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题名: Tris (1,3-dichloro-2-propyl) phosphate -induced apoptotic signaling pathways in SH-SY5Y neuroblastoma cells
作者: Li, Ruiwen1, 2; Zhou, Peijiang1; Guo, Yongyong2; Lee, Jae-Seong3; Zhou, Bingsheng2
关键词: SH-SY5Y cells ; Reactive oxygen species ; TDCIPP ; Endoplasmic- reticulum stress ; Apoptosis signaling
刊名: NEUROTOXICOLOGY
发表日期: 2017
DOI: 10.1016/j.neuro.2016.10.018
卷: 58, 页:1-10
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Neurosciences ; Pharmacology & Pharmacy ; Toxicology
研究领域[WOS]: Neurosciences & Neurology ; Pharmacology & Pharmacy ; Toxicology
英文摘要: Tris (1, 3-dichloro-2-propyl) phosphate (TDCIPP, also known as TDCPP), an extensively used flame retardant, is frequently detected in the environment and biota. Recent studies have shown that TDCIPP has neurotoxic effects. In this study, we determined the mechanisms of TDCIPP-induced neurotoxicity in human neuroblastoma (SH-SY5Y) cells. By using morphological examination, flow cytometry, and mitochondrial membrane potential (Delta Ym) measurement, we confirmed that exposure to TDCIPP caused apoptosis accompanied by the activation of apoptosis-related genes (e.g. Bax and Bc1-2) and caspase 3 protein in SH-SY5Y cells. Increased reactive oxygen species (ROS) formation and intracellular calcium ions ([Ca2+](i)) were also observed in TDCIPP-treated SH-SY5Y cells. Exposure to TDCIPP led to the activation of protein markers of endoplasmic reticulum (ER) stress, including eukaryotic translation initiation factor 2a subunit (p-EIF2a), activation transcription factor (ATF4), glucose-regulated protein (GRP78), and the proapoptotic factor C/EBP homologous protein (CHOP). To determine the role of the ER in apoptosis, phenyl butyric acid (PBA), an ER stress inhibitor, was applied. Treatment with PBA effectively attenuated TDCIPP-induced ER stress and protected against apoptotic death in SH-SY5Y cells by inhibition of Bax expression and promotion of Bcl-2 expression. Furthermore, we found that pretreatment of the cells with the ROS scavenger N-acetyl cysteine (NAC) inhibited the ER stress response and prevented apoptosis. The combination of PBA and NAC pretreatment could further prevent TDCIPP induced ER-stress and apoptotic death compared with PBA or NAC pretreatment alone. Thus, in the present study, we demonstrated that TDCIPP induces cytotoxicity through a ROS-dependent mechanism involving ER stress and activation of mitochondrial apoptotic pathways in SH-SY5Y cells. (C) 2016 Elsevier B.V. All rights reserved.
关键词[WOS]: ENDOPLASMIC-RETICULUM STRESS ; ORGANOPHOSPHORUS FLAME RETARDANTS ; HEPATOCELLULAR-CARCINOMA CELLS ; UNFOLDED PROTEIN RESPONSE ; OXIDATIVE STRESS ; ER STRESS ; SAURURUS-CHINENSIS ; PC12 CELLS ; DEATH ; MITOCHONDRIA
语种: 英语
项目资助者: Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14040103) ; National Natural Science Foundation of China(21237005 ; 21577168)
WOS记录号: WOS:000394406400001
ISSN号: 0161-813X
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/28962
Appears in Collections:水环境工程研究中心_期刊论文

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作者单位: 1.Wuhan Univ, Sch Resource & Environm Sci, Hubei Biomass Resource Chem & Environm Biotechnol, Wuhan 430079, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
3.Sungkyunkwan Univ, Dept Biol Sci, Coll Sci, Suwon 16419, South Korea
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