| Morphological Redescription of Opalina undulata Nie 1932 from Fejervarya limnocharis with Molecular Phylogenetic Study of Opalinids (Heterokonta, Opalinea) | |
| Li, Ming1,2; Ponce-Gordo, Francisco3; Grim, J. Norman4; Li, Can1,2; Zou, Hong1,2; Li, Wenxiang1,2; Wu, Shangong1,2; Wang, Guitang1,2 | |
| Corresponding Author | Wang, Guitang(gtwang@ihb.ac.cn) |
| 2018-11-01 | |
| Source Publication | JOURNAL OF EUKARYOTIC MICROBIOLOGY
 |
| ISSN | 1066-5234 |
| Volume | 65Issue:6Pages:783-791 |
| Abstract | The redescription of Opalina undulata Nie 1932, collected from the rectum of the frog Fejervarya limnocharis, is presented in this paper based on detailed morphological information and molecular data. Our results revealed that specimens collected from Diaocha Lake in late August were larger and had more nuclei than those collected from the same site in early May. We sequenced their SSU rDNA-ITS1-5.8S rDNA-ITS2-LSU rDNA (5 ' end) and found that they were completely identical, which means that the two populations belonged to the same species. These facts gave us a hint that body dimension and number of nuclei are not reliable taxonomic parameters for opalinids during their life cycle. Therefore, we recommended that the specific identification of opalinids based on morphological features should be carried out during seasons except spring. Meanwhile, our molecular phylogenetic analysis confirmed the monophyly of Opalinata. Within Opalinata, Opalinea were monophyletic with all opalinid species grouping together. Karotomorpha and Proteromonas did not group together confirming the paraphyly of Proteromonadea. |
| Keyword | ITS region LSU rDNA morphology phylogenetic analysis SSU rDNA |
| DOI | 10.1111/jeu.12520 |
| Funding Organization | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System |
| Indexed By | SCI ; SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[31772429] ; National Natural Science Foundation of China[31471978] ; Youth Innovation Promotion Association CAS[Y82Z01] ; earmarked fund for China Agriculture Research System[CARS-45-15] |
| WOS Research Area | Microbiology |
| WOS Subject | Microbiology |
| WOS ID | WOS:000451783600003 |
| WOS Keyword | REVISED CLASSIFICATION ; GC CONTENT ; CLUSTAL-W ; POSITION ; MODEL ; ITS2 |
| Publisher | WILEY |
| Funding Organization | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; Youth Innovation Promotion Association CAS ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System ; earmarked fund for China Agriculture Research System |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/28917 |
| Collection | 鱼类生物学及渔业生物技术研究中心_期刊论文 |
| Corresponding Author | Wang, Guitang |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquaculture Dis Control, Minist Agr, Wuhan 430072, Hubei, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 3.Univ Complutense Madrid, Fac Farm, Dept Microbiol & Parasitol, Plaza Ramony Cajal S-N, E-28040 Madrid, Spain 4.No Arizona Univ, Dept Biol Sci, Box 5640, Flagstaff, AZ 86011 USA |
| Recommended Citation GB/T 7714 | Li, Ming,Ponce-Gordo, Francisco,Grim, J. Norman,et al. Morphological Redescription of Opalina undulata Nie 1932 from Fejervarya limnocharis with Molecular Phylogenetic Study of Opalinids (Heterokonta, Opalinea)[J]. JOURNAL OF EUKARYOTIC MICROBIOLOGY,2018,65(6):783-791. |
| APA | Li, Ming.,Ponce-Gordo, Francisco.,Grim, J. Norman.,Li, Can.,Zou, Hong.,...&Wang, Guitang.(2018).Morphological Redescription of Opalina undulata Nie 1932 from Fejervarya limnocharis with Molecular Phylogenetic Study of Opalinids (Heterokonta, Opalinea).JOURNAL OF EUKARYOTIC MICROBIOLOGY,65(6),783-791. |
| MLA | Li, Ming,et al."Morphological Redescription of Opalina undulata Nie 1932 from Fejervarya limnocharis with Molecular Phylogenetic Study of Opalinids (Heterokonta, Opalinea)".JOURNAL OF EUKARYOTIC MICROBIOLOGY 65.6(2018):783-791. |
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