IHB OpenIR  > 水生生物多样性与资源保护研究中心  > 期刊论文
Isolation and characterization of microsatellite markers in a highland fish, Pareuchiloglanis sinensis (Siluriformes: Sisoridae) by next-generation sequencing
Chen, Weitao1,2; He, Shunping1
Corresponding AuthorHe, Shunping(clad@ihb.ac.cn)
2018-12-01
Source PublicationJOURNAL OF GENETICS
ISSN0022-1333
Volume97Pages:111-116
AbstractPareuchiloglanis sinensis (Siluriformes: Sisoridae) is an endemic and highland fish species which occurs only in some rivers of south-west China. In this study, the isolation and characterization of polymorphic microsatellite loci of this fish species by next-generation sequencing is described. A total of 9471 simple-sequence repeats (SSRs) were observed from RNA-seq data. One hundred and twenty primer pairs were chosen randomly and validated across 48 P. sinensis individuals collected from the Dadu river (a branch of the Yangtze river) of which 28 polymorphic microsatellite loci were detected. The number of alleles ranged from 2 to 14, with an average of seven alleles per locus. Twenty loci exhibited high polymorphism with the polymorphism information content (PIC) larger than 0.5. The mean observed and expected heterozygosity varied from 0.104 to 0.958 and 0.157 to 0.844, with an average of 0.583 and 0.613, respectively. The microsatellite markers characterized in the current study serve as a useful tool for the conversation genetic studies and population evaluation of P. sinensis.
Keywordmicrosatellite markers Dadu river RNA-seq Pareuchiloglanis sinensis
DOI10.1007/s12041-018-0997-6
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
Indexed BySCI ; SCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31130049] ; National Natural Science Foundation of China[U1036603]
WOS Research AreaGenetics & Heredity
WOS SubjectGenetics & Heredity
WOS IDWOS:000451438500014
PublisherINDIAN ACAD SCIENCES
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/28986
Collection水生生物多样性与资源保护研究中心_期刊论文
Corresponding AuthorHe, Shunping
Affiliation1.Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
2.CAFS, Pearl River Fisheries Res Inst, Guangzhou 510380, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Chen, Weitao,He, Shunping. Isolation and characterization of microsatellite markers in a highland fish, Pareuchiloglanis sinensis (Siluriformes: Sisoridae) by next-generation sequencing[J]. JOURNAL OF GENETICS,2018,97:111-116.
APA Chen, Weitao,&He, Shunping.(2018).Isolation and characterization of microsatellite markers in a highland fish, Pareuchiloglanis sinensis (Siluriformes: Sisoridae) by next-generation sequencing.JOURNAL OF GENETICS,97,111-116.
MLA Chen, Weitao,et al."Isolation and characterization of microsatellite markers in a highland fish, Pareuchiloglanis sinensis (Siluriformes: Sisoridae) by next-generation sequencing".JOURNAL OF GENETICS 97(2018):111-116.
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