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Crystal-on-crystal chips for in situ serial diffraction at room temperature
Ren, Zhong1,3; Ayhan, Medine1; Bandara, Sepatika1; Bowatte, Katinga1; Kumarapperuma, Indika1; Gunawardana, Semini1; Shin, Heewhan1; Wang, Cong1; Zeng, Xiaoti1,4; Yang, Xiaojing1,2
Corresponding AuthorRen, Zhong(zren@uic.edu) ; Yang, Xiaojing(xiaojing@uic.edu)
2018-08-07
Source PublicationLAB ON A CHIP
ISSN1473-0197
Volume18Issue:15Pages:2246-2256
AbstractRecent developments in serial crystallography at X-ray free electron lasers (XFELs) and synchrotrons have been driven by two scientific goals in structural biology - first, static structure determination from nano or microcrystals of membrane proteins and large complexes that are difficult for conventional cryocrystallography, and second, direct observations of transient structural species in biochemical reactions at near atomic resolution. Since room-temperature diffraction experiments naturally demand a large quantity of purified protein, sample economy is critically important for all steps of serial crystallography from crystallization, crystal delivery to data collection. Here we report the development and applications of "crystal-on-crystal" devices to facilitate large-scale in situ serial diffraction experiments on protein crystals of all sizes - large, small or microscopic. We show that the monocrystalline quartz as a substrate material prevents vapor loss during crystallization and significantly reduces background X-ray scattering. These devices can be readily adopted at XFEL and synchrotron beamlines, which enable efficient delivery of hundreds to millions of crystals to the X-ray beam, with an overall protein consumption per dataset comparable to that of cryocrystallography.
DOI10.1039/c8lc00489g
Funding OrganizationUS Department of Energy ; US Department of Energy ; US Department of Energy ; US Department of Energy ; National Institute of General Medical Sciences of the National Institutes of Health ; National Institute of General Medical Sciences of the National Institutes of Health ; National Institute of General Medical Sciences of the National Institutes of Health ; National Institute of General Medical Sciences of the National Institutes of Health ; Michigan Economic Development Corporation ; Michigan Economic Development Corporation ; Michigan Economic Development Corporation ; Michigan Economic Development Corporation ; Michigan Technology Tri-Corridor ; Michigan Technology Tri-Corridor ; Michigan Technology Tri-Corridor ; Michigan Technology Tri-Corridor ; National Institutes of Health ; National Institutes of Health ; National Institutes of Health ; National Institutes of Health ; University of Illinois at Chicago ; University of Illinois at Chicago ; University of Illinois at Chicago ; University of Illinois at Chicago ; US Department of Energy ; US Department of Energy ; US Department of Energy ; US Department of Energy ; National Institute of General Medical Sciences of the National Institutes of Health ; National Institute of General Medical Sciences of the National Institutes of Health ; National Institute of General Medical Sciences of the National Institutes of Health ; National Institute of General Medical Sciences of the National Institutes of Health ; Michigan Economic Development Corporation ; Michigan Economic Development Corporation ; Michigan Economic Development Corporation ; Michigan Economic Development Corporation ; Michigan Technology Tri-Corridor ; Michigan Technology Tri-Corridor ; Michigan Technology Tri-Corridor ; Michigan Technology Tri-Corridor ; National Institutes of Health ; National Institutes of Health ; National Institutes of Health ; National Institutes of Health ; University of Illinois at Chicago ; University of Illinois at Chicago ; University of Illinois at Chicago ; University of Illinois at Chicago ; US Department of Energy ; US Department of Energy ; US Department of Energy ; US Department of Energy ; National Institute of General Medical Sciences of the National Institutes of Health ; National Institute of General Medical Sciences of the National Institutes of Health ; National Institute of General Medical Sciences of the National Institutes of Health ; National Institute of General Medical Sciences of the National Institutes of Health ; Michigan Economic Development Corporation ; Michigan Economic Development Corporation ; Michigan Economic Development Corporation ; Michigan Economic Development Corporation ; Michigan Technology Tri-Corridor ; Michigan Technology Tri-Corridor ; Michigan Technology Tri-Corridor ; Michigan Technology Tri-Corridor ; National Institutes of Health ; National Institutes of Health ; National Institutes of Health ; National Institutes of Health ; University of Illinois at Chicago ; University of Illinois at Chicago ; University of Illinois at Chicago ; University of Illinois at Chicago ; US Department of Energy ; US Department of Energy ; US Department of Energy ; US Department of Energy ; National Institute of General Medical Sciences of the National Institutes of Health ; National Institute of General Medical Sciences of the National Institutes of Health ; National Institute of General Medical Sciences of the National Institutes of Health ; National Institute of General Medical Sciences of the National Institutes of Health ; Michigan Economic Development Corporation ; Michigan Economic Development Corporation ; Michigan Economic Development Corporation ; Michigan Economic Development Corporation ; Michigan Technology Tri-Corridor ; Michigan Technology Tri-Corridor ; Michigan Technology Tri-Corridor ; Michigan Technology Tri-Corridor ; National Institutes of Health ; National Institutes of Health ; National Institutes of Health ; National Institutes of Health ; University of Illinois at Chicago ; University of Illinois at Chicago ; University of Illinois at Chicago ; University of Illinois at Chicago
Indexed BySCI ; SCI
Language英语
Funding ProjectUS Department of Energy[DE-AC02-06CH11357] ; National Institute of General Medical Sciences of the National Institutes of Health[R24GM111072] ; Michigan Economic Development Corporation ; Michigan Technology Tri-Corridor[085P1000817] ; National Institutes of Health[R01EY024363] ; University of Illinois at Chicago
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics
WOS SubjectBiochemical Research Methods ; Chemistry, Multidisciplinary ; Chemistry, Analytical ; Nanoscience & Nanotechnology
WOS IDWOS:000448901700010
WOS KeywordX-RAY-DIFFRACTION ; FREE-ELECTRON LASERS ; MACROMOLECULAR CRYSTALLOGRAPHY ; FEMTOSECOND CRYSTALLOGRAPHY ; PROTEIN CRYSTALLIZATION ; LAUE DIFFRACTION ; 6-4 PHOTOLYASE ; PLATFORM ; DEVICE ; RATES
PublisherROYAL SOC CHEMISTRY
Funding OrganizationUS Department of Energy ; US Department of Energy ; US Department of Energy ; US Department of Energy ; National Institute of General Medical Sciences of the National Institutes of Health ; National Institute of General Medical Sciences of the National Institutes of Health ; National Institute of General Medical Sciences of the National Institutes of Health ; National Institute of General Medical Sciences of the National Institutes of Health ; Michigan Economic Development Corporation ; Michigan Economic Development Corporation ; Michigan Economic Development Corporation ; Michigan Economic Development Corporation ; Michigan Technology Tri-Corridor ; Michigan Technology Tri-Corridor ; Michigan Technology Tri-Corridor ; Michigan Technology Tri-Corridor ; National Institutes of Health ; National Institutes of Health ; National Institutes of Health ; National Institutes of Health ; University of Illinois at Chicago ; University of Illinois at Chicago ; University of Illinois at Chicago ; University of Illinois at Chicago ; US Department of Energy ; US Department of Energy ; US Department of Energy ; US Department of Energy ; National Institute of General Medical Sciences of the National Institutes of Health ; National Institute of General Medical Sciences of the National Institutes of Health ; National Institute of General Medical Sciences of the National Institutes of Health ; National Institute of General Medical Sciences of the National Institutes of Health ; Michigan Economic Development Corporation ; Michigan Economic Development Corporation ; Michigan Economic Development Corporation ; Michigan Economic Development Corporation ; Michigan Technology Tri-Corridor ; Michigan Technology Tri-Corridor ; Michigan Technology Tri-Corridor ; Michigan Technology Tri-Corridor ; National Institutes of Health ; National Institutes of Health ; National Institutes of Health ; National Institutes of Health ; University of Illinois at Chicago ; University of Illinois at Chicago ; University of Illinois at Chicago ; University of Illinois at Chicago ; US Department of Energy ; US Department of Energy ; US Department of Energy ; US Department of Energy ; National Institute of General Medical Sciences of the National Institutes of Health ; National Institute of General Medical Sciences of the National Institutes of Health ; National Institute of General Medical Sciences of the National Institutes of Health ; National Institute of General Medical Sciences of the National Institutes of Health ; Michigan Economic Development Corporation ; Michigan Economic Development Corporation ; Michigan Economic Development Corporation ; Michigan Economic Development Corporation ; Michigan Technology Tri-Corridor ; Michigan Technology Tri-Corridor ; Michigan Technology Tri-Corridor ; Michigan Technology Tri-Corridor ; National Institutes of Health ; National Institutes of Health ; National Institutes of Health ; National Institutes of Health ; University of Illinois at Chicago ; University of Illinois at Chicago ; University of Illinois at Chicago ; University of Illinois at Chicago ; US Department of Energy ; US Department of Energy ; US Department of Energy ; US Department of Energy ; National Institute of General Medical Sciences of the National Institutes of Health ; National Institute of General Medical Sciences of the National Institutes of Health ; National Institute of General Medical Sciences of the National Institutes of Health ; National Institute of General Medical Sciences of the National Institutes of Health ; Michigan Economic Development Corporation ; Michigan Economic Development Corporation ; Michigan Economic Development Corporation ; Michigan Economic Development Corporation ; Michigan Technology Tri-Corridor ; Michigan Technology Tri-Corridor ; Michigan Technology Tri-Corridor ; Michigan Technology Tri-Corridor ; National Institutes of Health ; National Institutes of Health ; National Institutes of Health ; National Institutes of Health ; University of Illinois at Chicago ; University of Illinois at Chicago ; University of Illinois at Chicago ; University of Illinois at Chicago
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/29146
Collection其他_期刊论文
Corresponding AuthorRen, Zhong; Yang, Xiaojing
Affiliation1.Univ Illinois, Dept Chem, Chicago, IL 60607 USA
2.Univ Illinois, Dept Ophthalmol & Vis Sci, Chicago, IL 60607 USA
3.Renz Res Inc, Westmont, IL 60559 USA
4.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Ren, Zhong,Ayhan, Medine,Bandara, Sepatika,et al. Crystal-on-crystal chips for in situ serial diffraction at room temperature[J]. LAB ON A CHIP,2018,18(15):2246-2256.
APA Ren, Zhong.,Ayhan, Medine.,Bandara, Sepatika.,Bowatte, Katinga.,Kumarapperuma, Indika.,...&Yang, Xiaojing.(2018).Crystal-on-crystal chips for in situ serial diffraction at room temperature.LAB ON A CHIP,18(15),2246-2256.
MLA Ren, Zhong,et al."Crystal-on-crystal chips for in situ serial diffraction at room temperature".LAB ON A CHIP 18.15(2018):2246-2256.
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