IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
A novel PDZ domain-containing gene is essential for male sex differentiation and maintenance in yellow catfish (Pelteobagrus fulvidraco)
Dan, Cheng1; Lin, Qiaohong1; Gong, Gaorui1; Yang, Tianyi1; Xiong, Shuting1; Xiong, Yang1; Huang, Peipei2; Gui, Jian-Fang1,2; Mei, Jie1
Corresponding AuthorGui, Jian-Fang(jfgui@ihb.ac.cn) ; Mei, Jie(jmei@mail.hzau.edu.cn)
2018-11-15
Source PublicationSCIENCE BULLETIN
ISSN2095-9273
Volume63Issue:21Pages:1420-1430
AbstractThe sex-determining genes are found to be variable among different fish species. Yellow catfish (Pelteobagrus fulvidraco) is an important aquaculture fish species in China with XX/XY sex-determining type. Recently, YY super-male yellow catfish has been successfully produced by combining hormonal-induced sex reversal method with sex chromosome-linked markers. Here, we identified a novel PDZ domain-containing gene in yellow catfish designated as pfpdz1, in whose intron the sex-linked marker was located. The coding sequence of pfpdz1 in Y chromosome was identical to that in X chromosome except a missense SNP (A/T) that changes an amino acid (E8V) in the N-terminal region. Pfpdz1 displayed male-specific expression during sex differentiation. Overexpression of pfpdz1 using additive transgenesis induces XX ovary to differentiate into testis-like tissue, while the targeted inactivation of pfpdz1 in Y chromosome using CRISPR/Cas9-mediated mutagenesis triggers ovarian differentiation. Furthermore, we demonstrated that pfpdz1 initiates testicular differentiation through upregulating expression of amh, dmrt1 and sox9a1, as well as downregulating expression of cyp19a1, foxl2 and wnt4. Our data provide functional evidence that pfpdz1 is significant for male differentiation and maintenance in yellow catfish. (C) 2018 Science China Press. Published by Elsevier B.V. and Science China Press. All rights reserved.
KeywordSex differentiation Yellow catfish Pfpdz1 Sex reversal
DOI10.1016/j.scib.2018.08.012
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Agricultural Research System ; China Agricultural Research System ; China Agricultural Research System ; China Agricultural Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Agricultural Research System ; China Agricultural Research System ; China Agricultural Research System ; China Agricultural Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Agricultural Research System ; China Agricultural Research System ; China Agricultural Research System ; China Agricultural Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Agricultural Research System ; China Agricultural Research System ; China Agricultural Research System ; China Agricultural Research System
Indexed BySCI ; SCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31672635] ; China Agricultural Research System[CARS-46]
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000451636900007
WOS KeywordDMRT1 ; MARKERS ; TILAPIA ; CHROMOSOME ; REVERSAL ; IDENTIFICATION ; EFFICIENT ; ESTROGEN ; MEDAKA ; ROLES
PublisherSCIENCE PRESS
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Agricultural Research System ; China Agricultural Research System ; China Agricultural Research System ; China Agricultural Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Agricultural Research System ; China Agricultural Research System ; China Agricultural Research System ; China Agricultural Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Agricultural Research System ; China Agricultural Research System ; China Agricultural Research System ; China Agricultural Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Agricultural Research System ; China Agricultural Research System ; China Agricultural Research System ; China Agricultural Research System
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Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/29000
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorGui, Jian-Fang; Mei, Jie
Affiliation1.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Dan, Cheng,Lin, Qiaohong,Gong, Gaorui,et al. A novel PDZ domain-containing gene is essential for male sex differentiation and maintenance in yellow catfish (Pelteobagrus fulvidraco)[J]. SCIENCE BULLETIN,2018,63(21):1420-1430.
APA Dan, Cheng.,Lin, Qiaohong.,Gong, Gaorui.,Yang, Tianyi.,Xiong, Shuting.,...&Mei, Jie.(2018).A novel PDZ domain-containing gene is essential for male sex differentiation and maintenance in yellow catfish (Pelteobagrus fulvidraco).SCIENCE BULLETIN,63(21),1420-1430.
MLA Dan, Cheng,et al."A novel PDZ domain-containing gene is essential for male sex differentiation and maintenance in yellow catfish (Pelteobagrus fulvidraco)".SCIENCE BULLETIN 63.21(2018):1420-1430.
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