| Transcriptome from Pacific cod liver reveals types of apolipoproteins and expression analysis of AFP-IV, structural analogue with mammalian ApoA-I | |
| Mao, Ming-Guang1,2; Chen, Yu1; Liu, Rui-Ting1; Lu, Hui-Qian1; Gu, Jie1; Jiang, Zhi-Qiang1; Jiang, Jie-Lan1 | |
| Corresponding Author | Jiang, Jie-Lan(jielanjiang@dlou.edu.cn) |
| 2018-12-01 | |
| Source Publication | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
 |
| ISSN | 1744-117X |
| Volume | 28Pages:204-212 |
| Abstract | Apolipoproteins (Apos), transporting the lipids through the lymphatic and circulatory systems, are associated with kinds of diseases. Additionally, type IV antifreeze protein (AFP-IV) was related evolutionarily with apolipoproteins. However, the information of Apos in fish was limited. In this study, ApoA-I, ApoA-I-2, ApoA-IV, Apo E, ApoB-100-like and AFP-IV were sequenced from Pacific cod (Gadus macrocephalus) liver transcriptome using Illumina HiSeq 2000, and their 3-D models were constructed based on the most confidence templates ever reported in mammals. Interestingly, the model of G. macrocephalus AFP-IV, named GmAFPIV, is quite similar to the structure of ApoA-I. GrnAFPIV includes 689 bases with a complete open reading frame encoding 125 amino acids. Sequence alignment of GmAFPIV showed 30% to 50% similarity with that of other species except Gadus sp. Expression levels of GmAFPIV were found in a decreasing manner in liver, intestine, gill, brain and gonad. Heterologously expression of the GmAFPIV protein was expressed in Escherichia coli and purified to immunize New Zealand rabbits. The survivors of E. coli in 60 mu g/mL of GmAFPIV are more than that in the 30 mu g/mL group after stored in -20 degrees C and -80 degrees C, indicating high concentration of GmAFPIV could protect E. coli avoiding the damage from ice crystal. The subcellular localization of GmAFPIV showed that the green fluorescence was mainly observed in the cytoplasm, indicating GmAFPIV play roles in the cytoplasm. It was concluded that GrnAFPIV may function not only as an antifreeze protein but also as an apolipoprotein transporting lipids in fish. |
| Keyword | Apolipoprotein Gadus macrocephalus Type-IV antifreeze protein ApoA-I homolog Transcriptome |
| DOI | 10.1016/j.cbd.2018.10.001 |
| Funding Organization | Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China |
| Indexed By | SCI ; SCI |
| Language | 英语 |
| Funding Project | Foundation of the Education Department of Liaoning Province[LR2016009] ; Foundation of the Education Department of Liaoning Province[QL201702] ; Foundation of the Education Department of Liaoning Province[L2015076] ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS[2017HY-XKQ01-08] ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China[2018-KF-09] |
| WOS Research Area | Biochemistry & Molecular Biology ; Genetics & Heredity |
| WOS Subject | Biochemistry & Molecular Biology ; Genetics & Heredity |
| WOS ID | WOS:000452567900021 |
| WOS Keyword | ANTIFREEZE PROTEINS ; A-I ; LONGHORN SCULPIN ; PRIMARY DEFENSE ; GENE ; ZEBRAFISH ; HDL ; SEQUENCE ; DOMAIN ; MODEL |
| Publisher | ELSEVIER SCIENCE INC |
| Funding Organization | Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/28895 |
| Collection | 其他_期刊论文 |
| Corresponding Author | Jiang, Jie-Lan |
| Affiliation | 1.Dalian Ocean Univ, Key Lab Mariculture & Stock Enhancement North Chi, Minist Agr & Rural Affairs,Coll Fisheries & Life, Key Lab Fish Appl Biol & Aquaculture North China, Dalian 116023, Liaoning, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China |
| Recommended Citation GB/T 7714 | Mao, Ming-Guang,Chen, Yu,Liu, Rui-Ting,et al. Transcriptome from Pacific cod liver reveals types of apolipoproteins and expression analysis of AFP-IV, structural analogue with mammalian ApoA-I[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,2018,28:204-212. |
| APA | Mao, Ming-Guang.,Chen, Yu.,Liu, Rui-Ting.,Lu, Hui-Qian.,Gu, Jie.,...&Jiang, Jie-Lan.(2018).Transcriptome from Pacific cod liver reveals types of apolipoproteins and expression analysis of AFP-IV, structural analogue with mammalian ApoA-I.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,28,204-212. |
| MLA | Mao, Ming-Guang,et al."Transcriptome from Pacific cod liver reveals types of apolipoproteins and expression analysis of AFP-IV, structural analogue with mammalian ApoA-I".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS 28(2018):204-212. |
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