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Depletion of Tissue-Specific Ion Transporters Causes Differential Expression of PRL Targets in Response to Increased Levels of Endogenous PRL
Shu, Tingting1,2; Shu, Yuqin1,2; Gao, Yanping1,2; Jin, Xia1; He, Jiangyan1; Zhai, Gang1; Yin, Zhan1
Corresponding AuthorZhai, Gang(zhaigang@ihb.ac.cn) ; Yin, Zhan(zyin@ihb.ac.cn)
2018-11-20
Source PublicationFRONTIERS IN ENDOCRINOLOGY
ISSN1664-2392
Volume9Pages:14
AbstractProlactin (PRL) has been considered a key regulator of ion uptake in zebrafish. The genes slc12a10.2 and slc12a3, which are Na+ and chloride Cl- co-transporters, have been reported to be regulated by PRL in freshwater fish. The integrative network of PRL signaling dissected from the knockout of tissue-specific downstream PRL ion transporters remains poor. In the present study, zebrafish models with increased endogenous levels of PRL were generated through the knockout of slc12a10.2 or slc12a3, and the developmental consequences were analyzed. The increased levels of pituitary PRL were observed in both slc12a10.2- and slc12a3-deficient fish. Unlike the slc12a3-deficient fish, which could survive to adulthood, the slc12a10.2-deficient fish began to die at 9 days post-fertilization (dpf) and did not survive beyond 17 dpf. This survival defect is a result of defective Cl- uptake in this mutant, indicating that Slc12a10.2 plays an essential role in Cl- uptake. Intriguingly, compared to the levels in control fish, no significant differences in the levels of Na+ in the body were observed in slc12a10.2- or slc12a3-deficient zebrafish. The upregulations of the PRL downstream transporters, slc9a3.2, slc12a10.2, and atp1a1a.5 were observed in slc12a3-deficient fish in both the gills/skin and the pronephric duct. However, this type of response was not observed in the pronephric duct of slc12a10.2-deficient fish, except under Na+-deprived conditions. Our results show that PRL is susceptible to deficiencies in downstream ion transporters. Moreover, both the gills/skin and pronephric duct show differential expression of downstream PRL targets in response to increased levels of pituitary PRL caused by the depletion of tissue-specific ion transporters.
Keywordprolactin ion transporter gills/skin pronephric duct slc12a10.2 slc12a3
DOI10.3389/fendo.2018.00683
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China (973 Program) ; National Basic Research Program of China (973 Program) ; National Basic Research Program of China (973 Program) ; National Basic Research Program of China (973 Program) ; Pilot Program A Project from the Chinese Academy of Sciences ; Pilot Program A Project from the Chinese Academy of Sciences ; Pilot Program A Project from the Chinese Academy of Sciences ; Pilot Program A Project from the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China (973 Program) ; National Basic Research Program of China (973 Program) ; National Basic Research Program of China (973 Program) ; National Basic Research Program of China (973 Program) ; Pilot Program A Project from the Chinese Academy of Sciences ; Pilot Program A Project from the Chinese Academy of Sciences ; Pilot Program A Project from the Chinese Academy of Sciences ; Pilot Program A Project from the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China (973 Program) ; National Basic Research Program of China (973 Program) ; National Basic Research Program of China (973 Program) ; National Basic Research Program of China (973 Program) ; Pilot Program A Project from the Chinese Academy of Sciences ; Pilot Program A Project from the Chinese Academy of Sciences ; Pilot Program A Project from the Chinese Academy of Sciences ; Pilot Program A Project from the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China (973 Program) ; National Basic Research Program of China (973 Program) ; National Basic Research Program of China (973 Program) ; National Basic Research Program of China (973 Program) ; Pilot Program A Project from the Chinese Academy of Sciences ; Pilot Program A Project from the Chinese Academy of Sciences ; Pilot Program A Project from the Chinese Academy of Sciences ; Pilot Program A Project from the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences
Indexed BySCI ; SCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31501857] ; National Natural Science Foundation of China[31530077] ; National Basic Research Program of China (973 Program)[2014CB138602] ; Pilot Program A Project from the Chinese Academy of Sciences[XDA08010405] ; Knowledge Innovation Program of the Chinese Academy of Sciences
WOS Research AreaEndocrinology & Metabolism
WOS SubjectEndocrinology & Metabolism
WOS IDWOS:000450701600001
WOS KeywordTILAPIA OREOCHROMIS-NILOTICUS ; SIGNAL-TRANSDUCTION PATHWAYS ; ZEBRAFISH DANIO-RERIO ; PROLACTIN RECEPTOR ; CHLORIDE CHANNEL ; GROWTH-HORMONE ; FRESH-WATER ; EMBRYONIC-DEVELOPMENT ; NA+/CL-COTRANSPORTER ; FISH OSMOREGULATION
PublisherFRONTIERS MEDIA SA
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China (973 Program) ; National Basic Research Program of China (973 Program) ; National Basic Research Program of China (973 Program) ; National Basic Research Program of China (973 Program) ; Pilot Program A Project from the Chinese Academy of Sciences ; Pilot Program A Project from the Chinese Academy of Sciences ; Pilot Program A Project from the Chinese Academy of Sciences ; Pilot Program A Project from the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China (973 Program) ; National Basic Research Program of China (973 Program) ; National Basic Research Program of China (973 Program) ; National Basic Research Program of China (973 Program) ; Pilot Program A Project from the Chinese Academy of Sciences ; Pilot Program A Project from the Chinese Academy of Sciences ; Pilot Program A Project from the Chinese Academy of Sciences ; Pilot Program A Project from the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China (973 Program) ; National Basic Research Program of China (973 Program) ; National Basic Research Program of China (973 Program) ; National Basic Research Program of China (973 Program) ; Pilot Program A Project from the Chinese Academy of Sciences ; Pilot Program A Project from the Chinese Academy of Sciences ; Pilot Program A Project from the Chinese Academy of Sciences ; Pilot Program A Project from the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program of China (973 Program) ; National Basic Research Program of China (973 Program) ; National Basic Research Program of China (973 Program) ; National Basic Research Program of China (973 Program) ; Pilot Program A Project from the Chinese Academy of Sciences ; Pilot Program A Project from the Chinese Academy of Sciences ; Pilot Program A Project from the Chinese Academy of Sciences ; Pilot Program A Project from the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences ; Knowledge Innovation Program of the Chinese Academy of Sciences
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/29044
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorZhai, Gang; Yin, Zhan
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Coll Adv Agr Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Shu, Tingting,Shu, Yuqin,Gao, Yanping,et al. Depletion of Tissue-Specific Ion Transporters Causes Differential Expression of PRL Targets in Response to Increased Levels of Endogenous PRL[J]. FRONTIERS IN ENDOCRINOLOGY,2018,9:14.
APA Shu, Tingting.,Shu, Yuqin.,Gao, Yanping.,Jin, Xia.,He, Jiangyan.,...&Yin, Zhan.(2018).Depletion of Tissue-Specific Ion Transporters Causes Differential Expression of PRL Targets in Response to Increased Levels of Endogenous PRL.FRONTIERS IN ENDOCRINOLOGY,9,14.
MLA Shu, Tingting,et al."Depletion of Tissue-Specific Ion Transporters Causes Differential Expression of PRL Targets in Response to Increased Levels of Endogenous PRL".FRONTIERS IN ENDOCRINOLOGY 9(2018):14.
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