| Oocyte-specific maternal Slbp2 is required for replication-dependent histone storage and early nuclear cleavage in zebrafish oogenesis and embryogenesis | |
| He, Wen-Xia1,2; Wu, Min1,2; Liu, Zhen1,2; Li, Zhi1; Wang, Yang1,2; Zhou, Jian1,2; Yu, Peng1,2; Zhang, Xiao-Juan1; Zhou, Li1,2; Gui, Jian-Fang1,2 | |
| Corresponding Author | Zhou, Li(zhouli@ihb.ac.cn) ; Gui, Jian-Fang(jfgui@ihb.ac.cn) |
| 2018-12-01 | |
| Source Publication | RNA
 |
| ISSN | 1355-8382 |
| Volume | 24Issue:12Pages:1738-1748 |
| Abstract | Stem-loop binding protein (SLBP) is required for replication-dependent histone mRNA metabolism in mammals. Zebrafish possesses two slbps, and slbp1 is necessary for retinal neurogenesis. However, the detailed expression and function of slbp2 in zebrafish are still unknown. In this study, we first identified zebrafish slbp2 as an oocyte-specific maternal factor and then generated a maternal-zygotic slbp2F3 homozygous mutant (MZslbp2 Delta 4(-/-)) using CRISPR/Cas9. The depletion of maternal Slbp2 disrupted early nuclear cleavage, which resulted in developmental arrest at the MBT stage. The developmental defects could be rescued in slbp2transgenic MZslbp2 Delta 4(-/-) embryos. However, homozygous mutant MZslbp1 Delta 1(-/-)developed normally, indicating slbp1 is dispensable for zebrafish early embryogenesis. Through comparative proteome and transcriptome profiling between WT and MZslbp2 Delta 4(-/- )embryos, we identified many differentially expressed proteins and genes. In comparison with those in WT embryos, four replication-dependent histones, including H2a, H2b, H3, and H4, all reduced their expression, while histone variant h2afx significantly increased in MZslbp2 Delta 4(-/- )embryos at the 256-cell stage and high stage. Zebrafish Slbp2 can bind histone mRNA stem-loop in vitro, and the defects of MZslbp2 Delta 4(-/-) embryos can be partially rescued by overexpression of H2b. The current data indicate that maternal Slbp2 plays a pivotal role in the storage of replication-dependent histone mRNAs and proteins during zebrafish oogenesis. |
| Keyword | slbp2 replication-dependent histones ribosomal proteins maternal effect oogenesis early embryogenesis |
| DOI | 10.1261/rna.067090.118 |
| Funding Organization | Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology |
| Indexed By | SCI ; SCI |
| Language | 英语 |
| Funding Project | Key Program of Frontier Sciences of the Chinese Academy of Sciences[QYZDY-SSW-SMC025] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA08030201] ; Earmarked Fund for Modern Agro-industry Technology Research System[NYCYTX-49] ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology[2016FBZ01] |
| WOS Research Area | Biochemistry & Molecular Biology |
| WOS Subject | Biochemistry & Molecular Biology |
| WOS ID | WOS:000450373800011 |
| WOS Keyword | LOOP-BINDING-PROTEIN ; EARLY EMBRYONIC-DEVELOPMENT ; STEM-LOOP ; MESSENGER-RNA ; GENE-EXPRESSION ; H2AX PHOSPHORYLATION ; GENOMIC INSTABILITY ; POLY(A) TAIL ; IN-VIVO ; TRANSLATION |
| Publisher | COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT |
| Funding Organization | Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/29022 |
| Collection | 鱼类生物学及渔业生物技术研究中心_期刊论文 |
| Corresponding Author | Zhou, Li; Gui, Jian-Fang |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| Recommended Citation GB/T 7714 | He, Wen-Xia,Wu, Min,Liu, Zhen,et al. Oocyte-specific maternal Slbp2 is required for replication-dependent histone storage and early nuclear cleavage in zebrafish oogenesis and embryogenesis[J]. RNA,2018,24(12):1738-1748. |
| APA | He, Wen-Xia.,Wu, Min.,Liu, Zhen.,Li, Zhi.,Wang, Yang.,...&Gui, Jian-Fang.(2018).Oocyte-specific maternal Slbp2 is required for replication-dependent histone storage and early nuclear cleavage in zebrafish oogenesis and embryogenesis.RNA,24(12),1738-1748. |
| MLA | He, Wen-Xia,et al."Oocyte-specific maternal Slbp2 is required for replication-dependent histone storage and early nuclear cleavage in zebrafish oogenesis and embryogenesis".RNA 24.12(2018):1738-1748. |
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