| Dietary citric acid supplementation alleviates soybean meal-induced intestinal oxidative damage and micro-ecological imbalance in juvenile turbot, Scophthalmus maximus L | |
| Chen, Zhichu1,2; Zhao, Sifan1,2; Liu, Yang1,2; Yang, Pei1,2; Ai, Qinghui1,2; Zhang, Wenbing1,2; Xu, Weiqi1,2; Zhang, Yongan3; Zhang, Yanjiao1,2,4; Mai, Kangsen1,2,4 | |
| Corresponding Author | Zhang, Yanjiao(yanjiaozhang@ouc.edu.cn) |
| 2018-12-01 | |
| Source Publication | AQUACULTURE RESEARCH
 |
| ISSN | 1355-557X |
| Volume | 49Issue:12Pages:3804-3816 |
| Abstract | A 12-week feeding trial was conducted to investigate the potential protective effects of citric acid against soybean meal-induced intestinal oxidative damage and micro-ecological imbalance in juvenile turbot (S. maximus L.). Four isonitrogenous and isolipidic experimental diets, that is fish meal-based diet (FM), FM with 40% fish meal protein replaced with soybean meal protein diet (SBM), supplemented with 1.5% citric acid (1.5% CA) or 3% citric acid (3% CA). Results showed that both citric acid diets significantly enhanced the total antioxidative capacity and the gene expression of superoxide dismutase, glutathione peroxidase and heme oxygenase 1, while also decreasing the malondialdehyde content in the distal intestine. Compared to diet SBM, the genes expression of p53, protein kinase C and Caspase-3 were remarkably declined by dietary citric acid supplementation, while the genes expression of proliferating cell nuclear antigen and mucins showed an opposite trend. The structural integrity of the distal intestine in fish fed citric acid was showed in the histological results. Sequencing of bacterial 16s rRNA V4 region showed that the profile of intestinal bacteria was altered by dietary citric acid supplementation, which was supported by the diet-cluster of PCoA and phylogenetic tree. MetaStat analysis indicated that dietary citric acid dramatically reduced the relative abundance of the Vibrio genus. In conclusion, dietary citric acid mitigated soybean meal-induced intestinal oxidative damage, beneficially alleviated the micro-ecological imbalance and specifically reduced the relative abundance of the Vibrio genus in the distal intestine of juvenile turbot. |
| Keyword | citric acid intestinal barrier function oxidative stress soybean meal turbot |
| DOI | 10.1111/are.13847 |
| Funding Organization | National Basic Research Program ; National Basic Research Program ; National Basic Research Program ; National Basic Research Program ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program ; National Basic Research Program ; National Basic Research Program ; National Basic Research Program ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program ; National Basic Research Program ; National Basic Research Program ; National Basic Research Program ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program ; National Basic Research Program ; National Basic Research Program ; National Basic Research Program ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China |
| Indexed By | SCI ; SCI |
| Language | 英语 |
| Funding Project | National Basic Research Program[2014CB138601] ; China Agriculture Research System[CARS 50-G08] ; National Natural Science Foundation of China[41576137] ; National Natural Science Foundation of China[31202006] |
| WOS Research Area | Fisheries |
| WOS Subject | Fisheries |
| WOS ID | WOS:000449876500011 |
| WOS Keyword | DIGESTIVE ENZYME-ACTIVITIES ; SHRIMP LITOPENAEUS-VANNAMEI ; AIRWAY EPITHELIAL-CELLS ; TILAPIA OREOCHROMIS SP. ; CHAIN FATTY-ACIDS ; SALMO-SALAR L. ; GROWTH-PERFORMANCE ; ORGANIC-ACIDS ; GUT MICROBIOTA ; HOST-DEFENSE |
| Publisher | WILEY |
| Funding Organization | National Basic Research Program ; National Basic Research Program ; National Basic Research Program ; National Basic Research Program ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program ; National Basic Research Program ; National Basic Research Program ; National Basic Research Program ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program ; National Basic Research Program ; National Basic Research Program ; National Basic Research Program ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Program ; National Basic Research Program ; National Basic Research Program ; National Basic Research Program ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/29028 |
| Collection | 水生生物分子与细胞生物学研究中心_期刊论文 |
| Corresponding Author | Zhang, Yanjiao |
| Affiliation | 1.Ocean Univ China, Minist Agr, Key Lab Aquaculture Nutr & Feed, Qingdao, Peoples R China 2.Ocean Univ China, Minist Educ, Key Lab Mariculture, Qingdao, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Hubei, Peoples R China 4.Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Peoples R China |
| Recommended Citation GB/T 7714 | Chen, Zhichu,Zhao, Sifan,Liu, Yang,et al. Dietary citric acid supplementation alleviates soybean meal-induced intestinal oxidative damage and micro-ecological imbalance in juvenile turbot, Scophthalmus maximus L[J]. AQUACULTURE RESEARCH,2018,49(12):3804-3816. |
| APA | Chen, Zhichu.,Zhao, Sifan.,Liu, Yang.,Yang, Pei.,Ai, Qinghui.,...&Mai, Kangsen.(2018).Dietary citric acid supplementation alleviates soybean meal-induced intestinal oxidative damage and micro-ecological imbalance in juvenile turbot, Scophthalmus maximus L.AQUACULTURE RESEARCH,49(12),3804-3816. |
| MLA | Chen, Zhichu,et al."Dietary citric acid supplementation alleviates soybean meal-induced intestinal oxidative damage and micro-ecological imbalance in juvenile turbot, Scophthalmus maximus L".AQUACULTURE RESEARCH 49.12(2018):3804-3816. |
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