IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Deletion of Pr72 causes cardiac developmental defects in Zebrafish
Song, Guibo1; Han, Mingjun1; Li, Zuhua1; Gan, Xuedong2; Chen, Xiaowen3; Yang, Jie1; Dong, Sufang1; Yan, Ming1; Wan, Jun4; Wang, Yanggan2; Huang, Zhuliang1; Yin, Zhan3; Zheng, Fang1
Corresponding AuthorYin, Zhan(zyin@ihb.ac.cn) ; Zheng, Fang(zhengfang@whu.edu.cn)
2018-11-27
Source PublicationPLOS ONE
ISSN1932-6203
Volume13Issue:11Pages:12
AbstractThe alpha regulator subunit B'' of protein phosphatase 2 (PPP2R3A), a regulatory subunit of protein phosphatase 2A (PP2A), was reported to present a special subcellular localization in cardiomyocytes and elevate in non-ischemia failing hearts. PPP2R3A has two transcriptions PR72 and PR130. PR72 acts as a negative regulator of the Wnt signaling cascade, while the Wnt signaling cascade plays a pivotal role in cardiac development. And PR130 was found to be involved in cardiac development of zebrafish in our previous study. Thus, to investigate the function of PR72 in heart, two stable pr72 knockout (KO) zebrafish lines were generated using Transcription Activator-Like Effector Nuclease (TALEN) technology. Homozygous pr72 KO fish struggled to survive to adulthood and exhibited cardiac developmental defects, including enlarged ventricular chambers, reduced cardiomyocytes and decreased cardiac function. And the defective sarcomere ultrastructure that affected mitochondria, I bands, Z lines, and intercalated disks was also observed. Furthermore, the abnormal heart looping was detected in mutants which could be rescued by injection with wild type pr72 mRNA. Additionally, it was found that Wnt effectors were elevated in mutants. Those indicated that deletion of pr72 in zebrafish interrupted cardiac development, probably through activation of the Wnt pathway.
DOI10.1371/journal.pone.0206883
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
Indexed BySCI ; SCI
Language英语
Funding ProjectNational Natural Science Foundation of China[81472024]
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000451440000007
WOS KeywordPROTEIN PHOSPHATASE 2A ; SERINE/THREONINE PHOSPHATASES ; REGULATORY SUBUNIT ; PHOSPHORYLATION ; PP2A
PublisherPUBLIC LIBRARY SCIENCE
Funding OrganizationNational Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/28964
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorYin, Zhan; Zheng, Fang
Affiliation1.Wuhan Univ, Ctr Gene Diag, Zhongnan Hosp, Wuhan, Hubei, Peoples R China
2.Wuhan Univ, Dept Cardiol, Zhongnan Hosp, Wuhan, Hubei, Peoples R China
3.Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan, Hubei, Peoples R China
4.Wuhan Univ, Dept Cardiol, Renmin Hosp, Wuhan, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Song, Guibo,Han, Mingjun,Li, Zuhua,et al. Deletion of Pr72 causes cardiac developmental defects in Zebrafish[J]. PLOS ONE,2018,13(11):12.
APA Song, Guibo.,Han, Mingjun.,Li, Zuhua.,Gan, Xuedong.,Chen, Xiaowen.,...&Zheng, Fang.(2018).Deletion of Pr72 causes cardiac developmental defects in Zebrafish.PLOS ONE,13(11),12.
MLA Song, Guibo,et al."Deletion of Pr72 causes cardiac developmental defects in Zebrafish".PLOS ONE 13.11(2018):12.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Song, Guibo]'s Articles
[Han, Mingjun]'s Articles
[Li, Zuhua]'s Articles
Baidu academic
Similar articles in Baidu academic
[Song, Guibo]'s Articles
[Han, Mingjun]'s Articles
[Li, Zuhua]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Song, Guibo]'s Articles
[Han, Mingjun]'s Articles
[Li, Zuhua]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.