IHB OpenIR  > 其他  > 期刊论文
Identification and characterization of the interferon-gamma-inducible lysosomal thiol reductase gene in Chinese soft-shelled turtle, Pelodiscus sinensis
Fu, Jianping1; Chen, Shannan2; Zhao, Xin1; Luo, Zhang2; Zou, Pengfei2; Liu, Yi1
Corresponding AuthorLiu, Yi(yiliusan@jxnu.edu.cn)
2019
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume90Pages:55-59
AbstractThe reduction of disulfide bonds of exogenous antigens is crucial to the MHC-II class antigen processing and presenting pathway and is catalysed by interferon-gamma-inducible lysosomal thiol reductase (GILT). In this study, a reptile GILT gene from Chinese soft-shelled turtle, Pelodiscus sinensis (PsGILT), was identified. The full-length cDNA of PsGILT is 1631 nucleotides (nt), including a 5'-untranslated region (UTR) of 3 nt, a 3'-UTR of 860 nt and an open reading frame (ORF) of 768 nt encoding 255 amino acids (aa). The conserved features in known GILTs, such as signal peptide, CXXC motif, GILT signature sequence, N-glycosylation site and conserved cysteines, were all found in the putative PsGILT protein. Genomic analysis revealed that PsGILT kept the "7 exons and 6 introns" structure of vertebrate GILT genes. PsGILT was expressed in all examined organs/tissues and was mainly expressed in spleen and blood. Increased mRNA expression levels of PsIFN-gamma and PsGILT in PBLs were observed after induction with LPS, PolyI:C and recombinant IFN-gamma (rIFN-gamma). We also tested the reductase activity of rGILT in vitro and found that it could reduce intact human IgG into H chains and L chains. These above results implied that PsGILT may play an important role in resisting bacterial and viral infections, like other vertebrate GILTs.
KeywordGILT Thiol reductase activity IFN-gamma Pelodiscus sinensis
DOI10.1016/j.dci.2018.08.019
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Jiangxi Province ; Natural Science Foundation of Jiangxi Province ; Natural Science Foundation of Jiangxi Province ; Natural Science Foundation of Jiangxi Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Jiangxi Province ; Natural Science Foundation of Jiangxi Province ; Natural Science Foundation of Jiangxi Province ; Natural Science Foundation of Jiangxi Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Jiangxi Province ; Natural Science Foundation of Jiangxi Province ; Natural Science Foundation of Jiangxi Province ; Natural Science Foundation of Jiangxi Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Jiangxi Province ; Natural Science Foundation of Jiangxi Province ; Natural Science Foundation of Jiangxi Province ; Natural Science Foundation of Jiangxi Province
Indexed BySCI ; SCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31502196] ; Natural Science Foundation of Jiangxi Province[20151BAB214021]
WOS Research AreaFisheries ; Immunology ; Zoology
WOS SubjectFisheries ; Immunology ; Zoology
WOS IDWOS:000449130300007
WOS KeywordINNATE IMMUNE-RESPONSE ; EXPRESSION ANALYSIS ; GILT GENE ; MOLECULAR-CLONING ; DISULFIDE BONDS ; IFN-GAMMA ; REPLICATION ; MATURATION
PublisherELSEVIER SCI LTD
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Jiangxi Province ; Natural Science Foundation of Jiangxi Province ; Natural Science Foundation of Jiangxi Province ; Natural Science Foundation of Jiangxi Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Jiangxi Province ; Natural Science Foundation of Jiangxi Province ; Natural Science Foundation of Jiangxi Province ; Natural Science Foundation of Jiangxi Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Jiangxi Province ; Natural Science Foundation of Jiangxi Province ; Natural Science Foundation of Jiangxi Province ; Natural Science Foundation of Jiangxi Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Jiangxi Province ; Natural Science Foundation of Jiangxi Province ; Natural Science Foundation of Jiangxi Province ; Natural Science Foundation of Jiangxi Province
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/29050
Collection其他_期刊论文
Corresponding AuthorLiu, Yi
Affiliation1.Jiangxi Normal Univ, Coll Life Sci, 99 Ziyang Rd, Nanchang 330022, Jiangxi, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Fu, Jianping,Chen, Shannan,Zhao, Xin,et al. Identification and characterization of the interferon-gamma-inducible lysosomal thiol reductase gene in Chinese soft-shelled turtle, Pelodiscus sinensis[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2019,90:55-59.
APA Fu, Jianping,Chen, Shannan,Zhao, Xin,Luo, Zhang,Zou, Pengfei,&Liu, Yi.(2019).Identification and characterization of the interferon-gamma-inducible lysosomal thiol reductase gene in Chinese soft-shelled turtle, Pelodiscus sinensis.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,90,55-59.
MLA Fu, Jianping,et al."Identification and characterization of the interferon-gamma-inducible lysosomal thiol reductase gene in Chinese soft-shelled turtle, Pelodiscus sinensis".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 90(2019):55-59.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Fu, Jianping]'s Articles
[Chen, Shannan]'s Articles
[Zhao, Xin]'s Articles
Baidu academic
Similar articles in Baidu academic
[Fu, Jianping]'s Articles
[Chen, Shannan]'s Articles
[Zhao, Xin]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Fu, Jianping]'s Articles
[Chen, Shannan]'s Articles
[Zhao, Xin]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.