Androgen signaling regulates the transcription of anti-Mullerian hormone via synergy with SRY-related protein SOX9A
Zhai, Gang1; Shu, Tingting1,2; Xia, Yuguo3; Jin, Xia1; He, Jiangyan1; Yin, Zhan1
2017-02-15
Source PublicationSCIENCE BULLETIN
ISSN2095-9273
Volume62Issue:3Pages:197-203
AbstractAnti-Mullerian hormone (amh) is one of the earliest functional genes expressed during testicular differentiation. It has been suggested that androgen signaling regulates critical genes for the differentiation and development of the testis. To elucidate the exact regulatory mechanisms involved in amh transcription mediated by androgen signaling, androgen signaling was manipulated in zebrafish by cytochrome P450 17a1 (cyp17a1) knockout and Flutamide treatment. In cyp17a1-deficient and Flutamide-treated testes, up-regulated sry-box9a (sox9a) and down-regulated amh were observed. Moreover, a physical association of the zebrafish androgen receptor (AR) and SOX9A was found. The interaction between AR and SOX9A was mediated via the DNA binding domain (DBD) of AR and the transactivation domain (TA) of SOX9A, and was further enhanced by 5-alpha dihydrotestosterone (DHT), one of the most potent androgens. Intriguingly, together with SOX9A, androgen signaling synergistically promoted amh transcription, mainly through the proximal 1 kb of the amh promoter region. Taken together, our data demonstrate a critical mechanism underlying the direct synergy of androgen signaling and SOX9A in the regulation of amh transcription. (C) 2017 Science China Press. Published by Elsevier B.V. and Science China Press. All rights reserved.
SubtypeArticle
KeywordZebrafish Androgen Signaling Sox9a Transcription Amh
DOI10.1016/j.scib.2017.01.007
WOS HeadingsScience & Technology
Indexed BySCI
Funding OrganizationNational Natural Science Foundation of China(31501857 ; National Natural Science Foundation of China(31501857 ; National Basic Research Program of China(2014CB138602) ; National Basic Research Program of China(2014CB138602) ; 31530077) ; 31530077) ; National Natural Science Foundation of China(31501857 ; National Natural Science Foundation of China(31501857 ; National Basic Research Program of China(2014CB138602) ; National Basic Research Program of China(2014CB138602) ; 31530077) ; 31530077)
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS KeywordGENE-EXPRESSION ; COMPLEMENTARY-DNA ; PROSTATE-CANCER ; RECEPTOR ; ZEBRAFISH ; RAT ; SPERMATOGENESIS ; EXPOSURE ; CLONING ; TESTIS
WOS IDWOS:000396758000007
Funding OrganizationNational Natural Science Foundation of China(31501857 ; National Natural Science Foundation of China(31501857 ; National Basic Research Program of China(2014CB138602) ; National Basic Research Program of China(2014CB138602) ; 31530077) ; 31530077) ; National Natural Science Foundation of China(31501857 ; National Natural Science Foundation of China(31501857 ; National Basic Research Program of China(2014CB138602) ; National Basic Research Program of China(2014CB138602) ; 31530077) ; 31530077)
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/28968
Collection藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou 510380, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Zhai, Gang,Shu, Tingting,Xia, Yuguo,et al. Androgen signaling regulates the transcription of anti-Mullerian hormone via synergy with SRY-related protein SOX9A[J]. SCIENCE BULLETIN,2017,62(3):197-203.
APA Zhai, Gang,Shu, Tingting,Xia, Yuguo,Jin, Xia,He, Jiangyan,&Yin, Zhan.(2017).Androgen signaling regulates the transcription of anti-Mullerian hormone via synergy with SRY-related protein SOX9A.SCIENCE BULLETIN,62(3),197-203.
MLA Zhai, Gang,et al."Androgen signaling regulates the transcription of anti-Mullerian hormone via synergy with SRY-related protein SOX9A".SCIENCE BULLETIN 62.3(2017):197-203.
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