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题名: Polyphenolic allelochemical pyrogallic acid induces caspase-3(like)-dependent programmed cell death in the cyanobacterium Microcystis aeruginosa
作者: Lu, Zhiying1, 2; Sha, Jun1, 2; Tian, Yun3; Zhang, Xuezhi1, 2; Liu, Biyun3; Wu, Zhenbin3
关键词: Allelopathic interaction ; Caspase ; Sell death ; DNA fragmentation ; Pyrogallic acid ; Reactive oxygen species
刊名: ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
发表日期: 2017
DOI: 10.1016/j.algal.2016.11.007
卷: 21, 页:148-155
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology
英文摘要: The naturally occurring polyphenolic allelochemical pyrogallic acid (PA) is secreted by the aquatic vascular plant Myriophyllum spicatum and exhibits strong inhibitory effects on harmful cyanobacteria; however, the mechanisms underlying this inhibitory effect are largely unknown. In the current study, the biochemical and morphological changes that occurred during the PA-induced cell death of Microcystis aeruginosa and the mechanisms underlying these changeswere investigated. PA exposure inhibited the growth ofM. aeruginosa and significantly elevated caspase-3(-like) activities. The caspase-3 inhibitor aldehyde-terminated tetrapeptide (Ac-DEVD-CHO) abrogated the PA-induced caspase-3(-like) activities, which resulted in the alleviation of PA-induced growth inhibition. WhenM. aeruginosa cellswere exposed to 14 mgL(-1) PA for 72 h, nucleoid disintegration, photosynthetic lamellae rupture, pyknosis formation, vacuolation and DNA fragmentation were observed. The alignment of caspase domains showed that the key functional regions were conserved and showed remarkable orthology among different M. aeruginosa strains. Furthermore, a gas chromatography-mass spectrum analysis indicated that PA was gradually degraded. In addition, elevated intracellular reactive oxygen species (ROS) were detected. These results suggested that the production of ROS during the autoxidation of PA might act as a mediator that led to caspase-3(-like) activation and the subsequent initiation of programmed cell death in the cyanobacterium M. aeruginosa. (C) 2016 Elsevier B. V. All rights reserved.
关键词[WOS]: OXIDATIVE STRESS ; MYRIOPHYLLUM-SPICATUM ; UNICELLULAR CHLOROPHYTE ; SUBMERGED MACROPHYTES ; ANNEXIN-V ; APOPTOSIS ; MECHANISM ; SYSTEM ; LAKE ; PHYTOPLANKTON
语种: 英语
项目资助者: National Nature Science Foundation of China(51178452 ; 50909091 ; 31123001)
WOS记录号: WOS:000396365500017
ISSN号: 2211-9264
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/28965
Appears in Collections:水环境工程研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Ctr Microalgal Biotechnol & Biofuels, Wuhan, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
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