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题名: Expression pattern analysis of IRF4 and its related genes revealed the functional differentiation of IRF4 paralogues in teleost
作者: Ai, Kete1, 2; Luo, Kai1; Li, Youshen1; Hu, Wei1; Gao, Weihua1; Fang, Liu1; Tian, Guangming1; Ruan, Guoliang1; Xu, Qiaoqing1, 2
关键词: Interferon regulatory factor 4 (IRF4) ; Paralogues ; Expression ; Modulation
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2017
DOI: 10.1016/j.fsi.2016.11.038
卷: 60, 页:59-64
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
英文摘要: In mammals, interferon regulatory factor 4 (IRF4) plays an important role in the process of development and differentiation of B cells, T cells and dendritic cells. It can regulate immune pathway through IRF5, MyD88, 121, PGC1 alpha, and NOD2. In the present study, we investigated the expression pattern of IRF4 paralogues and these related genes for the first time in teleosts. The results showed that these genes were all expressed predominantly in known immune tissues while IRF5 was also relatively highly expressed in muscle. IRF4b, IL21, MyD88, IRF5 and NOD2 showed maternal expression in the oocyte and the higher expression of IRF4a, Mx and PGC1 alpha before hatching might be involved in the embryonic innate defense system. Zebrafish embryonic fibroblast (ZF4) cells were infected with GCRV and SVCV. During GCRV infection, the expression of Mx was significantly up-regulated from 3 h to 24 h, reaching the highest level at 12 h (101.5-fold over the controls, P < 0.001). And the expression of IRF4a was significantly up-regulated from 3 h to 48 h, reaching the highest level at 12 h (13.75-fold over the controls, P < 0.001). While the expression of IRF4b was only slightly up-regulated at 12 h and 24 h (339 fold, 1.93-fold) above control levels, respectively. Whereas the expression of Mx was significantly up regulated during SVCV infection from 1 h to 48 h, reaching the highest level at 24 h (11.49-fold over the controls, P < 0.001). IRF4a transcripts were significantly up-regulated from 6 h to 24 h, reaching the highest level at 24 h (41 -fold over the controls, P < 0.01). IRF4b only showed a slightly up-regulation by SVCV at 24 h (3.2-fold over the controls, P < 0.01). IRF4a and IRF4b displayed a distinct tissue expression pattern, embryonic stages expression and inducible expression in vivo and in vitro, suggesting that IRF4 paralogues might play different roles in immune system. (C) 2016 Elsevier Ltd. All rights reserved.
关键词[WOS]: INTERFERON REGULATORY FACTORS ; MOLECULAR CHARACTERIZATION ; RAINBOW-TROUT ; IMMUNE ; VIRUS ; CELLS ; RECEPTORS ; EVOLUTION ; SEQUENCE ; CLONING
语种: 英语
项目资助者: National Natural Science Foundation of China(31101928)
WOS记录号: WOS:000392770000009
ISSN号: 1050-4648
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/28938
Appears in Collections:其他_期刊论文

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作者单位: 1.Yangtze Univ, Sch Anim Sci, Jingzhou 434020, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
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