IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
ITGB1b-Deficient Rare Minnows Delay Grass Carp Reovirus (GCRV) Entry and Attenuate GCRV-Triggered Apoptosis
Chen, Geng1,2; Xiong, Lv1,2; Wang, Yumeng1,3; He, Libo1; Huang, Rong1; Liao, Lanjie1; Zhu, Zuoyan1; Wang, Yaping1
Corresponding AuthorWang, Yaping(wangyp@ihb.ac.cn)
2018-10-01
Source PublicationINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN1422-0067
Volume19Issue:10Pages:18
AbstractIntegrin -1 (ITGB1) is a transmembrane protein belonging to the integrin family and it plays an important role in viral entry. In this study, the itgb1b gene of the rare minnow, Gobiocypris rarus, was cloned and analyzed. To investigate the possible role of itgb1b on grass carp reovirus (GCRV) infection, we generated an ITGB1b-deficient rare minnow (ITGB1b(-/-)) using the CRISPR/Cas9 system. Following stimulation with GCRV, the survival time of the -ITGB1b(-/-) rare minnows was extended in comparison to the wild-type minnows. Moreover, the relative copy number of GCRV and the level of clathrin-mediated endocytosis-associated and apoptosis-related gene expression in the ITGB1b(-/-) rare minnows was significantly lower than that of the wild-type minnows. These results suggested that the absence of itgb1b reduced viral entry efficiency and the expression of apoptosis-related genes. Moreover, the data suggested that itgb1b played an important role in mediating the entry of viruses into the cells via clathrin. Therefore, these findings provide novel insight into the function of itgb1b in the process of GCRV infection.
Keywordgrass carp grass carp reovirus integrin endocytosis clathrin apoptosis
DOI10.3390/ijms19103175
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
Indexed BySCI ; SCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31721005] ; National Natural Science Foundation of China[31702332]
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS IDWOS:000448951000330
WOS KeywordMOUTH-DISEASE VIRUS ; MAMMALIAN REOVIRUS ; CELL ENTRY ; INTEGRIN ; RECEPTOR ; INFECTION ; CORECEPTOR ; TRANSDUCTION ; EXPRESSION ; REQUIRES
PublisherMDPI
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/29118
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorWang, Yaping
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430074, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 101408, Peoples R China
3.Wuhan Univ, Coll Life Sci, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Chen, Geng,Xiong, Lv,Wang, Yumeng,et al. ITGB1b-Deficient Rare Minnows Delay Grass Carp Reovirus (GCRV) Entry and Attenuate GCRV-Triggered Apoptosis[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2018,19(10):18.
APA Chen, Geng.,Xiong, Lv.,Wang, Yumeng.,He, Libo.,Huang, Rong.,...&Wang, Yaping.(2018).ITGB1b-Deficient Rare Minnows Delay Grass Carp Reovirus (GCRV) Entry and Attenuate GCRV-Triggered Apoptosis.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,19(10),18.
MLA Chen, Geng,et al."ITGB1b-Deficient Rare Minnows Delay Grass Carp Reovirus (GCRV) Entry and Attenuate GCRV-Triggered Apoptosis".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 19.10(2018):18.
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