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Transcriptome from Pacific cod liver reveals types of apolipoproteins and expression analysis of AFP-IV, structural analogue with mammalian ApoA-I
Mao, Ming-Guang1,2; Chen, Yu1; Liu, Rui-Ting1; Lu, Hui-Qian1; Gu, Jie1; Jiang, Zhi-Qiang1; Jiang, Jie-Lan1
Corresponding AuthorJiang, Jie-Lan(jielanjiang@dlou.edu.cn)
2018-12-01
Source PublicationCOMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
ISSN1744-117X
Volume28Pages:204-212
AbstractApolipoproteins (Apos), transporting the lipids through the lymphatic and circulatory systems, are associated with kinds of diseases. Additionally, type IV antifreeze protein (AFP-IV) was related evolutionarily with apolipoproteins. However, the information of Apos in fish was limited. In this study, ApoA-I, ApoA-I-2, ApoA-IV, Apo E, ApoB-100-like and AFP-IV were sequenced from Pacific cod (Gadus macrocephalus) liver transcriptome using Illumina HiSeq 2000, and their 3-D models were constructed based on the most confidence templates ever reported in mammals. Interestingly, the model of G. macrocephalus AFP-IV, named GmAFPIV, is quite similar to the structure of ApoA-I. GrnAFPIV includes 689 bases with a complete open reading frame encoding 125 amino acids. Sequence alignment of GmAFPIV showed 30% to 50% similarity with that of other species except Gadus sp. Expression levels of GmAFPIV were found in a decreasing manner in liver, intestine, gill, brain and gonad. Heterologously expression of the GmAFPIV protein was expressed in Escherichia coli and purified to immunize New Zealand rabbits. The survivors of E. coli in 60 mu g/mL of GmAFPIV are more than that in the 30 mu g/mL group after stored in -20 degrees C and -80 degrees C, indicating high concentration of GmAFPIV could protect E. coli avoiding the damage from ice crystal. The subcellular localization of GmAFPIV showed that the green fluorescence was mainly observed in the cytoplasm, indicating GmAFPIV play roles in the cytoplasm. It was concluded that GrnAFPIV may function not only as an antifreeze protein but also as an apolipoprotein transporting lipids in fish.
KeywordApolipoprotein Gadus macrocephalus Type-IV antifreeze protein ApoA-I homolog Transcriptome
DOI10.1016/j.cbd.2018.10.001
Funding OrganizationFoundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China
Indexed BySCI ; SCI
Language英语
Funding ProjectFoundation of the Education Department of Liaoning Province[LR2016009] ; Foundation of the Education Department of Liaoning Province[QL201702] ; Foundation of the Education Department of Liaoning Province[L2015076] ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS[2017HY-XKQ01-08] ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China[2018-KF-09]
WOS Research AreaBiochemistry & Molecular Biology ; Genetics & Heredity
WOS SubjectBiochemistry & Molecular Biology ; Genetics & Heredity
WOS IDWOS:000452567900021
WOS KeywordANTIFREEZE PROTEINS ; A-I ; LONGHORN SCULPIN ; PRIMARY DEFENSE ; GENE ; ZEBRAFISH ; HDL ; SEQUENCE ; DOMAIN ; MODEL
PublisherELSEVIER SCIENCE INC
Funding OrganizationFoundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Foundation of the Education Department of Liaoning Province ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; Central Public-interest Scientific Institution Basal Research & Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs of the People's Republic of China, CAFS ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China ; open project of Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs of the People's Republic of China
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/28895
Collection其他_期刊论文
Corresponding AuthorJiang, Jie-Lan
Affiliation1.Dalian Ocean Univ, Key Lab Mariculture & Stock Enhancement North Chi, Minist Agr & Rural Affairs,Coll Fisheries & Life, Key Lab Fish Appl Biol & Aquaculture North China, Dalian 116023, Liaoning, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
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Mao, Ming-Guang,Chen, Yu,Liu, Rui-Ting,et al. Transcriptome from Pacific cod liver reveals types of apolipoproteins and expression analysis of AFP-IV, structural analogue with mammalian ApoA-I[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,2018,28:204-212.
APA Mao, Ming-Guang.,Chen, Yu.,Liu, Rui-Ting.,Lu, Hui-Qian.,Gu, Jie.,...&Jiang, Jie-Lan.(2018).Transcriptome from Pacific cod liver reveals types of apolipoproteins and expression analysis of AFP-IV, structural analogue with mammalian ApoA-I.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,28,204-212.
MLA Mao, Ming-Guang,et al."Transcriptome from Pacific cod liver reveals types of apolipoproteins and expression analysis of AFP-IV, structural analogue with mammalian ApoA-I".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS 28(2018):204-212.
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