peerj.5701 |
| Funding Organization | Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province |
| Indexed By | SCI ; SCI |
| Language | 英语 |
| Funding Project | Youth Innovation Promotion Association of Chinese Academy of Sciences[2016305] ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province[2012C12907-2] |
| WOS Research Area | Science & Technology - Other Topics |
| WOS Subject | Multidisciplinary Sciences |
| WOS ID | WOS:000448082800004 |
| WOS Keyword | FUNCTIONAL-ANALYSIS ; GENOMIC CLONING ; EXPRESSION ; CARP ; TRANSCRIPTION ; REPORTER ; DISEASES ; EMBRYOS ; TILAPIA ; REGION |
| Publisher | PEERJ INC |
| Funding Organization | Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/29180 |
| Collection | 水生生物分子与细胞生物学研究中心_期刊论文 |
| Corresponding Author | Yang, Guoliang; Long, Yong |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Zhejiang Inst Freshwater Fisheries, Huzhou, Peoples R China 4.Huzhou Univ, Huzhou, Peoples R China |
| Recommended Citation GB/T 7714 | Yan, Junjun,Gao, Qiang,Cui, Zongbin,et al. Molecular characterization of the giant freshwater prawn (Macrobrachium rosenbergii) beta-actin gene promoter[J]. PEERJ,2018,6:18. |
| APA | Yan, Junjun,Gao, Qiang,Cui, Zongbin,Yang, Guoliang,&Long, Yong.(2018).Molecular characterization of the giant freshwater prawn (Macrobrachium rosenbergii) beta-actin gene promoter.PEERJ,6,18. |
| MLA | Yan, Junjun,et al."Molecular characterization of the giant freshwater prawn (Macrobrachium rosenbergii) beta-actin gene promoter".PEERJ 6(2018):18. |
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