Divergent Expression Patterns and Function of Two cxcr4 Paralogs in Hermaphroditic Epinephelus coioides

doi:10.3390/ijms19102943

	Divergent Expression Patterns and Function of Two cxcr4 Paralogs in Hermaphroditic Epinephelus coioides
	Lu, Wei-Jia 1,2; Zhou, Li 1,2; Gao, Fan-Xiang 1,2; Sun, Zhi-Hui 3; Li, Zhi1 ; Liu, Xiao-Chun 4,5; Li, Shui-Sheng 4,5; Wang, Yang 1,2; Gui, Jian-Fang 1,2
Corresponding Author	Wang, Yang(wangyang@ihb.ac.cn) ; Gui, Jian-Fang(jfgui@ihb.ac.cn)
	2018-10-01
Source Publication	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN	1422-0067
Volume	19 Issue:10 Pages:19
Abstract	Chemokine receptor Cxcr4 evolved two paralogs in the teleost lineage. However, cxcr4a and cxcr4b have been characterized only in a few species. In this study, we identified two cxcr4 paralogs from the orange-spotted grouper, Epinephelus coioides. The phylogenetic relationship and gene structure and synteny suggest that the duplicated cxcr4a/b should result from the teleost-specific genome duplication (Ts3R). The teleost cxcr4 gene clusters in two paralogous chromosomes exhibit a complementary gene loss/retention pattern. Ec_cxcr4a and Ec_cxcr4b show differential and biased expression patterns in grouper adult tissue, gonads, and embryos at different stages. During embryogenesis, Ec_cxcr4a/b are abundantly transcribed from the neurula stage and mainly expressed in the neural plate and sensory organs, indicating their roles in neurogenesis. Ec_Cxcr4a and Ec_Cxcr4b possess different chemotactic migratory abilities from the human SDF-1, Ec_Cxcl12a, and Ec_Cxcl12b. Moreover, we uncovered the N-terminus and TM5 domain as the key elements for specific ligand-receptor recognition of Ec_Cxcr4a-Ec_Cxcl12b and Ec_Cxcr4b-Ec_Cxcl12a. Based on the biased and divergent expression patterns of Eccxcr4a/b, and specific ligand-receptor recognition of Ec_Cxcl12a/b-Ec_Cxcr4b/a, the current study provides a paradigm of sub-functionalization of two teleost paralogs after Ts3R.
Keyword	cxcr4 paralog biased and divergent expression chemotaxis ability orange-spotted grouper sub-functionalization
DOI	10.3390/ijms19102943
Funding Organization	National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences
Indexed By	SCI ; SCI
Language	英语
Funding Project	National Natural Science Foundation of China[U1401213] ; Chinese Academy of Sciences[QYZDY-SWS-SMC025]
WOS Research Area	Biochemistry & Molecular Biology ; Chemistry
WOS Subject	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS ID	WOS:000448951000098
WOS Keyword	VERTEBRATE GENOME EVOLUTION ; CHEMOKINE RECEPTOR CXCR4 ; CENTRAL-NERVOUS-SYSTEM ; GUIDED CELL-MIGRATION ; MOLECULAR CHARACTERIZATION ; TELEOST FISH ; ALLOPOLYPLOID COTTON ; PROVIDES INSIGHTS ; GENE DUPLICATION ; LEVEL DOMINANCE
Publisher	MDPI
Funding Organization	National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences
Citation statistics	Cited Times:2[WOS] [WOS Record] [Related Records in WOS]
Document Type	期刊论文
Identifier	http://ir.ihb.ac.cn/handle/342005/29152
Collection	鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding Author	Wang, Yang; Gui, Jian-Fang
Affiliation	1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Dalian Ocean Univ, Minist Agr & Rural Affairs, Key Lab Mariculture & Stock Enhancement North Chi, Dalian 116023, Peoples R China 4.Sun Yat Sen Univ, Guangdong Prov Key Lab Aquat Econ Anim, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China 5.Sun Yat Sen Univ, Inst Aquat Econ Anim, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China
Recommended Citation GB/T 7714	Lu, Wei-Jia,Zhou, Li,Gao, Fan-Xiang,et al. Divergent Expression Patterns and Function of Two cxcr4 Paralogs in Hermaphroditic Epinephelus coioides[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2018,19(10):19.
APA	Lu, Wei-Jia.,Zhou, Li.,Gao, Fan-Xiang.,Sun, Zhi-Hui.,Li, Zhi.,...&Gui, Jian-Fang.(2018).Divergent Expression Patterns and Function of Two cxcr4 Paralogs in Hermaphroditic Epinephelus coioides.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,19(10),19.
MLA	Lu, Wei-Jia,et al."Divergent Expression Patterns and Function of Two cxcr4 Paralogs in Hermaphroditic Epinephelus coioides".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 19.10(2018):19.

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