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题名: Role of zebrafish NLRC5 in antiviral response and transcriptional regulation of MHC related genes
作者: Wu, Xiao Man1, 2; Hu, Yi Wei1, 2; Xue, Na Na1, 2; Ren, Shi Si1, 2; Chen, Shan Nan1; Nie, Pin1; Chang, Ming Xian1
关键词: Zebrafish ; NLRC5 ; SVCV infection ; Antiviral activity ; MHC class II transactivator
刊名: DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
发表日期: 2017-03-01
DOI: 10.1016/j.dci.2016.11.018
卷: 68, 页:58-68
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Immunology ; Zoology
研究领域[WOS]: Immunology ; Zoology
英文摘要: Intracellular NOD-like receptors (NLRs) are emerging as critical regulators of innate and adaptive immune responses. Although the NLR family member NLRC5 is functionally defined, the role of NLRC5 in regulating innate immune signaling has been controversial in mammals, and is poorly understood in teleost fish. In the present study, we report the functional characterization of zebrafish NLRC5. The cloned NLRC5 consists of 6435 bp which encodes 1746 amino acids. The N-terminal effector-binding domain of zebrafish NLRC5 is absent which is different from all other human NLRs. Fluorescence microscopy showed that zebrafish NLRC5 is located throughout the entire cell. The higher expression of zebrafish NLRC5 in embryo than in larvae was observed, suggesting the action phase of NLRC5 in zebrafish ontogehetic stages. When the modulation of NLRC5 in pathogen infection was analyzed, it was found that zebrafish NLRC5 was upregulated by both bacterial and viral infection. Overexpression of zebrafish NLRC5 resulted in significant inhibition of SVCV replication in vivo and in vitro, but failed to activate interferon (IFN) promoters and type I IFN signaling pathway. Interestingly, NLRC5 overexpression could activate mhc2dab promoter, and induce the expression of MHC class II genes. All together, these results demonstrate that zebrafish NLRC5 is involved in IFN-independent antiviral response, and also functions as a transcriptional regulator of MHC class II genes. (C) 2016 Elsevier Ltd. All rights reserved.
关键词[WOS]: CD44 DEFICIENCY LEADS ; FAMILY-MEMBER NLRC5 ; NOD-LIKE RECEPTORS ; RIG-I ; CONTAINING PROTEINS ; VIRUS-INFECTION ; HOST-DEFENSE ; EXPRESSION ; INFLAMMATION ; TRANSACTIVATOR
语种: 英语
项目资助者: National Natural Science Foundation of China(31372531 ; 30830083)
WOS记录号: WOS:000392683700007
ISSN号: 0145-305X
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/28881
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
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