IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Molecular characterization of the giant freshwater prawn (Macrobrachium rosenbergii) beta-actin gene promoter
Yan, Junjun1,2; Gao, Qiang3; Cui, Zongbin1; Yang, Guoliang4; Long, Yong1
Corresponding AuthorYang, Guoliang(ygl0572@163.com) ; Long, Yong(longyong@ihb.ac.cn)
2018-10-23
Source PublicationPEERJ
ISSN2167-8359
Volume6Pages:18
AbstractConstitutive promoters are important tools for gene function studies and transgenesis. The Beta-actin (actb1) gene promoter has been isolated from many species but remains to be cloned from the giant freshwater prawn (Macrobrachium rosenbergii). In this study, we cloned and characterized the Mractb1 gene promoter. Two alternative promoters were identified for the Mractb1 gene, which direct the generation of two transcripts with different 5' untranslated regions. Three CpG islands were predicted in the upstream sequence, which are intimately related to transcription initiation and promoter activity. In addition to the CCAAT-box and the CArG-box, molecular dissection of the flanking sequence revealed the existence of one negative and two positive elements in the upstream region and the first intron. Finally, the Mractb1 promoter demonstrated comparative activity to the carp (Cyprinus carpio) actb1 promoter. Our investigations provide a valuable genetic tool for gene function studies and shed light on the regulation of the Mractb1 gene.
KeywordGiant freshwater prawn Beta-actin gene Promoter Transcriptional activity
DOI10.7717/peerj.5701
Funding OrganizationYouth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province
Indexed BySCI ; SCI
Language英语
Funding ProjectYouth Innovation Promotion Association of Chinese Academy of Sciences[2016305] ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province[2012C12907-2]
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000448082800004
WOS KeywordFUNCTIONAL-ANALYSIS ; GENOMIC CLONING ; EXPRESSION ; CARP ; TRANSCRIPTION ; REPORTER ; DISEASES ; EMBRYOS ; TILAPIA ; REGION
PublisherPEERJ INC
Funding OrganizationYouth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Youth Innovation Promotion Association of Chinese Academy of Sciences ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/29180
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorYang, Guoliang; Long, Yong
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Zhejiang Inst Freshwater Fisheries, Huzhou, Peoples R China
4.Huzhou Univ, Huzhou, Peoples R China
Recommended Citation
GB/T 7714
Yan, Junjun,Gao, Qiang,Cui, Zongbin,et al. Molecular characterization of the giant freshwater prawn (Macrobrachium rosenbergii) beta-actin gene promoter[J]. PEERJ,2018,6:18.
APA Yan, Junjun,Gao, Qiang,Cui, Zongbin,Yang, Guoliang,&Long, Yong.(2018).Molecular characterization of the giant freshwater prawn (Macrobrachium rosenbergii) beta-actin gene promoter.PEERJ,6,18.
MLA Yan, Junjun,et al."Molecular characterization of the giant freshwater prawn (Macrobrachium rosenbergii) beta-actin gene promoter".PEERJ 6(2018):18.
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