Rapid Functional and Sequence Differentiation of a Tandemly Repeated Species-Specific Multigene Family in Drosophila
Clifton, Bryan D.1; Librado, Pablo2; Yeh, Shu-Dan3; Solares, Edwin S.1; Real, Daphne A.1; Jayasekera, Suvini U.1; Zhang, Wanting4; Shi, Mijuan4; Park, Ronni V.1; Magie, Robert D.1; Ma, Hsiu-Ching1; Xia, Xiao-Qin4; Marco, Antonio5; Rozas, Julio6,7; Ranz, Jose M.1
2017
Source PublicationMOLECULAR BIOLOGY AND EVOLUTION
ISSN0737-4038
Volume34Issue:1Pages:51-65
AbstractGene clusters of recently duplicated genes are hotbeds for evolutionary change. However, our understanding of how mutational mechanisms and evolutionary forces shape the structural and functional evolution of these clusters is hindered by the high sequence identity among the copies, which typically results in their inaccurate representation in genome assemblies. The presumed testis-specific, chimeric gene Sdic originated, and tandemly expanded in Drosophila melanogaster, contributing to increased male-male competition. Using various types of massively parallel sequencing data, we studied the organization, sequence evolution, and functional attributes of the different Sdic copies. By leveraging long-read sequencing data, we uncovered both copy number and order differences from the currently accepted annotation for the Sdic region. Despite evidence for pervasive gene conversion affecting the Sdic copies, we also detected signatures of two episodes of diversifying selection, which have contributed to the evolution of a variety of C-termini and miRNA binding site compositions. Expression analyses involving RNA-seq datasets from 59 different biological conditions revealed distinctive expression breadths among the copies, with three copies being transcribed in females, opening the possibility to a sexually antagonistic effect. Phenotypic assays using Sdic knock-out strains indicated that should this antagonistic effect exist, it does not compromise female fertility. Our results strongly suggest that the genome consolidation of the Sdic gene cluster is more the result of a quick exploration of different paths of molecular tinkering by different copies than a mere dosage increase, which could be a recurrent evolutionary outcome in the presence of persistent sexual selection.
SubtypeArticle
KeywordGene Amplification Functional Diversification Newly Evolved Gene Sdic Drosophila
DOI10.1093/molbev/msw212
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Funding OrganizationNational Science Foundation(MCB-1157876) ; National Science Foundation(MCB-1157876) ; Ministerio de Economia y Competitividad of Spain(CGL2013-45211) ; Ministerio de Economia y Competitividad of Spain(CGL2013-45211) ; National Institutes of Health(R25-GM056647) ; National Institutes of Health(R25-GM056647) ; National Science Foundation(MCB-1157876) ; National Science Foundation(MCB-1157876) ; Ministerio de Economia y Competitividad of Spain(CGL2013-45211) ; Ministerio de Economia y Competitividad of Spain(CGL2013-45211) ; National Institutes of Health(R25-GM056647) ; National Institutes of Health(R25-GM056647)
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Evolutionary Biology ; Genetics & Heredity
WOS SubjectBiochemistry & Molecular Biology ; Evolutionary Biology ; Genetics & Heredity
WOS KeywordDYNEIN INTERMEDIATE-CHAIN ; BASIC LOCAL ALIGNMENT ; SPERM-SPECIFIC GENE ; INSECTICIDE RESISTANCE ; NUCLEOTIDE-SEQUENCES ; DUPLICATED GENES ; MOLECULAR-BASIS ; X-CHROMOSOME ; EVOLUTION ; MELANOGASTER
WOS IDWOS:000396772000004
Funding OrganizationNational Science Foundation(MCB-1157876) ; National Science Foundation(MCB-1157876) ; Ministerio de Economia y Competitividad of Spain(CGL2013-45211) ; Ministerio de Economia y Competitividad of Spain(CGL2013-45211) ; National Institutes of Health(R25-GM056647) ; National Institutes of Health(R25-GM056647) ; National Science Foundation(MCB-1157876) ; National Science Foundation(MCB-1157876) ; Ministerio de Economia y Competitividad of Spain(CGL2013-45211) ; Ministerio de Economia y Competitividad of Spain(CGL2013-45211) ; National Institutes of Health(R25-GM056647) ; National Institutes of Health(R25-GM056647)
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/28955
Collection藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心
Affiliation1.Univ Calif Irvine, Dept Ecol & Evolutionary Biol, Irvine, CA 92717 USA
2.Univ Copenhagen, Nat Hist Museum Denmark, Ctr GeoGenet, Copenhagen, Denmark
3.Natl Cent Univ, Dept Life Sci, Taoyuan, Zhongli Distric, Taiwan
4.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Hubei Province, Peoples R China
5.Univ Essex, Sch Biol Sci, Colchester, Essex, England
6.Univ Barcelona, Dept Genet Microbiol & Estadist, Barcelona, Spain
7.Univ Barcelona, Inst Recerca Biodiversitat, Barcelona, Spain
Recommended Citation
GB/T 7714
Clifton, Bryan D.,Librado, Pablo,Yeh, Shu-Dan,et al. Rapid Functional and Sequence Differentiation of a Tandemly Repeated Species-Specific Multigene Family in Drosophila[J]. MOLECULAR BIOLOGY AND EVOLUTION,2017,34(1):51-65.
APA Clifton, Bryan D..,Librado, Pablo.,Yeh, Shu-Dan.,Solares, Edwin S..,Real, Daphne A..,...&Ranz, Jose M..(2017).Rapid Functional and Sequence Differentiation of a Tandemly Repeated Species-Specific Multigene Family in Drosophila.MOLECULAR BIOLOGY AND EVOLUTION,34(1),51-65.
MLA Clifton, Bryan D.,et al."Rapid Functional and Sequence Differentiation of a Tandemly Repeated Species-Specific Multigene Family in Drosophila".MOLECULAR BIOLOGY AND EVOLUTION 34.1(2017):51-65.
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